Ultrastructural changes in the mycelia of Actinomyces hygroscopicus var. var. enhygrus--a proteolytic enzyme producer--during submerged fermentation

Ultrastructural changes were found to occur in the mycelium of Actinomyces hygroscopicus, strain 33x, which produced an exocellular proteolytic enzyme during submerged fermentation, in both laboratory and semiindustrial conditions (in 100-1 fermenters). In the course of the enzyme accumulation, numerous vacuoles appeared in the hyphae, some hyphae became wider, and the cell walls were more loose. The greatest structural changes were found in the intracytoplasmic membrane systems. The ultrastructural changes of the mycelium are not presumably connected with the accumulation of the enzyme, but are the result of the differentiation of cellular structures during aging of the cells and their transition to the stage of autolysis.     

A restatement and test of Schlosberg's theory of emotion with two kinds of subjects from Greece.

Schlosberg's dimensional theory of emotion, including the dimension of tension-sleep, is tested to compare results obtained previously with American college students with those obtained in this study using Greek Ss. 15 Greek villagers and 15 college students in Athens comprised the 2 experimental groups which differed from each other in educational level and in lifetime movie attendance. The data indicate that Schlosberg's conclusions are now valid for non-Western groups as well as Western. Furthermore an emotion solid can be plotted from data collected by making use of Schlosberg's procedure, but the shape of the solid is not the same as that previously presented by Schlosberg. 18 references. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Application of Response Surface Methodology to the Selection of Machining Variables

This paper presents the use of Response Surface Methodology for analysis of material removal processes. Mathematical models of tool life, surface finish and cutting force are developed in terms of cutting speed, feed, and depth of cut. The models were then used to select the levels of the machining variables such that certain criteria could be achieved. Specific examples include increasing the rate of metal removal without sacrificing surface finish and with a minimum loss in tool life.     

Differences in tissue fatty acids and cholesterol of swine from different genetic backgrounds

A review of 1,000 consecutive coronary angiograms, most of them performed for evaluation of angina pectoris, yielded 9 examples of congenital anomalies of the coronary arteries. In 2 cases the angina may have been due to malposition of the left coronary artery or one of its branches. There were 2 cases of aberrant origin of the circumflex artery from the right coronary artery, 2 cases of aberrant left anterior descending artery, 3 cases in which all three major coronary branches arose from the right aortic sinus, and 2 cases of coronary artery fistulas. Malposition of the coronary artery should be considered as a possible cause of angina.     

Decision aid for allocation of transportation funds to guardrails

We address the need for allocation of resources to run-off-road and fixed-object hazards on immense secondary road systems. In Virginia, there are 95,000km of roadway with uncharacterized hazards in need of guardrail upgrade, installation, or related warning signs or other protection. A decision aid is developed to assist the planner in guardrail resource allocation by accounting for the potential crash severities, traffic exposures, costs of treatment, and other factors. A premise is that no single benefit-cost ratio or selection criterion applies across all localities. The decision aid enables the planner to interpret the variety of benefits and costs in their own units, emphasizing the needs and preferences of individual localities. The paper describes: (1) archiving and comparison of protected and unprotected hazards; (2) regional screening of hazardous corridors and (3) multicriteria benefit-cost analyses of guardrail sites. A case study of guardrail selection is presented.     

A literature review of portable fluorescence-based oil-in-water monitors

The results of a literature search on fluorescence-based portable detectors to measure the real-time concentration of oil are reported. For more than two decades, fluorometers have been commonly employed to monitor dispersed oil levels at oil spills on water. The focus of this paper has been to extract specific information from references about how the instruments were used, including set up and calibration procedures, the oil and dispersant measured, the approximate concentration range of the oil in the water column, and how the real-time data compared to traditional laboratory techniques.     

Solvent vapour monitoring in work space by solid phase micro extraction

Solid phase micro extraction (SPME) is a fast, solvent-less alternative to conventional charcoal tube sampling/carbon disulfide extraction for volatile organic compounds (VOC). In this work, SPME was compared to the active sampling technique in a typical lab atmosphere. Two different types of fibre coatings were evaluated for solvent vapour at ambient concentration. A general purpose 100 microm film polydimethylsiloxane (PDMS) fibre was found to be unsuitable for VOC work, despite the thick coating. The mixed-phase carboxen/PDMS fibre was found to be suitable. Sensitivity of the SPME was far greater than charcoal sorbent tube method. Calibration studies using typical solvent such as dichloromethane (DCM), benzene (B) and toluene (T) showed an optimal exposure time of 5 min, with a repeatability of less than 20% for a broad spectrum of organic vapour. Minimum detectable amount for DCM is in the range of 0.01 microg/l (0.003 ppmv). Variation among different fibres was generally within 30% at a vapour concentration of 1 microg DCM/l, which was more than adequate for field monitoring purpose. Adsorption characteristics and calibration procedures were studied. An actual application of SPME was carried out to measure background level of solvent vapour at a bench where DCM was used extensively. Agreement between the SPME and the charcoal sampling method was generally within a factor of two. No DCM concentration was found to be above the regulatory limit of 50 ppmv.