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Using CD spectroscopy, guanine tetraplex formation was studied with short DNA fragments in which cytosine residues were systematically added to runs of guanine either at the 5' or 3' ends. Potassium cations induced the G-tetraplex more easily with fragments having the guanine run at the 5' end, which is just an opposite tendency to what was reported for (G+T) oligonucleotides. However, the present (G+C) fragments simultaneously adopted other conformers that complicated the analysis. We demonstrate that repeated freezing/thawing, performed at low ionic strength, is a suitable method to exclusively stabilize the tetraplex in the (G+C) DNA fragments. In contrast to KCl, the repeated freeze/thaw cycles better stabilized the tetraplex with fragments having the guanine run on the 3' end. The tendency of guanine blocks to generate the tetraplex destabilized the d(G5).d(C5) duplex whose strands dissociated, giving rise to a stable tetraplex of (dG5) and single-stranded (dC5). In contrast to d(G3C3) and d(G5C5), repeated freezing/thawing induced the tetraplex even with the self-complementary d(C3G3) or d(C5G5); hence the latter oligonucleotides preferred the tetraplex to the apparently very stable duplex. The tetraplexes only included guanine blocks while the 5' end cytosines interfered neither with the tetraplex formation nor the tetraplex structure. 相似文献
53.
文章采用系统工程的观点和方法来讨论计算机这一现代化信息处理工具如何在市场营销管理中的应用问题。首先分析了市场营销管理系统的现状和存在的问题、系统的输入输出信息关系以及系统的数据流程图(即业务流程图)等,然后根据现代化市场营销管理的要求,构造出市场营销管理系统的功能结构图和系统的计算机处理模型图(即系统模型图)。 相似文献
54.
Byssochlamys fulva, isolated from corn, was grown on nutrient-amended shredded wheat medium for 14 days at 25 C. Crude solvent extract from these cultures was toxic to brine shrimp, chicken embryos, and rats. The extract was slightly inhibitory to the germination of of pea seeds, but was nontoxic to ten species of bacteria and one of yeast. One metabolite was isolated, given the trivial name byssotoxin A, and partially characterized chemically and physically. 相似文献
55.
随着人们对环境的日益关注,制浆造纸工业漂白废水的排放也受到了更加严格的限制.因此,降低纸浆漂白对环境的影响就显得尤为重要.使用生物制剂(如半纤维素酶)是降低耗氯量的方法之一,Viikari等人早在1986年就首次验证了这个观点.据报道,半纤维素酶对阔叶木浆和针叶木浆的作用效果不同. 相似文献
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Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods
Einar Ringø Renate Andersen Sigmund Sperstad Zhigang Zhou Pen Ren Eva Marie Breines 《Food Biotechnology》2014,28(4):333-353
The bacterial community of fermented horse milk (koumiss) from Mongolia was studied using three methods: cultivation, direct identification by 16S rRNA clone library and denaturing gradient gel electrophoresis (DGGE). Ninety-eight strains were isolated by traditional cultivation and 61 of those were randomly selected for further identification by 16S rRNA gene sequencing. The strains were dominated by lactic acid bacteria (LAB; six different lactobacilli), Acinetobacter, Bacillus and Psychrobacter. Construction of the clone library analysis revealed that 16S sequences of 220 clones, genus Lactobacillus was dominant, but Streptococcus thermophilus, Acetobacter pasteurianus and uncultured clones were also detected. Ten unique bands were sequenced from the DGGE and revealed: Lactococcus lactis, Lactococcus lactis subsp. lactis, Clostridium acidurici, Acinetobacter johnsonii, Dickeya sp., Enterobacter sp., Pseudomonas sp., Raoultella sp., and Ruminococcus sp.. In vitro growth inhibition of three human pathogens, Escherichia coli, Enterobacter sakazakii and Staphylococcus aureus by 14 culturable bacteria displayed that only three of the isolates tested inhibit growth of E. sakazakii while most of the other bacteria delayed growth of the target bacteria. 相似文献
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单压区靴式压榨的发展进一步改善了纸机的生产能力和效率。这种新技术已成功地应用于斯洛伐克的1台高速复印纸机上。压榨后的纸幅干度很高,松厚度好,并且两面差小。 相似文献
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