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31.
Electron spectroscopic imaging (ESI) was used to analyze the element content of melanin granules in the stria vascularis seen in ultrathin sections of Spurr-embedded cochleae of the guinea pig. To determine element composition, ESI images were taken at different ionization edges, and non-specific background signals were subtracted digitally by an image processing system. The presence of calcium and nitrogen in the melanin granules could be demonstrated clearly. The calcium identified in the melanin granules was then compared with the spatial distributions of calcium binding sites after the application of an antimonate precipitation method, which was used to localize loosely bound calcium. Despite a high calcium concentration within the granules, only very small single scattered calcium precipitates could be detected between these structures as compared with the amount of calcium precipitates attached to the plasma membrane or located within the cell nuclei. The nearly complete absence of precipitates within the melanin granules after the application of antimonate suggests differences in calcium binding and mobility involved in various physiological processes of ion balance regulation within the stria vascularis. 相似文献
32.
A Witschi L Kr?henbühl E Frei J Saltzman PJ Sp?th UR Müller 《Canadian Metallurgical Quarterly》1996,111(1):96-98
One case of prenatally diagnosed congenital diaphragmatic hernia was reviewed in terms of the anesthetic managements. Concentrations of diazepam, pancuronium and fentanyl were measured in maternal, fetal and umbilical serum. As expectedly, the transition of diazepam through the placenta was large and the concentration of diazepam in the fetal serum was equal to that of maternal serum, but only a small amount of pancuronium was transferred. 相似文献
33.
UR Hengge NH Brockmeyer R Malessa U Ravens M Goos 《Canadian Metallurgical Quarterly》1993,37(5):1010-1014
Hypertension is a major complication of rHuEPO therapy in hemodialysis (HD) patients. We have previously reported that patients receiving rHuEPO intravenously (i.v.) had higher mean arterial pressure (MAP) and plasma endothelin-1 (ET-1) levels than those in which the hormone was administered subcutaneously (s.c.). To test whether the increased serum ET-1 levels associated with i.v. rHuEPO administration are the result of a direct effect of the hormone on ET-1 release by the endothelial cells (EC), we examined the effects of rHuEPO in vitro. Bovine pulmonary artery endothelial cells (BPAEC) were exposed to doses of rHuEPO of 0.8; 1.6; 3.3 and 6.6 U/ml. A 24 hour-time course showed maximal ET-1 production at 12 hours for all the doses tested. A significant increase in cell proliferation over controls was observed at 24 hours, for all rHuEPO doses, and no correlation was found between ET-1 values and cell proliferation. Inhibition of protein synthesis by cycloheximide (10 micrograms/ml) abolished the stimulation of ET-1 release by rHuEPO. Thrombin (4 U/ml) and angiotensin II (10(-7) M), two potent stimulators of ET-1 release, had additive effects to those of rHuEPO. Specific thrombin and angiotensin II antagonists blocked these additive effects, reducing ET-1 release to the level of rHuEPO stimulation alone. In summary, rHuEPO stimulates vascular EC in culture to increase ET-1 release through an increase in synthesis and in a time dependent fashion. The routes of stimulation seem to differ from other known ET-1 secretogoges. Our data also confirm a significant mitogenic effect of rHuEPO on the endothelial cell. 相似文献
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Hans Schirmer 《Energy and Buildings》1984,7(1):35-53
One of the most important tasks of regional land-use planning is to protect the natural resources. Damage and prejudices caused by planning activities can either be reduced or prevented by considering the interactions in the biosphere. The climatologist acting as advisor, has to be prepared for the relevant project, especially with the large-scale maps. For this purpose, however, the available climatological basic material does not suffice; consequently it has to be essentially supplemented by acquiring additional data and developing new methods. The general climatic structure of the area or country in question plays a decisive role, so methods as well as analytic and synthetic results are not transferable to other areas or countries in every case. Another aspect is that often different planning purposes have to be considered because of the differences in the infrastructure of the corresponding area.Based on case studies, the planning purposes are presented, in particular for human settlements, trade and industry, and results are given of the pertaining climatological investigations, partly with an evaluation of the thermal and air-hygienic discomfort. The large economic benefit which can be gained by this special field of applied climatology shall serve as an example for the great importance of the World Climate Applications Programme (WCAP) within the framework of the World Climate Programme (WCP) which has already been started. 相似文献
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A detector for liquid chromatography, based on anodic electrochemical oxidation at a glassy carbon electrode, is used in combination with reversed-phase liquid chromatography for the determination of the long-acting tricyclic psychotropic drugs fluphenazine and perphenazine in blood. Completely porous microparticulate silica material, modified so as to give a methyl silica, was used as the stationary phase and buffered mixtures of methanol and water as the mobile phase. The low detection limit and the selectivity of the coulometric detector made possible the determination of concentrations of 1-20 ppb of the compounds of interest. An extraction procedure was in order to obtain sufficient selectivity. Data for the precision, detection limit and yield of the extraction procedure are given. A number of serum levels of perphenazine after oral administration and of fluphenazine after i.m. doses of fluphenazine decanoate are stated. 相似文献
39.
Holger Weiß Georg Teutsch Peter Fritz Birgit Daus Andreas Dahmke Peter Grathwohl Ralf Trabitzsch Bernd Feist Ralf Ruske Olaf Böhme Mario Schirmer 《Grundwasser》2001,6(3):113-122
The SAFIRA-project (remediation research in regionally contaminated aquifers) focuses on innovative in situ remediation technologies to treat complex groundwater contamination. For this purpose, a model site located in Bitterfeld, Germany, was selected. The site is heavily contaminated with aliphatic and aromatic chlorinated hydrocarbons. The goal of the project is to develop, test and optimize new technologies of reactive barriers under controlled in situ conditions in a pilot plant. In order to create the necessary basis for this model project, the hydrogeological and hydrogeochemical conditions at the selected field site were investigated and various technologies for the design of the in situ pilot plant were examinated. Small-scale laboratory studies and tests in a mobile test unit (scale 1 m) were carried out in order to test the technologies prior to their application in the field. The technologies now tested in the reactive columns at the pilot plant include physico-chemical, microbial and combined techniques. 相似文献
40.
Dipl.-Geol. A. Musolff Dipl.-Hydrol. S. Leschik Dr. F. Reinstorf Dr. G. Strauch Prof. Dr. habil. M. Schirmer Dr. M. Möder 《Grundwasser》2007,12(3):217-231
Xenobiotics are increasingly being considered as ecotoxicologically relevant for the aquatic environment and human health. Their behaviour and the effects on the environment have not yet been comprehensively investigated and, therefore, are currently the subject of the project WASSER Leipzig initiated by the UFZ. The results of this article are based on groundwater and surface water analyses of a watershed within the town of Leipzig. Here the industrial chemicals bisphenol-A and t-nonylphenol, the polycyclic fragrances galaxolide and tonalide, the antiepileptic drug carbamacepine and caffeine where investigated. Xenobiotics showed ubiquitous occurrence in the rivers, which were contaminated from treated and untreated sewage, as well as in groundwater, contaminated by leaky sewers. Mean concentrations up to several hundred ng/l were found in the rivers, while groundwater concentrations, except for bisphenol-A, tended to be lower. Applying the statistical factor analysis on the hydrochemical measurements, a differentiation of the xenobiotics with regard to their hydrochemical behaviour in groundwater was performed. 相似文献