Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250

Hapten refers to a chemical compound of small molar mass (typically less than 1000 daltons) that can bind with an antibody, but cannot initiate an immune response by itself unless it is conjugated to a protein carrier of larger molar mass. A novel method to prepare a hapten to generate anti-hapten immunity without covalent conjugation to a carrier was developed. Coating both water-soluble and -insoluble haptens onto a nitrocellulose membrane effectively presented haptens to the system and caused the generation of specific anti-hapten B lymphocytes and antibodies by immunization both in vitro and in vivo. This method has a potential to substitute for conventional hapten carrier conjugation to generate anti-hapten immunity.     

Spatiotemporal Modelling of Water Balance Components in Response to Climate and Landuse Changes in a Heterogeneous Mountainous Catchment

Landuse change and climate change are the main drivers of hydrological processes. The purpose of this study was to analyse the separate and combined future effects of climate and landuse changes on water balance components on different spatial and temporal scales using the integrated hydrological Soil and Water Assessment Tool model. The study focused on the changes and relationship between water yield (WYLD) and sediment yield (SYLD) in the heterogeneous Taleghan Catchment in Iran. For future climate scenarios, RCP 4.5 and RCP 8.5 of GFDL-ESM2M GCM were used for 2020–2040. A Markov chain model was used to predict landuse change in the catchment. The results indicated an increase in precipitation and evapotranspiration. The findings also showed that the relationship between WYLD and SYLD is direct and synergic. Climate change has a stronger effect on WYLD than landuse change, whereas landuse change has a stronger effect on SYLD. The conversion of rangelands to barren land is the most critical landuse change that could increase SYLD. The highest increase in WYLD and SYLD in scenario RCP4.5 resulted from the combined effects of climate and landuse change. We estimated WYLD of about 295 mm and SYLD of around 17 t/ha. The proposed methodology is universal and can be applied to similar settings to identify the most vulnerable regions. This can help prioritize management strategies to improve water and soil management in watersheds.

     

New computation method for stratification pipes of solar storage tanks

The efficiency of low-flow solar systems is strongly influenced by the quality of the thermal stratification in the storage tank. The better a thermal stratification can be generated and maintained, the higher can be the yield of the solar system. Fluid mechanical charge systems are often used for this purpose, which cause, however, undesirable sucking effects. Therefore, the knowledge of the appearing fluid flows as well as the knowledge of the consequences of constructive changes are very important for the design of such charge systems. However, simulations with CFD (Computational Fluid Dynamics) are very costly and time-consuming. In this article a new and much simpler computation method is introduced making the determination of the individual fluid flows and the estimation of the effects of constructive changes possible. The computations can be carried out within short time. The comparison with CFD gives a qualitatively good agreement for a simple charge system. The results of a constructive modification of the charge system reducing the sucking effect are discussed. The remaining quantitative differences result from the discrepancies between the non-ideal behaviour of the real fluid and the model assumptions and point out improvement potentials.     

MICROMACHINED PIEZOELECTRIC MICROSPEAKERS FABRICATED WITH HIGH QUALITY ALN THIN FILM

ABSTRACT

This paper describes the micromachined piezoelectric microspeakers that can produce the audible signal with 20 V peak-to-peak input voltages. The diaphragm size is 4 × 4 mm² and the thickness of diaphragm is around 1 micron meter except partially etched piezoelectric area. The maximum sound output pressure of the microspeaker is even higher than ever before with a small diaphragm in high frequency range around 10 kHz. This successful result bases upon using high quality AlN thin film. The deposited AlN thin film shows c-axis oriented columnar structure and very fine grains. The highest SPL (Sound Pressure Level) measured from 300 Hz to 12 kHz shows about 100 dB around 10 kHz in case of circular type microspeaker and about 76 dB in case of cross type, respectively.     

NOM degradation during river infiltration: Effects of the climate variables temperature and discharge

Most peri-alpine shallow aquifers fed by rivers are oxic and the drinking water derived by riverbank filtration is generally of excellent quality. However, observations during past heat waves suggest that water quality may be affected by climate change due to effects on redox processes such as aerobic respiration, denitrification, reductive dissolution of manganese(III/IV)- and iron(III)(hydr)oxides that occur during river infiltration. To assess the dependence of these redox processes on the climate-related variables temperature and discharge, we performed periodic and targeted (summer and winter) field sampling campaigns at the Thur River, Switzerland, and laboratory column experiments simulating the field conditions. Typical summer and winter field conditions could be successfully simulated by the column experiments. Dissolved organic matter (DOM) was found not to be a major electron donor for aerobic respiration in summer and the DOM consumption did not reveal a significant correlation with temperature and discharge. It is hypothesized that under summer conditions, organic matter associated with the aquifer material (particulate organic matter, POM) is responsible for most of the consumption of dissolved oxygen (DO), which was the most important electron acceptor in both the field and the column system. For typical summer conditions at temperatures >20 °C, complete depletion of DO was observed in the column system and in a piezometer located only a few metres from the river. Both in the field system and the column experiments, nitrate acted as a redox buffer preventing the release of manganese(II) and iron(II). For periodic field observations over five years, DO consumption showed a pronounced temperature dependence (correlation coefficient r = 0.74) and therefore a seasonal pattern, which seemed to be mostly explained by the temperature dependence of the calculated POM consumption (r = 0.7). The river discharge was found to be highly and positively correlated with DO consumption (r = 0.85), suggesting an enhanced POM input during flood events. This high correlation could only be observed for the low-temperature range (T < 15 °C). For temperatures >15 °C, DO consumption was already high (almost complete) and the impact of discharge could not be resolved. Based on our results, we estimate the risk for similar river-infiltration systems to release manganese(II) and iron(II) to be low during future average summer conditions. However, long-lasting heat waves might lead to a consumption of the nitrate buffer, inducing a mobilization of manganese and iron.     

Sources and transport of selected organic micropollutants in urban groundwater underlying the city of Halle (Saale), Germany

In this study, we used isotopic (delta18O, delta2H, delta34S-SO4) and chemical tracers (boron) to assess the sources and transport processes of the micropollutants carbamazepine, galaxolide, and bisphenol A in groundwater underlying the city of Halle (Saale), Germany. Their ubiquitous presence in urban groundwater results from a combination of local river water infiltration, sewer exfiltration, and urban stormwater recharge. Attenuation during transport with infiltrating river water increased from carbamazepine (0-60%) to galaxolide (60-80%) in accordance with their increasing sorption affinity and decreasing recalcitrance against biodegradation. Distinctly higher attenuation during transport was found for carbamazepine (85-100%) and galaxolide (95-100%) if micropollutants originated from sewer exfiltration. Most likely, this is related to higher contents of organic matter and higher transit times of the respective flow paths. Although attenuation undoubtedly also affects the transport of bisphenol A, quantification is limited due to additional contributions from the urban stormwater recharge. As a consequence, micropollutant loads in groundwater indicate that groundwater discharge may dominate the export of bisphenol A from urban areas.     

Molecular beam epitaxy grown GeSn p-i-n photodetectors integrated on Si

GeSn p-i-n photodetectors with a low Sn mole fraction made by molecular beam epitaxy on Si substrates show higher optical responsivities for wavelength λ > 1400 nm compared with p-i-n photodetectors made from pure Ge. The Sn incorporation in Ge is done by a low temperature growth step in order to minimize Sn segregation. The Sn incorporation and the alloy content are investigated by μ-Raman spectroscopy and calibrated Secondary Ion Mass Spectrometry. The photodetectors are manufactured with sharp doping transitions and are realized as double mesa structures with diameters from 1.5 μm up to 80 μm. The optical measurements are carried out with a broadband super continuum laser from λ = 1200 nm up to λ = 1700 nm. At a wavelength of λ = 1550 nm the optical responsivity of these vertical GeSn diodes is 0.1 A/W. In comparison with a pure Ge detector of the same geometrical dimensions the optical responsivity is increased by factor of three as a result of Sn caused band gap reduction.     

Grundwasser – Online-Submission

Ohne Zusammenfassung     

Wie Flussbett-Temperaturdaten zur Ermittlung der Aquifer-Heterogenität beitragen können

Groundwater discharge to a stream may show small-scale heterogeneities caused by the structure of the connected aquifer. The spatial pattern of the groundwater discharge can be investigated by temperature measurements in the streambed. Thus, the heterogeneity of the hydraulic conductivity (K) of an aquifer can be inferred from measured streambed temperatures. A flow and heat transport model of a stream-aquifer system was set up including stochastic K fields generated from the mean and variance of K data obtained from direct-push measurements. However, the simulated streambed temperatures did not cover the range of measured temperatures. Therefore, the relation between the distribution of streambed temperatures and the variance of K was used to calibrate the variance of K such that the distribution of measured temperatures could be reproduced in the model. This study showed that methods based on heat as a parameter for measuring and calibration constitute a valuable supplement to traditional investigation techniques.