Immunophenotypic analysis of peripheral blood mononuclear cells undergoing in vitro apoptosis after isolation from human immunodeficiency virus-infected children

Lymphocytes of human immunodeficiency virus (HIV)-infected individuals undergo accelerated apoptosis in vitro, but the subsets of cells affected have not been clearly defined. This study examined the relationship between lymphocyte phenotype and apoptotic cell death in HIV-infected children by flow cytometry. Direct examination of the phenotype of apoptotic lymphocytes was accomplished using a combination of surface antigen labeling performed simultaneously with the Tdt mediated Utp nick end-labeling (TUNEL) assay. In comparison to live cells, apoptotic lymphocytes displayed an overrepresentation of CD45RO and HLA-DR expressing cells, while CD28 and CD95 expressing cells were underrepresented. Lymphocytes expressing CD4, CD8, and CD38 were equally represented in apoptotic and live populations. When percent lymphocyte apoptosis follow- ing culture was examined independently with lymphocyte subsets in fresh blood, apoptosis was negatively correlated with the percentage of CD4 cells, but not with specific CD4 T-cell subsets. Although not correlated with the percentage of total CD8 cells, apoptosis was positively correlated with specific CD8 T-cell subsets expressing CD45RO and CD95 and negatively correlated for CD8 T cells expressing CD45RA. These results provide direct evidence that a population of activated lymphocytes with the memory phenotype lacking the costimulatory molecule CD28 are especially prone to undergo apoptosis. The findings related to CD95 expression in fresh and apoptotic cells implicate Fas-dependent and Fas-independent pathways of apoptosis in HIV disease in children.     

Design,Synthesis, and Characterization of Macrocyclic Inhibitors of the Proprotein Convertase Furin

The activation of viral glycoproteins by the host protease furin is an essential step in the replication of numerous pathogenic viruses. Thus, effective inhibitors of furin could serve as broad-spectrum antiviral drugs. A crystal structure of an inhibitory hexapeptide derivative in complex with furin served as template for the rational design of various types of new cyclic inhibitors. Most of the prepared derivatives are relatively potent furin inhibitors with inhibition constants in the low nanomolar or even sub-nanomolar range. For seven derivatives the crystal structures in complex with furin could be determined. In three complexes, electron density was found for the entire inhibitor. In the other cases the structures could be determined only for the P6/P5-P1 segments, which directly interact with furin. The cyclic derivatives together with two non-cyclic reference compounds were tested as inhibitors of the proteolytic activation and replication of respiratory syncytial virus in cells. Significant antiviral activity was found for both linear reference inhibitors, whereas a negligible efficacy was determined for the cyclic derivatives.     

Lancing Drug Reservoirs into Subcutaneous Fat to Combat Obesity and Associated Metabolic Diseases

Obesity is a serious epidemic health problem that can cause many other diseases including type 2 diabetes and cardiovascular diseases. Current approaches to combat obesity suffer from low effectiveness and adverse side effects. Here, a new self‐administrable and minimally invasive transdermal drug delivery strategy for home‐based long‐term treatment of obesity and other diseases is developed. Specifically, ultrathin, core‐shelled, and lance‐shaped polymeric drug reservoirs (micro‐lances [MLs]) are readily fabricated by a thermal pressing molding method and totally implanted into subcutaneous fat by lancing through the skin. Using a diet‐induced obese mouse model, it is shown that the development of obesity and associated metabolic disorders is effectively inhibited by applying therapeutic core‐shelled MLs once every 2 weeks. The outstanding therapeutic effects are attributable to highly localized and biphasic drug release, as well as combination therapy based on browning transformation of white fat and enhanced insulin sensitivity.     

从位置参数入手直接建立圆点曲线方程

根据刚体四个有限位置的位移参数及点四位置共圆的等圆周角条件，列出了复极矢量等式．在刚体坐标系上，直接导出了圆点曲线方程，并给出圆点坐标的具体算法．     

A review of localization systems for robotic endoscopic capsules

Obscure gastrointestinal (GI) bleeding, Crohn disease, Celiac disease, small bower tumors, and other disorders that occur in the GI tract have always been challenging to be diagnosed and treated due to the inevitable difficulty in accessing such a complex environment within the human body. With the invention of wireless capsule endoscope, the next generation of the traditional cabled endoscope, not only a dream has come true for the patients who have experienced a great discomfort and unpleasantness caused by the conventional endoscopic method, but also a new research field has been opened to develop a complete miniature robotic device that is swallowable and has full functions of diagnosis and treatment of the GI diseases. However, such an ideal device needs to be equipped with a highly accurate localization system to be able to exactly determine the location of lesions in the GI tract and provide essential feedback to an actuation mechanism controlling the device's movement. This paper presents a comprehensive overview of the localization systems for robotic endoscopic capsules, for which the motivation, challenges, and possible solutions of the proposed localization methods are also discussed.     

Potentialities of Renewable Energy in Victoria,Australia

In utility power system, electricity demand is being covered largely by fossil fueled power generation, which contributes high level of GHG （greenhouse gas） emission and causes global warming worldwide. In order to reduce GHG emission level, most of the countries in the world targeting towards green energy that is power generation from RE （renewable energy） sources. In this paper, it is considered to study prospects of RE sources in particular, solar and wind in Victoria State which are abundant as compared to other sources of renewable. The wind and solar energy feasibility study and sensitivity analysis has been done for Victoria with the aid of HOMER （hybrid optimization model of electric renewable） simulation software. From the study, it has clearly evicted that wind energy combinational HPS （hybrid power system） has more contribution, and high potential than solar PV （photovoltaic） systems for a particular location. This study also investigates the influences of energy storage in the proposed HPS.     

岩爆模拟材料的筛选试验研究

根据岩爆模拟试验的要求,进行了相似材料的配合比试验,筛选出若干适合作为岩爆模型的相似材料。试验过程中就试验设备、材料配合比、模型制作、典型试验等方面进行了有益的探讨。     

An effective and interpretable method for document classification

As the number of documents has been rapidly increasing in recent time, automatic text categorization is becoming a more important and fundamental task in information retrieval and text mining. Accuracy and interpretability are two important aspects of a text classifier. While the accuracy of a classifier measures the ability to correctly classify unseen data, interpretability is the ability of the classifier to be understood by humans and provide reasons why each data instance is assigned to a label. This paper proposes an interpretable classification method by exploiting the Dirichlet process mixture model of von Mises–Fisher distributions for directional data. By using the labeled information of the training data explicitly and determining automatically the number of topics for each class, the learned topics are coherent, relevant and discriminative. They help interpret as well as distinguish classes. Our experimental results showed the advantages of our approach in terms of separability, interpretability and effectiveness in classification task of datasets with high dimension and complex distribution. Our method is highly competitive with state-of-the-art approaches.     

Differential Cytotoxicity of Curcumin-Loaded Micelles on Human Tumor and Stromal Cells

Although curcumin in the form of nanoparticles has been demonstrated as a potential anti-tumor compound, the impact of curcumin and nanocurcumin in vitro on normal cells and in vivo in animal models is largely unknown. This study evaluated the toxicity of curcumin-loaded micelles in vitro and in vivo on several tumor cell lines, primary stromal cells, and zebrafish embryos. Breast tumor cell line (MCF7) and stromal cells (human umbilical cord vein endothelial cells, human fibroblasts, and human umbilical cord-derived mesenchymal stem cells) were used in this study. A zebrafish embryotoxicity (FET) assay was conducted following the Organisation for Economic Co-operation and Development (OECD) Test 236. Compared to free curcumin, curcumin PM showed higher cytotoxicity to MCF7 cells in both monolayer culture and multicellular tumor spheroids. The curcumin-loaded micelles efficiently penetrated the MCF7 spheroids and induced apoptosis. The nanocurcumin reduced the viability and disturbed the function of stromal cells by suppressing cell migration and tube formation. The micelles demonstrated toxicity to the development of zebrafish embryos. Curcumin-loaded micelles demonstrated toxicity to both tumor and normal primary stromal cells and zebrafish embryos, indicating that the use of nanocurcumin in cancer treatment should be carefully investigated and controlled.     

Operation and Control of a Full-Scale Biogas Plant Treating Wastewater from the Cleaning of Car Tanks

A 1200-m³ full-scale biogas plant treating highly polluted wastewater (WW) from the cleaning of tank cars transporting food and fodder is working stably and efficiently despite the WW is constantly varying in strength and composition, is easily acidifying, and low in alkalinity. Na₂CO₃ has to be added to maintain the digester alkalinity and the digester pH at defined values due to softened water used for car tank cleaning. Monitoring volatile organic acid concentration and controlling the alkalinity in the digester are essential for maintaining the digestion process stability. Biogas production is adapted to the demand by a modulated feeding regime. The drastic modulation of feeding is possible due to the moderate organic loading of the plant along with the high average hydraulic retention time.