The beta-adrenomimetic effect of human and animal blood serum

The conventional analysis and design of highway bridges ignore the contribution of sidewalks and∕or railings in a bridge deck when calculating the flexural strength of superstructures. The presence of sidewalks and railings or parapets acting integrally with the bridge deck have the effect of stiffening the outside girders and attracting more load while reducing the load effects in the interior girders. This paper presents the results of a parametric study showing the influence of typical sidewalks and railings on wheel load distribution as well as on the load-carrying capacity of highway bridges. A typical one-span, two-lane, simply supported, composite steel girder bridge was selected in order to investigate the influence of various parameters such as: span length, girder spacing, sidewalks, and railings. A total of 120 bridges were analyzed using three-dimensional finite-element analysis. American Association of State Highway and Transportation Officials (AASHTO) HS20 design trucks were positioned in both lanes to produce the maximum moments. The finite-element analysis results were also compared with AASHTO wheel load distribution factors. The AASHTO load and resistance factor design (LRFD) wheel load distribution formula correlated conservatively with the finite-element results and all were less than the typical empirical formula (S∕5.5). The presence of sidewalks and railings were shown to increase the load-carrying capacity by as much as 30% if they were included in the strength evaluation of highway bridges.     

Estimate of stiffness of the cellulose cuprammonium complex macromolecule in solution

Conclusions A tentative estimate of the stiffness (from the Kuhn segment) of the cuprammonium complex of cellulose (CCC) in solution has been given; it is about 200 A.The calculated concentration for transition of a CCC solution into the liquid-crystal state is in excess of 27% by wt.Translated from Khimicheskie Volokna, No. 3, pp. 23–24, May–June, 1988.     

Saccharide-assisted delivery of cytotoxic liposomes to human malignant cells

The overexpression of lectins by malignant cells was applied for in vitro targeting of liposomes equipped with a saccharide vector and loaded in the lipid phase with a lipid derivative of anticancer agent sarcolysine. The lectin specificity of human leukemia HL-60 and human lung adenocarcinoma ACL cells was revealed by tests with fluorescein-labeled sugar probes. With the help of fluorescent lipid dye it was shown that active saccharide ligands increased the level of the vectored liposome binding to malignant cells by 50-80% as compared to liposomes without vector or with inactive one. The degree of liposome/cell membrane fusion was monitored fluorometrically and was shown to be complete and independent of the vectors. The targeted drug-loaded liposomes had the cytotoxic activity 2-4 times higher as compared to the vector-free ones.     

The deamination of L-arginine and the synthesis of active nitrogen compounds in Yersinia pestis

The investigations of authors concerning infants and their families were generalized on the basis of the observations of infants and their parents with some psychological problems in families. The principles of psychotherapeutic work in above-mentioned groups were formulated. Besides the methods of the diagnosis of psychical deviations development in first year infants as well as peculiarities of alterations in mother-baby system and the main forms of psychic disturbances correction in infants were also described.     

Dynamics of clot growth induced by thrombin diffusing into nonstirred citrate human plasma

We have tested whether action potential-evoked Ca2+ influx is required to initiate clathrin-mediated synaptic vesicle endocytosis in the lamprey reticulospinal synapse. Exo- and endocytosis were temporally separated by a procedure involving tonic action potential stimulation and subsequent removal of extracellular Ca2+ (Ca2+e). A low concentration of Ca2+ ([Ca2+]e of 11 microM) was found to be required for the induction of early stages of endocytosis. However, the entire endocytic process, from the formation of clathrin-coated membrane invaginations to the generation of synaptic vesicles, proceeded in the absence of action potential-mediated Ca2+ entry. Our results indicate that the membrane of synaptic vesicles newly incorporated in the plasma membrane is a sufficient trigger of clathrin-mediated synaptic vesicle endocytosis.     

Functional morphology of nucleolus organizer regions of chromosomes and nucleoli in human multiple myeloma cell lines. I. Variation of the morphology and silver staining of nucleolus organizer regions of chromosomes in RMPI 8226 and U 266 cell lines with different level of differentiation of during 7 days after cell passage

The morphology and Ag-staining of nucleoli in human multiple myeloma cell lines RPMI 8226 and U 266, distinguished from each other in the differentiation degree, were quantitatively studied, and the production of immunoglobulins or their fragments by the line cells was evaluated throughout 7 days after cell seeding. The less differentiated cell line RPMI 8226 and the high differentiated cell line U 266 were revealed to differ in both the initial level of immunoglobulin production and dynamics of immunoglobulin accumulation in culture medium. The total number of Ag-stained nucleolar-organizer regions (AgNORs) per nucleus in cells RPMI 8226 was significantly higher than in cells U 266 in all times after seeding of the cells. In both cell lines changes in the quantity and shape of nucleoli and also in the total number of AgNORs per nucleus and pattern of AgNORs distribution within nucleoli correlated with the cell cycle phase. Relationships between morphofunctional changes in nucleoli and the differentiation degree and proliferative activity of the cells, and also between the number of Ag-positive nucleolar-organizing metaphase chromosomes and the functional activity of interphase AgNORs are discussed.