Selective deposits of versican in the extracellular matrix of restenotic lesions from human peripheral arteries

Although a large percentage of the volume of human restenotic arterial lesions is occupied by extracellular matrix (ECM), the composition and organization of this ECM are not well characterized. In this study, restenotic segments taken from 30 human peripheral arteries by directional atherectomy at times ranging from 13 days to 36 months after angioplasty were analyzed for specific patterns of ECM composition and organization by light and electron microscopic histochemistry and immunohistochemistry. Histochemical analysis revealed the presence of distinct zones, enriched either in proteoglycans or fibrillar collagen. Most sections contained these regions juxtaposed to each other. The frequency of these two distinct ECMs did not change as a function of time after angioplasty. The collagen-rich zone usually contained elongated smooth muscle cells spaced close together except in regions resembling fibrous plaques. The proteoglycan-rich ECM contained both elongated and stellate-shaped smooth muscle cells randomly arranged and separated by wide distances. This region resembled the loose-connective-tissue-containing myxoid region typical of restenotic lesions. Immunohistochemical analysis of these regions revealed that the proteoglycan-containing zone stained intensely for versican, a large interstitial chondroitin sulfate proteoglycan, whereas the collagen-containing areas were mostly negative for versican but positive for type I collagen. The versican-positive regions also immunostained for biglycan, a small leucine-rich dermatan sulfate proteoglycan, and sparsely for elastin. However, both of these ECM molecules were present in the versican-negative type I collagen-positive regions of the lesions. These results suggest that the development of restenotic lesions involves localized deposits of specific ECM molecules that may play a role in the asymmetric renarrowing of this tissue after angioplasty.     

Experimental investigation of some hydrodynamic factors affecting slurry pipeline wall erosion

Using a non-intrusive technique, particle velocities and concentrations have been measured as functions of position on the pipe wall for water slurries of two different sands. The experimental conditions correspond to both low (fine particles) and high contact load flows. Although the velocity and concentration distributions for the two sands were quite different, flux distributions computed from the product of velocity and concentration were similar. The flux distributions are used to interpret wall erosion distributions for sands of these sizes. In the case of the coarser sand, there is evidence that the contact load mechanism contributes significantly to abrasive wear. In this case, scaleup of erosion test results may be possible, using concepts provided by mechanistic flow models. For the finer sand, wall erosion rates appear to be reduced by a lubrication force.     

Bayesian model for flow-class dependent distributions of fecal-indicator bacterial concentration in surface waters

A Bayesian statistical water quality model is demonstrated to predict fecal-indicator bacterial concentrations for waterbodies without sufficient monitoring data for data-intensive modeling techniques. Using a truncated bivariate normal likelihood function and the readily available observations of flow and bacterial concentration, the Bayesian approach propagates the uncertainty in the model parameterization to the final predictions of in-stream bacterial concentration. The proposed model captures the variation in the magnitude of bacterial loading between dry and wet conditions by estimating separate sets of model parameters for different flow conditions, but also has the capability to pool the data among flow conditions. The model can be used in two ways: first, the model specifies the percent of time that the recreational season in-stream concentration is not in compliance with the relevant water quality standard, and second, the model estimates the necessary bacterial load reduction for multiple flow conditions to meet the relevant water quality standard. Using an eleven year monitoring record for a site sampled at a monthly frequency on the Youghiogheny River in southwestern Pennsylvania, USA, the model parameters are updated and posterior predictions are generated for each 2-year increment. After six years of sampling, the predicted percent of time that the recreational season in-stream bacterial concentration is not in compliance with the relevant water quality is 82% with 95% CI(80,85), and the bacterial load reductions required to meet the standard are 14.7 CI(14.6,14.8), 14.5 CI(14.3, 14.6), and 14.0 CI(13.9, 14.2) log₁₀(cfu/day) for the high, normal, and dry flow conditions, respectively. The change in estimated load reduction and percent exceedance resulting from additional monitoring for this site becomes small after six years of sampling, indicating that a decision does not need to be postponed for additional monitoring.     

Microbially mediated mineral carbonation: roles of phototrophy and heterotrophy

Ultramafic mine tailings from the Diavik Diamond Mine, Canada and the Mount Keith Nickel Mine, Western Australia are valuable feedstocks for sequestering CO? via mineral carbonation. In microcosm experiments, tailings were leached using various dilute acids to produce subsaline solutions at circumneutral pH that were inoculated with a phototrophic consortium that is able to induce carbonate precipitation. Geochemical modeling of the experimental solutions indicates that up to 2.5% and 16.7% of the annual emissions for Diavik and Mount Keith mines, respectively, could be sequestered as carbonate minerals and phototrophic biomass. CO? sequestration rates are mainly limited by cation availability and the uptake of CO?. Abundant carbonate mineral precipitation occurred when heterotrophic oxidation of acetate acted as an alternative pathway for CO? delivery. These experiments highlight the importance of heterotrophy in producing sufficient DIC concentrations while phototrophy causes alkalinization of waters and produces biomass (fatty acids = 7.6 wt.%), a potential feedstock for biofuel production. Tailings storage facilities could be redesigned to promote CO? sequestration by directing leachate waters from tailings piles into specially designed ponds where carbonate precipitation would be mediated by both chemical and biological processes, thereby storing carbon in stable carbonate minerals and potentially valuable biomass.     

Inorganic Precipitation of Calcite in Mine Tailings Using Trona

Mine Water and the Environment - Mine tailings pose environmental risks such as dust emission and acid mine drainage (AMD) and also present geotechnical risks, i.e. tailings dam failures. In-situ...