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151.
DNA- and RNA-hydrolyzing antibodies from the blood of patients with various forms of viral hepatitis
AG Baranovsky VG Matushin AV Vlassov VG Zabara VA Naumov R Giege VN Buneva GA Nevinsky 《Canadian Metallurgical Quarterly》1997,62(12):1358-1366
Antibodies (Abs) hydrolyzing proteins, DNA, and RNA are detected in the blood of patients with various autoimmune diseases. In the present work, homogeneous preparations of IgG Abs from the blood of the healthy donors as well as patients with A, B, C, and delta types of viral hepatitis, influenza, pneumonia, tuberculosis, tonsillitis, duodenal ulcer, and some types of cancer were purified. For the first time, the fraction of IgG and its Fab fragments of patients with viral hepatitis were shown to have high DNA- and RNA-hydrolyzing activity. In case of Abs from the healthy donors and patients with other diseases, high activity of Abs was not detected. The data obtained by various methods indicate that the activity of hepatitis Abs is an intrinsic property of the immunoglobulins. The relative rates of hydrolysis of cCMP, poly(U), poly(A), poly(C), and tRNA(Phe) by hepatitis Abs were compared with those of RNase A and other RNases from human blood. Significant differences in activities of Abs and nucleases in hydrolysis of model substrates were demonstrated. Thus, catalytically active Abs can appear in the blood of patients not only with autoimmune disorders, but with viral diseases as well. 相似文献
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TJ Resink VN Bochkov AW Hahn MP Philippova FR Bühler VA Tkachuk 《Canadian Metallurgical Quarterly》1995,32(5):328-338
The mitogenic activities of low (LDL)- and high (HDL)-density lipoproteins have been examined in cultures of human vascular smooth muscle cells (VSMC). LDL and HDL3 dose-dependently (EC50 values approximately 50 micrograms/ml) stimulated DNA and protein synthesis ([3H]-thymidine and [3H]-leucine incorporation, respectively) in the absence of exogenously added mitogens. The synthetic responses of VSMC to combinations of LDL and HDL3 were additive, indicating that each lipoprotein mediates discrete effects. LDL or HDL3 promoted VSMC proliferation under strict mitogen-free conditions, but this growth response was not sustained. VSMC exposed to combinations of lipoproteins (either LDL or HDL3) and growth factors (either PDGF-BB, EGF, bFGF or IGF) exhibited synergistic DNA synthesis responses. In the combined presence of PDGF-BB and either LDL or HDL3, VSMC proliferation was sustained. Anionized lipoprotein preparations (oxidized, acetylated, carbamylated or malonimylated) also stimulated DNA and protein synthesis. Since the antioxidant beta-hydroxylated toluene did not block the effect of native LDL on DNA synthesis, and fucoidin, a specific competitor for the 'scavenger' receptor, did not inhibit oxidized LDL-induced DNA synthesis, activation of mitogenic signals by lipoproteins does not depend on lipid peroxidation. Rather, the apparent intrinsic mitogenic potential of lipoproteins may depend upon their direct activation of replication-coupled signal transduction systems. 相似文献
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VA Kholodny? IF Syrbu MM Militsa VP Kovalenko VO Zhulin'sky? OV Kapshytar 《Canadian Metallurgical Quarterly》1997,(11-12):10-11
While examination of 83 patients it was established that in the presence of complicated gastric and duodenal ulcer disease the secondary immune deficiency is developing, which deepens after the surgical intervention conduction. Tiotriazolin, promoting the immune system state and the blood biochemistry indexes normalization, the immune system state and the blood biochemistry indexes stimulation, hemopoiesis stimulation, the treatment results improvement, was applied for immunomodulation. 相似文献
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Restriction fragment length polymorphism (RFLP) of the apolipoprotein B locus was investigated in the population of Tomsk. The frequencies of alleles and haplotypes of polymorphic restriction sites XbaI, MspI, and EcoRI were determined. The parameters of linkage disequilibrium and polymorphism information content (PIC) were estimated. Comparative data on various regions of the world are shown. 相似文献
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