Continuous-Time Negotiation Mechanism for Software Agents

While there are several existing mechanisms and systems addressing the crucial and difficult issues of automated one-to-many negotiation, this paper develops a flexible one-to-many negotiation mechanism for software agents. Unlike the existing general one-to-many negotiation mechanism, in which an agent should wait until it has received proposals from all its trading partners before generating counterproposals, in the flexible one-to-many negotiation mechanism, an agent can make a proposal in a flexible way during negotiation, i.e., negotiation is conducted in continuous time. To decide when to make a proposal, two strategies based on fixed waiting time and a fixed waiting ratio is proposed. Results from a series of experiments suggest that, guided by the two strategies for deciding when to make a proposal, the flexible negotiation mechanism achieved more favorable trading outcomes as compared with the general one-to-many negotiation mechanism. To determine the amount of concession, negotiation agents are guided by four mathematical functions based on factors such as time, trading partners' strategies, negotiation situations of other threads, and competition. Experimental results show that agents guided by the four functions react to changing market situations by making prudent and appropriate rates of concession and achieve generally favorable negotiation outcomes     

Integrated position estimation using aerial image sequences

Presents an integrated system for navigation parameter estimation using sequential aerial images, where the navigation parameters represent the positional and velocity information of an aircraft for autonomous navigation. The proposed integrated system is composed of two parts: relative position estimation and absolute position estimation. Relative position estimation recursively computes the current position of an aircraft by accumulating relative displacement estimates extracted from two successive aerial images. Simple accumulation of parameter values reduces the reliability of the extracted parameter estimates as an aircraft goes on navigating, resulting in a large positional error. Therefore, absolute position estimation is required to compensate for the positional error generated by the relative position estimation. Absolute position estimation algorithms using image matching and digital elevation model (DEM) matching are presented. In the image matching, a robust-oriented Hausdorff measure (ROHM) is employed, whereas in the DEM matching, an algorithm using multiple image pairs is used. Experiments with four real aerial image sequences show the effectiveness of the proposed integrated position estimation algorithm     

Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast

Twenty-eight site-directed mutations were introduced into the fission yeast gene (pcn1+) that encodes proliferating cell nuclear antigen (PCNA) and their in vivo effects analyzed in a strain with a null pcn1 background. Mutants defective in enhancing processivity of DNA polymerase delta have previously been identified. In this study, we assessed all of the mutants for their sensitivities to temperature, hydroxyurea, UV irradiation and methyl methanesulfonate (MMS), and specific mutants were also tested for sensitivity to gamma irradiation. One cold-sensitive allele, pcn1-3, was characterized in detail. This mutant had a recessive cold-sensitive cdc phenotype and showed sensitivity to hydroxyurea, UV, and gamma irradiation. At the non-permissive temperature pcn1-3 protein was able to form homotrimers in solution and showed increased stimulation of both synthetic activity and processivity of DNA polymerase delta relative to the wild-type Pcn1+ protein. Epistasis analyses indicated that pcn1-3 is defective in the repair pathway involving rad2+ but not defective in the classical nucleotide excision repair pathway involving rad13+. Furthermore, pcn1-3 is either synthetically or conditionally lethal in null checkpoint rad backgrounds and displays a mitotic catastrophe phenotype in these backgrounds. A model for how pcn1-3 defects may affect DNA repair and replication is presented.     

Variation in phenol content in grapes during ripening: low-molecular-weight phenols

Summary Variations in low-molecular-weight phenolic compounds in different parts (must, skin, and seeds) of berries ofV. vinifera, variety Cencibel during ripening were studied using HPLC andTLC. Twenty-five different compounds were identified. The differences were chiefly quantitative, although some quantitative differences were also recorded.

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Veränderungen des Phenolgehalts während der Reife der Weintrauben: Polyphenole mit niedrigem Molekulargewicht

Zusammenfassung Die Veränderungen der phenolischen Verbindungen mit niedrigem Molekulargewicht während der Reife in verschiedenen Teilen (Saft, Schale und Samen) der Beeren vonV. vinifera, Varietät Cencibel, wurden mit HPLC- und DS-Chromatographie untersucht. Fünfundzwanzig Verbindungen wurden identifiziert. Die Veränderungen waren hauptsächlich quantitativ; einige qualitative Veränderungen wurden ebenfalls beobachtet.

     

Magnetic resonance images of neuronal migration anomalies

Neuronal migration anomalies are a spectrum of brain malformations caused by insults to migrating neuroblasts during the sixth week to fifth month of gestation. To study the characteristics of MRI findings in migration anomalies, MR images of 36 patients (28 children and 8 adults) with migration anomalies were evaluated. Five patients had lissencephaly, eight had pachygyria, twelve had schizencephaly, six had heterotopias of gray matter, three had hemimegalencephaly, and two had polymicrogyria. The frequency of migration anomalies was 0.51% of all cranial MRI studies and 1.21% of pediatric cranial MRI studies at our hospital. The major clinical presentations of these patients were seizure (64%), development delay (42%), motor deficits (42%) and mental retardation (25%). Twenty-five patients (69%) associated with other brain anomalies, including: other migration anomalies in 12 cases (33%), absence of the septum pellucidum in 10 cases (28%), Dandy-Walker malformation/variant in 5 cases, arachnoid cyst in 4 cases, agenesis of the corpus callosum in 3 cases, holoprosencephaly in 2 cases, mega cisterna magna in 1 case and cephalocele in 1 case. Some of them presented with multiple complicated anomalies. As MR imaging provides superb gray-white matter distinction, details of cortical anatomy and multiplanar capability, it can clearly delineate the detail morphologic changes of the brain caused by neuronal migration disorders as well as the associated anomalies.     

Evaluation of atypical cytochrome P450 kinetics with two-substrate models: evidence that multiple substrates can simultaneously bind to cytochrome P450 active sites

Some cytochrome P450 catalyzed reactions show atypical kinetics, and these kinetic processes can be grouped into five categories: activation, autoactivation, partial inhibition, substrate inhibition, and biphasic saturation curves. A two-site model in which the enzyme can bind two substrate molecules simultaneously is presented which can be used to describe all of these observed kinetic properties. Sigmoidal kinetic characteristics were observed for carbamazepine metabolism by CYP3A4 and naphthalene metabolism by CYPs 2B6, 2C8, 2C9, and 3A5 as well as dapsone metabolism by CYP2C9. Naphthalene metabolism by CYP3A4 and naproxen metabolism by CYP2C9 demonstrated nonhyperbolic enzyme kinetics suggestive of a low Km, low Vmax component for the first substrate molecule and a high Km, high Vmax component for the second substrate molecule. 7, 8-Benzoflavone activation of phenanthrene metabolism by CYP3A4 and dapsone activation of flurbiprofen and naproxen metabolism by CYP2C9 were also observed. Furthermore, partial inhibition of 7, 8-benzoflavone metabolism by phenanthrene was observed. These results demonstrate that various P450 isoforms may exhibit atypical enzyme kinetics depending on the substrate(s) employed and that these results may be explained by a model which includes simultaneous binding of two substrate molecules in the active site.