Validation of temperature imaging by H2O absorption spectroscopy using hyperspectral tomography in controlled experiments

This paper describes a preliminary demonstration and validation of temperature imaging using hyperspectral H2O absorption tomography in controlled experiments. Fifteen wavelengths are monitored on each of 30 laser beams to reconstruct the temperature image in a 381 mm × 381 mm square room-temperature plane that contains a 102 mm × 102 mm square zone of lower or higher temperature. The hyperspectral tomography technique attempts to leverage multispectral information to enhance measurement fidelity. The experimental temperature images exhibit average accuracies of 2.3% or better, with pixel-by-pixel standard deviations of less than 1%. In addition, even when the internal zone is only 4 K cooler than the surroundings, its presence is still detectable; statistical analysis of the associated experimental image reveals a 98% confidence that the internal zone is in fact cooler than the surroundings.     

Hydrodynamic interaction between two red blood cells in simple shear flow: its impact on the rheology of a semi-dilute suspension

Blood is a suspension of red blood cells (RBCs) and its rheology is important when discussing the physiology of the cardiovascular system. In this study, we performed a numerical investigation of the rheological properties of an RBC suspension from the dilute to semi-dilute regime. RBCs were modelled as a capsule with a two-dimensional hyperelastic membrane. Large deformation of the thin membrane was calculated by a finite element method. Due to the small size of the RBC, fluid motion around the RBC was assumed to follow Stokes flow and was solved by a boundary element method. In the dilute limit, cell–cell interactions were omitted and the bulk stress of the suspension was calculated by the stresslet generated on a single RBC. Interestingly, the effective shear viscosity of the dilute suspension decreased with increasing viscosity of the internal liquid. In the semi-dilute regime, cells can be considered as showing pairwise interactions. The effective shear viscosity of the semi-dilute suspension shows a quadratic increase with respect to the volume fraction. These findings are important for understanding the complex phenomena of blood rheology.     

Design and operation of an air-conditioning system fueled by wood pellets

A room-cooling system of 2 kW capacity fueled by wood pellets was designed, built and tested. The system was demonstrated during summer at the Yakushima Field Station of Kagoshima University, Japan. It contained a pellet feeder, a pellet burner, a heat exchanger, a lithium bromide–water absorption heat pump and a control unit. The volume of the test room was 36.9 m³ and ambient temperature 30 °C. The airflow temperature from the room unit was decreased to 16 °C by the system, and the room temperature could be successfully controlled to 24 °C steady state. Room heating in winter was also demonstrated. Since the air was heat exchanged, the overall energy efficiency of the cooling system was low at about 19%. However, the calculation based on the heat flow showed that the efficiency could be enhanced to about 75% by direct heating of the regenerator by the flue gas.     

Adenylate Kinase Isozyme 3 Regulates Mitochondrial Energy Metabolism and Knockout Alters HeLa Cell Metabolism

The balance between oxidative phosphorylation and glycolysis is important for cancer cell growth and survival, and changes in energy metabolism are an emerging therapeutic target. Adenylate kinase (AK) regulates adenine nucleotide metabolism, maintaining intracellular nucleotide metabolic homeostasis. In this study, we focused on AK3, the isozyme localized in the mitochondrial matrix that reversibly mediates the following reaction: Mg²⁺ GTP + AMP ⇌ Mg²⁺ GDP + ADP. Additionally, we analyzed AK3-knockout (KO) HeLa cells, which showed reduced proliferation and were detected at an increased number in the G1 phase. A metabolomic analysis showed decreased ATP; increased glycolytic metabolites such as glucose 6 phosphate (G6P), fructose 6 phosphate (F6P), and phosphoenolpyruvate (PEP); and decreased levels of tricarboxylic acid (TCA) cycle metabolites in AK3KO cells. An intracellular ATP evaluation of AK3KO HeLa cells transfected with ATeam plasmid, an ATP sensor, showed decreased whole cell levels. Levels of mitochondrial DNA (mtDNA), a complementary response to mitochondrial failure, were increased in AK3KO HeLa cells. Oxidative stress levels increased with changes in gene expression, evidenced as an increase in related enzymes such as superoxide dismutase 2 (SOD2) and SOD3. Phosphoenolpyruvate carboxykinase 2 (PCK2) expression and PEP levels increased, whereas PCK2 inhibition affected AK3KO HeLa cells more than wild-type (WT) cells. Therefore, we concluded that increased PCK2 expression may be complementary to increased GDP, which was found to be deficient through AK3KO. This study demonstrated the importance of AK3 in mitochondrial matrix energy metabolism.     

Microsatellite instability in B-cell lymphoma originating from Bloom syndrome

We present two cases of osteochondroma after total body irradiation in bone marrow recipients, the first in a 6-year-old boy with juvenile chronic myelogenous leukemia and the second in a 13-year-old boy with acute myelogenous leukemia. The patients developed multiple osteochondromas three years and seven years, respectively, after 12 Gy of total body irradiation. Neither had a family history of hereditary multiple osteochondromatosis. A review of the English literature revealed only one report describing five cases of osteochondroma after 12 Gy of total body irradiation in bone marrow transplant recipients. Osteochondroma should be considered as an additional adverse effect of total body irradiation.     

Long-term in vivo left ventricular assist device study with a titanium centrifugal pump

A totally implantable centrifugal artificial heart has been developed. The plastic prototype, Gyro PI 601, passed 2 day hemodynamic tests as a functional total artificial heart, 2 week screening tests for antithrombogenicity, and 1 month system feasibility. Based on these results, a metallic prototype, Gyro PI 702, was subjected to in vivo left ventricular assist device (LVAD) studies. The pump system employed the Gyro PI 702, which has the same inner dimensions and the same characteristics as the Gyro PI 601, including an eccentric inlet port, a double pivot bearing system, and a magnet coupling system. The PI 702 is driven with the Vienna DC brushless motor actuator. For the in vivo LVAD study, the pump actuator package was implanted in the preperitoneal space in two calves, from the left ventricular apex to the descending aorta. Case 1 achieved greater than 9 month survival without any complications, at an average flow rate of 6.6 L/min with 10.2 W input power. Case 2 was killed early due to the excessive growth of the calf, which caused functional obstruction of the inlet port. There was no blood clot inside the pump. During these periods, neither case exhibited any physiologic abnormalities. The PI 702 pump gives excellent results as a long-term implantable LVAD.     

Somatostatin-receptor-negative carcinoid tumour responsible for Cushing''s syndrome

A 64-year-old woman presented with cardiomegaly, Sweet's syndrome, and refractory anemia (RA), and died of sudden cardiac arrest. The autopsy revealed a perivascular and myocardial infiltration by neutrophils, which could be responsible for the cardiomegaly and probably had caused disturbances in the conduction system leading to sudden cardiac arrest. Myocardial infiltration by functionally defective neutrophils can develop in a patient with myelodysplastic syndrome (MDS) without peripheral neutrophilia or leukemic blood picture and needs a special diagnostic and therapeutic consideration.     

Host utilization by the salmon louse Lepeophtheirus salmonis (Copepoda: Caligidae) in the Sea of Japan

Complex dynamic properties of a bolus in the mouth are specified by touch. Cooperation between touch and taste may help to specify the character of the bolus. The role of touch in specifying the presence of the oral bolus, in contrast to it's characteristics, remains a theoretical and experimental challenge.     

Nordihydroguaiaretic acid blocks protein transport in the secretory pathway causing redistribution of Golgi proteins into the endoplasmic reticulum

We have investigated the effect of nordihydroguaiaretic acid (NDGA), an inhibitor of lipoxygenase, on the intracellular protein transport and the structure of the Golgi complex. Pulse-chase experiments and immunoelectron microscopy showed that NDGA strongly inhibits the transport of newly synthesized secretory proteins to the Golgi complex resulting in their accumulation in the endoplasmic reticulum (ER). Despite their retention in the ER, oligosaccharides of secretory and ER-resident proteins were processed to endoglycosidase H-resistant forms, raising the possibility that oligosaccharide-processing enzymes are redistributed from the Golgi to the ER. Morphological observations further revealed that alpha-mannosidase II (a cis/medial-Golgi marker), but not TGN38 (a trans-Golgi network marker), rapidly redistributes to the ER in the presence of NDGA, resulting in the disappearance of the characteristic Golgi structure. Upon removal of the drug, the Golgi complex was reassembled into the normal structure as judged by perinuclear staining of alpha-mannosidase II and by restoration of the secretion. These effects of NDGA are quite similar to those of brefeldin A. However, unlike brefeldin A, NDGA did not cause a dissociation of beta-coatomer protein, a subunit of coatomer, from the Golgi membrane. On the contrary, NDGA exerted the stabilizing effect on beta-coatomer protein/membrane interaction against the dissociation caused by brefeldin A and ATP depletion. Taken together, these results indicate that NDGA is a potent agent disrupting the structure and function of the Golgi complex with a mechanism different from those known for other drugs reported so far.     

Nonuniform azimuth image shift observed in the Radarsat images of ships in motion

This paper describes, for the first time to the authors' knowledge, the phenomenon of nonuniform azimuth image shift of a rigid body observed in the Radarsat synthetic aperture radar (SAR) images of cruising ships. The effect is caused by the different slant-range velocities of coherent scatterers across the hull associated with the ship motions. The slant-range velocity is estimated from the SAR image of an identified ship, and it is compared with that computed from a numerical model using the ship's specification and meteorological data. The result indicates that the dominant contribution to the nonuniform image shift is the pitching motion of the ship. Further comparison is made with the wave orbital velocity, and the results are shown to be in good agreement. Comparisons are also made between the SAR-derived slant-range velocities of two unknown ships and wave orbital velocities, and reasonable agreement is obtained. One of the ships' images exhibits not only nonuniform shift but also image skew. The latter skewing effect may be caused by rolling of the ship.