The interaction of human plasma low density lipoproteins with glycosamino-glycans: Influence of the chemical composition

Human plasma of 5 normolipemic individuals was incubated for 24 hr at 37 C in the presence or in the absence of lecithin: cholesterol acyltransferase (LCAT)-inhibitors. Plasma steored at 4 C served as a control. The low density lipoprotein (LDL) fractions of the samples were isolated and investigated with respected to changes in chemical composition and complexing activity with glycosamino glycans (GAG). Incubation of plasma in the presence of LCAT inhibitors caused a significant increase of LDL triglycerides at the expense of cholesteryl esters. Incubation with active LCAT not only changed the core but also the surface constituents (decrease in phospholipids and in free cholesterol). The amount of GAG bound per mg of LDL was not uniformly changed in samples incubated after LCAT inhibition. LDL isolated from plasma incubated in the presence of LCAT, on the other hand, showed a significant reduction in GAG binding. The ratio of free cholesterol: GAG in the complex was most significantly reduced in LCAT-modified LDL. There was in addition a highly significant correlation between the LDL:GAG ratio in the complex and the free cholesterol and phospholipid content of the LDL samples. It is concluded that alterations in surface lipid constitutents of LDL strongly after their interaction with sulfated polysaccharides, an effect which may be relevant also in vivo for the interaction of LDL with cell surfaces and intercellular matrices.     

Radioaktive Indikatormethode in der chemischen Verfahrenstechnik

The radioactive tracer method in the chemical industry. In the chemical industry the radioactive tracer method has become a method of analysis with a variety of applications, which range from determining traces of biologically active substances to process analysis in production plants. Process analysis, for instance the determination of residence-time distributions or of intermingling of streams of different substances, can be carried out under widely varying operating conditions. If the streams of material are labelled with gamma-ray emitters the distribution of the tracers can be determined by means of detectors outside the plant. To demonstrate the possible applications of the method to process analysis, measurements in three different plants are described. Exhaust gases were labelled with Ar-41 to determine the residence-time distribution in plant for the combustion of waste residues consisting of a rotary tube furnace, an afterburn chamber, and a waste-heat boiler. The waste water entering the activation tank of sewage-treatment plant was labelled with Na-24 to determine its residence-time distribution. Individual particles labelled with Mn-56 were used to investigate mixing of the powder in the stirred-bed reactor of a polypropylene plant. The knowledge gained from these measurements has contributed to optimization of existing and planned plant.     

Alina Szeminska.

Draws attention to the omission of Dr. Alina Szeminska's name from the English translation of The Child's Conception of Number. The book, published in French in 1941, was coauthored by Jean Piaget and Szeminska. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Rate of tetracycline dissolution from tablets and capsules and the biological availability of the antibiotic

Relation between the rate of tetracycline dissolution from tablets and capsules and the biological acceptibility of the antibiotic in the host was studied. The antibiotic dissolution rate from the pharmaceutical forms was determined in a modernized apparatus "Rotary basket" in water, the speed of the basket rotation was 200 r.p.m. In addition the tetracycline blood levels in patients treated with the drug in the above pharmaceutical forms were estimated. It was found that the rate of the antibiotic dissolution characterized the antibiotic biological acceptibility. A test for the dissolution rate was developed. It may be used for estimation of production batches of tetracycline tablets.     

Hardness results for approximate pure Horn CNF formulae minimization

We study the hardness of approximation of clause minimum and literal minimum representations of pure Horn functions in n Boolean variables. We show that unless P=NP, it is not possible to approximate in polynomial time the minimum number of clauses and the minimum number of literals of pure Horn CNF representations to within a factor of \(2^{\log^{1-o(1)} n}\) . This is the case even when the inputs are restricted to pure Horn 3-CNFs with O(n ^1+ε ) clauses, for some small positive constant ε. Furthermore, we show that even allowing sub-exponential time computation, it is still not possible to obtain constant factor approximations for such problems unless the Exponential Time Hypothesis turns out to be false.     

Visualizing Oxidative Cellular Stress Induced by Nanoparticles in the Subcytotoxic Range Using Fluorescence Lifetime Imaging

Nanoparticles hold a great promise in biomedical science. However, due to their unique physical and chemical properties they can lead to overproduction of intracellular reactive oxygen species (ROS). As an important mechanism of nanotoxicity, there is a great need for sensitive and high‐throughput adaptable single‐cell ROS detection methods. Here, fluorescence lifetime imaging microscopy (FLIM) is employed for single‐cell ROS detection (FLIM‐ROX) providing increased sensitivity and enabling high‐throughput analysis in fixed and live cells. FLIM‐ROX owes its sensitivity to the discrimination of autofluorescence from the unique fluorescence lifetime of the ROS reporter dye. The effect of subcytotoxic amounts of cationic gold nanoparticles in J774A.1 cells and primary human macrophages on ROS generation is investigated. FLIM‐ROX measures very low ROS levels upon gold nanoparticle exposure, which is undetectable by the conventional method. It is demonstrated that cellular morphology changes, elevated senescence, and DNA damage link the resulting low‐level oxidative stress to cellular adverse effects and thus nanotoxicity. Multiphoton FLIM‐ROX enables the quantification of spatial ROS distribution in vivo, which is shown for skin tissue as a target for nanoparticle exposure. Thus, this innovative method allows identifying of low‐level ROS in vitro and in vivo and, subsequently, promotes understanding of ROS‐associated nanotoxicity.     

Self‐adaptation of service level in distributed systems

Modern distributed systems are diverse and dynamic, and consequently difficult to manage using traditional approaches, which rely on an extensive initial knowledge of the system. On the performance front, these systems often offer multiple opportunities for dynamically degrading or improving service level based on workload intensity, to avoid overload and underload. In this context, we propose a novel approach for building distributed systems capable of autonomously deciding when and how to adapt service level. Our approach limits the knowledge that must be provided manually to a component‐based representation of the system. From this representation, we build and maintain a performance profile, which allows us to (1) identify the most promising adaptations based on workload type and (2) dynamically characterize the intrinsic efficiency of each adaptation based on past attempts. We have successfully implemented and evaluated a prototype of our approach in the context of multi‐tiered application servers. Copyright © 2010 John Wiley & Sons, Ltd.     

A?novel method for the quantitative analysis of the water content in lignocellulosic material

A novel method for the determination of the water content in wood has been developed and experimentally tested. Based on the reaction of trimethylchlorosilane with various wood constituents and water, the generation of a by-product (hexamethyldisiloxane) was pursued and quantified via gas-chromatography and the water content was subsequently calculated back. The experiments were carried out with preconditioned spruce wood meal (particle sizes ranging between 60 and 140 microns) using water free solvents and reagents in a water free reaction atmosphere. The results showed that the investigated samples incorporated around 10 wt. % of water after preconditioning for 48 h.