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51.
We have used a diagrammatic approach first developed for nonlinear optical calculations of the density operator to obtain expressions for the transition probability in various laser-switched collision processes. Not only do these diagrams considerably simplify the algebra involved in such calculations, but they also afford a clear visualization of the interaction. In particular, pair absorption, laser-switched Raman scattering, and collisionally enhanced hot luminescence and four-wave mixing processes are considered. 相似文献
52.
Protein sizing on a microchip 总被引:6,自引:0,他引:6
We have developed a microfabricated analytical device on a glass chip that performs a protein sizing assay, by integrating the required separation, staining, virtual destaining, and detection steps. To obtain a universal noncovalent fluorescent labeling method, we have combined on-chip dye staining with a novel electrophoretic dilution step. Denatured protein-sodium dodecyl sulfate (SDS) complexes are loaded on a chip and bind a fluorescent dye as the separation begins. At the end of the separation channel, an intersection is used to dilute the SDS below its critical micelle concentration before the detection point. This strongly reduces the background due to dye molecules bound to SDS micelles and also increases the peak amplitude by 1 order of magnitude. Both the on-chip staining and SDS dilution steps occur in the 100-ms time scale and are approximately 10(4) times faster than their conventional counterparts in SDS-PAGE. This represents a much greater speed increase due to microfabrication than has been obtained in other assay steps such as electrophoretic separations. We have designed and tested a microchip capable of sequentially analyzing 11 different samples, with sizing accuracy better than 5% and high sensitivity (30 nM for carbonic anhydrase). 相似文献
53.
Singer Philipp; Boison Detlev; M?hler Hanns; Feldon Joram; Yee Benjamin K. 《Canadian Metallurgical Quarterly》2007,121(5):815
Selective deletion of glycine transporter 1 (GlyT1) in forebrain neurons enhances N-methyl-D-aspartate receptor (NMDAR)-dependent neurotransmission and facilitates associative learning. These effects are attributable to increases in extracellular glycine availability in forebrain neurons due to reduced glycine re-uptake. Using a forebrain- and neuron-specific GlyT1-knockout mouse line (CamKIIαCre; GlyT1tm1.2fl/fI), the authors investigated whether this molecular intervention can affect recognition memory. In a spontaneous object recognition memory test, enhanced preference for a novel object was demonstrated in mutant mice relative to littermate control subjects at a retention interval of 2 hr, but not at 2 min. Furthermore, mutants were responsive to a switch in the relative spatial positions of objects, whereas control subjects were not. These potential procognitive effects were demonstrated against a lack of difference in contextual novelty detection: Mutant and control subjects showed equivalent preference for a novel over a familiar context. Results therefore extend the possible range of potential promnesic effects of specific forebrain neuronal GlyT1 deletion from associative learning to recognition memory and further support the possibility that mnemonic functions can be enhanced by reducing GlyT1 function. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
54.
Tso Yee Fan Ripin D.J. Aggarwal R.L. Ochoa J.R. Bien Chann Tilleman M. Spitzberg J. 《IEEE journal of selected topics in quantum electronics》2007,13(3):448-459
Cryogenically cooled solid-state lasers promise a revolution in power scalability while maintaining a good beam quality because of significant improvements in efficiency and thermo-optic properties. This is particularly true for Yb lasers because of their relatively low quantum defect and relatively broadband absorption even at cryogenic temperatures. Thermo-optic properties of host materials, including thermal conductivity, thermal expansion, and refractive index at low temperature, are reviewed and data presented for YAG (ceramic and single crystal), GGG, GdVO4, and Y2O3. Spectroscopic properties of Yb:YAG and Yb:LiYF4 (YLF) including absorption cross sections, emission cross sections, and fluorescence lifetimes at cryogenic temperatures are characterized. Recent experiments have pushed the power from an end-pumped cryogenically cooled Yb:YAG laser to 455-W continuous-wave output power from 640-W incident pump power at an of M2 1.4. 相似文献
55.
James Tow Renato N. Gadenz 《International Journal of Circuit Theory and Applications》1978,6(2):147-152
Levi's and Zeheb's approach for approximating a high-Q second-order section by n sections with lower Q's is compared with the optimal solution of this problem proposed by Khazanov. Tables relating the maximum error in the approximation to the values of n and the achievable Q-reduction (QR) are also given. They should prove useful to the designer. Finally, some comments on sensitivities are added. 相似文献
56.
Jose G. Guarnizo Martin Mellado Cheng Yee Low Francisco Blanes 《Advanced Robotics》2013,27(15):989-1004
Coordination strategy is a relevant topic in multi-robot systems, and robot soccer offers a suitable domain to conduct research in multi-robot coordination. Team strategy collects and uses environmental information to derive optimal team reactions, through cooperation among individual soccer robots. This paper presents a diagrammatic approach to architecting the coordination strategy of robot soccer teams by means of a principle solution. The proposed model focuses on robot soccer leagues that possess a central decision-making system, involving the dynamic selection of the roles and behaviors of the robot soccer players. The work sets out from the conceptual design phase, facilitating cross-domain development efforts, where different layers must be interconnected and coordinated to perform multiple tasks. The principle solution allows for intuitive design and the modeling of team strategies in a highly complex robot soccer environment with changing game conditions. Furthermore, such an approach enables systematic realization of collaborative behaviors among the teammates. 相似文献
57.
Heald R. Aingaran K. Amir C. Ang M. Boland M. Dixit P. Gouldsberry G. Greenley D. Grinberg J. Hart J. Horel T. Wen-Jay Hsu Kaku J. Chin Kim Song Kim Klass F. Kwan H. Lauterbach G. Lo R. McIntyre H. Mehta A. Murata D. Nguyen S. Yet-Ping Pai Patel S. Shin K. Tam K. Vishwanthaiah S. Wu J. Yee G. You E. 《Solid-State Circuits, IEEE Journal of》2000,35(11):1526-1538
This quad-issue processor achieves 1-GHz operation through improved dynamic circuit techniques in critical paths and a more extensive on-chip memory system which scales in both bandwidth and latency. Critical logic paths use domino, delayed clocked domino, and logic embedded in dynamic flip-flops for minimum delay. A 64-KB sum-addressed memory data cache combines the address offset add with the cache decode, allowing the average memory latency to scale by more than the clock ratio. Memory bandwidth is improved by using wave pipelined SRAM designs for on-chip caches and a write cache for store traffic. Memory power is controlled without increased latency by use of delayed-reset logic decoders. The chip operates at 1000 MHz and dissipates less than 80 W from a 1.6-V supply. It contains 23 million transistors (12 million in RAM cells) on a 244 mm2 die 相似文献
58.
59.
Single, unreliable machine producing n part types is considered. Using a continuous flow model, conditions are found for the optimality of a just-in-time, or zero-inventory, policy via a stochastic coupling analysis. These conditions are proven to be necessary and conjectured to be sufficient for optimality. This correspondence extends the results of Veatch and Caramanis, in which necessary and sufficient conditions are found for a single machine producing two part types, and it strengthens the results of Perkins and Srikant, in which sufficient conditions are found for n part types 相似文献
60.
Csomor Philipp A.; Yee Benjamin K.; Vollenweider Franz X.; Feldon Joram; Nicolet Tiana; Quednow Boris B. 《Canadian Metallurgical Quarterly》2008,122(4):885
Prepulse inhibition (PPI) of the startle reflex refers to the reduction of the reflexive startle response to an intense pulse stimulus when its presentation is shortly preceded by a weak prepulse stimulus. PPI is considered as a cross-species translational model of sensorimotor gating, and deficient PPI has been reported in a number of neuropsychiatric disorders. Although a part of the literature is based on the assumption that PPI is independent of the baseline startle reaction, there is accumulating evidence (Csomor et al., 2006; Sandner & Canal, 2007; Yee, Chang, Pietropaolo, & Feldon, 2005) that argues against such an independency. The authors systematically investigated whether PPI indexed as percentage or difference score is dependent on the magnitude of baseline startle reactivity in healthy human volunteers and in C57BL/6 mice. The results revealed that both indexations of PPI were affected by the magnitude of the baseline startle. The authors highlight the pitfalls of different methods to index PPI, especially when startle reactivity differs considerably between groups under comparison, and offer practical recommendations to satisfactorily deal with such baseline differences. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献