Reducing Salmonella on cantaloupes and honeydew melons using wash practices applicable to postharvest handling, foodservice, and consumer preparation

Washing conditions that included a soak or brush scrub were evaluated for removal of Salmonella from the surface of smooth (honeydew) or complex (cantaloupe) melon rinds. Melon rinds were spot-inoculated onto a 2.5 cm2 area of rind (squares) with approximately 6.0 log(10) CFU/square of an avirulent nalidixic acid-resistant strain of Salmonella typhimurium. Melons were washed by immersion in 1500 ml of water or 200 ppm total chlorine and allowed to soak or were scrubbed over the entire melon surface with a sterile vegetable brush for 60 s. Inoculated sites, uninoculated sites ("next to" sites) that were adjacent to inoculated sites, and sites on the side of the melon opposite (remote sites) the inoculated site were excised and pummeled in a stomacher for 2 min prior to plating onto tryptic soy or bismuth sulfite agar supplemented with 50 microg/ml nalidixic acid. S. typhimurium was reduced on the rind of cantaloupe by 1.8 log CFU/melon after soaking for 60 s in 200 ppm total chlorine, which was significantly better than the 0.7 log CFU/melon achieved with soaking in water. For both water and 200 ppm total chlorine, scrubbing with a vegetable brush was shown to be significantly (0.9 log CFU/cantaloupe) more effective than soaking alone. When honeydew melons were soaked or scrubbed in water, reductions of 2.8 log CFU/melon or >4.6 log CFU/melon (four of five samples), respectively, were observed. However, when water treatments were used, the presence of Salmonella-positive "next to" and remote sites indicated that bacteria were spread from inoculated site on the rind to uninoculated sites either through the rinse water (40-70 CFU/ml of Salmonella) or scrub brush (400-500 CFU/brush). Transfer to other sites occurred more often with cantaloupe than honeydew melons. This transfer was eliminated when 200 ppm total chlorine was used. When 200 ppm total chlorine was used, Salmonella could not be detected in the water or on the scrub brush. For optimal microbial removal in food service and home settings, melons should be scrubbed with a clean brush under running water. However, to ensure the benefits of brushing, instructions for cleaning and sanitizing brushes must also be emphasized. For food service settings where concentration and pH can be adequately measured, the use of chlorinated water may provide additional benefit.     

Effect of water immersion on the microtopography of the skin of chicken carcasses

Water-immersion cleaning and chilling of poultry carcasses caused the skin to swell and exposed deep channels and crevices in the skin surface as a result of water uptake. These changes were demonstrated by electron microscopy and were shown to be dependent on the time of immersion and the temperature of the water. Addition of sodium chloride to the immersion medium and manipulation of the medium pH did not markedly affect or prevent these changes. The implications of these changes for microbial contamination during water-immersion chilling are discussed.     

Durability issues and service lifetime prediction of electrochromic windows for buildings applications

In general, the purposes of this paper are to elucidate the crucial importance of durability and service lifetime prediction (SLP) for electrochromic windows (ECWs) and to present an outline for developing a SLP methodology for ECWs. The specific objectives are (a) to illustrate the generic nature of SLP for several types of solar energy conversion or energy conservation devices, (b) to summarize the major durability issues associated with ECWs, (c) to justify using SLP in the triad of cost, performance, and durability rather than just durability, (d) to define and explain the seven major elements that constitute a generic SLP methodology, (e) to provide background for implementing the SLP methodology for ECWs, including the complexity of the potential degradation mechanisms, and (f) to provide an outline of studies using ECWs for improving the durability of ECW materials and predicting a service lifetime for ECWs using the SLP methodology outlined in objective (d). Our major conclusions are that substantial R&Dis necessary to understand the factors that limit ECW durability, and that it is possible to predict the service lifetime of ECWs.     

Phonological universals constrain the processing of nonspeech stimuli.

Domain-specific systems are hypothetically specialized with respect to the outputs they compute and the inputs they allow (Fodor, 1983). Here, we examine whether these 2 conditions for specialization are dissociable. An initial experiment suggests that English speakers could extend a putatively universal phonological restriction to inputs identified as nonspeech. A subsequent comparison of English and Russian participants indicates that the processing of nonspeech inputs is modulated by linguistic experience. Striking, qualitative differences between English and Russian participants suggest that they rely on linguistic principles, both universal and language-particular, rather than generic auditory processing strategies. Thus, the computation of idiosyncratic linguistic outputs is apparently not restricted to speech inputs. This conclusion presents various challenges to both domain-specific and domain-general accounts of cognition. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Universal constraints on the sound structure of language: Phonological or acoustic?

Languages are known to exhibit universal restrictions on sound structure. The source of such restrictions, however, is contentious: Do they reflect abstract phonological knowledge, or properties of linguistic experience and auditory perception? We address this question by investigating the restrictions on onset structure. Across languages, onsets of small sonority distances are dispreferred (e.g., lb is dispreferred to bn). Previous research with aural materials demonstrates such preferences modulate the perception of unattested onsets by English speakers: Universally ill-formed onsets are systematically misperceived (e.g., lba → leba) relative to well-formed onsets (e.g., bn). Here, we show that the difficulty to process universally ill-formed onsets extends to printed materials. Auxiliary tests indicate that such difficulties reflect phonological, rather than orthographic knowledge, and regression analyses demonstrate such knowledge goes beyond the statistical properties of the lexicon. These findings suggest that speakers have abstract, possibly universal, phonological knowledge that is general with respect to input modality. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Poly (3,4-ethylenedioxythiophene) for chronic neural stimulation.

Chronic neural stimulation using microelectrode arrays requires highly stable and biocompatible electrode materials with high charge injection capability. Conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was electrochemically deposited on thin film Pt electrodes of stimulation electrode arrays to evaluate its properties for chronic stimulation. The coated electrodes demonstrated much lower impedance than thin film Pt due to the high surface area and high ion conductivity across the film. The PEDOT film also presents intrinsic redox activity which contributes to the low impedance as well as a much higher charge storage capacity. The charge injection limit of PEDOT electrode was found to be 2.3 mC/cm2, comparable to IrOx and much higher than thin film Pt. Under biphasic stimulation, the coated electrodes exhibited lower voltage and linear voltage excursion. Well-coated PEDOT electrodes were stable under chronic stimulation conditions, suggesting that PEDOT is a promising electrode material to be further developed for chronic neural stimulation applications.     

Cell migration and proliferation during monolayer formation and wound healing

The interaction of living cells with surfaces is important in applications of biomaterials, such as tissue engineering. Characterising and modelling the attachment, migration and proliferation of cells on materials used for tissue engineering provides valuable insight into their potential applications as well as a means of objective comparison. In this study, proliferation and migration of NIH-3T3 fibroblast cells on tissue culture plastic in vitro were quantified. The development of randomly scattered individual cells into confluent cell monolayers proceeded with cell density following a logistic growth pattern. Travelling cell wavefronts produced in a wound healing assay were modelled with a modified Fisher equation incorporating both diffusion and logistic growth. The diffusivity and growth rates thus determined could be used for comparison with cell behaviour on other surfaces or under different conditions. Cell tracking showed that the average effective velocity of cells varied inversely with cell density, supporting contact inhibition of cell movement.     

Stress-strain response of a cast 319-T6 aluminum under thermomechanical loading

The stress-strain behavior of cast 319-T6 aluminum-copper alloys with three different secondary dendrite arm spacings (SDASs) was studied at high temperatures and under thermomechanical deformation, exposing marked cyclic softening. A two state-variable unified inelastic constitutive model proposed earlier was modified to describe the stress-strain responses of these alloys by considering the variation of hardening and recovery functions of back-stress and drag stress. The SDAS was incorporated in the model as a length-scale parameter, and the material constants were determined systematically from experiments on a cast 319-T6 aluminum with small and large SDASs. The capabilities of the constitutive model were checked by the comparisons of simulations to experiments in the small-strain regime (<0.005). The results show that the model provides successful simulations for material response after thermal exposure at high temperature and cyclic transient stress-strain behavior. The causes of mechanical behaviors at the macro scale are discussed based on microstructural changes during thermal exposure.