Age-related dynamics of the genetic marker distribution in an irradiated population. Haptoglobin genetic system

The age dynamics of haptoglobin (Hp) type and allele distribution was studied in 985 workers aged 35-79 years who worked in the atomic industry. These workers were exposed to chronic radiation in a wide range of doses 17-40 years ago. Along with the chi 2 test, the relative and attributive risks of elimination of Hp types and alleles were determined to obtain the qualitative characteristics of age alterations. The substantial changes in distribution of Hp types and alleles were revealed in individuals older than 60 years. These changes depended on doses of external and internal irradiation. For instance, in individuals of this age group, who received the total dose of gamma-radiation (less than 100 cGy) and or that of plutonium-239 incorporation (less than 1.48 kBq), a decrease in Hp 2-2 and Hp2 and an increase in Hp 1-1 and Hp1 was observed. However, among the individuals of similar age (older than 62 years), who received a higher total dose of external and/or internal radiation, the different Hp type distribution showed a decrease in Hp 2-1 and an increase in Hp 1-1. In the latter case, the changes with age were dose-dependent. In individuals over 60 years of age with essential hypertension or diabetes mellitus and those who had a myocardial infarction, a decrease in Hp 2-2 and Hp2 and an increase in Hp 1-1 and Hp1 were found. This explains the changes with age in individuals irradiated at lower doses. The peculiar changes with age in individuals irradiated at higher doses are possibly due to the difference in morbidity and mortality from malignancy in various Hp type carriers.     

Dormant forms of Yersinia pseudotuberculosis during interaction with green algae and their exometabolites (population dynamics and ultrastructure)

The dynamics of the transition of Y.pseudotuberculosis to the latent (noncultivable) state in sterile soil extraction and in soil extraction, containing Scenedesmus algae or their exometabolites, was evaluated by the bacteriological method and with the use of polymerase chain reaction. The formation of Y.pseudotuberculosis latent forms achieved its highest rate under the action of algal exometabolites (on day 11), while in the presence of algal cells such formation was delayed to 3-5 months. The partial reversion of Y.pseudotuberculosis nonculativable forms to vegetative, bacteriologically detectable forms occurred under the action of fetal serum, as well as live or killed infusoria. Transmission electron microscopy demonstrated that noncultivable Y.pseudotuberculosis had a round form, diminished cell size, denser cytoplasm and enlarged cytoplasmic space. The reversible transition of bacteria to the latent state is regarded as their adaptation to unfavorable factors during their existence in soils and water reservoirs.     

Choice of the optimum intersection point for beam radiation axis in the planning of radiotherapy

Destructive changes in cardiomyocyte mitochondria of patients with diagnosis of alcoholic heart lesion were observed by the ultrastructural analysis of the endocardial biopsies serial sections. The appearance of mitochondria with the third additional compartment and mitochondria having two zones of different electron density not separated into the special compartments are described for the first time. The development of megamitochondria having different morphology is demonstrated. Mitochondrial origin of lipophuscin granules is suggested on the basis of the ultrastructural changes. Unusual structure of atypical mitochondria may provide new properties of these organelles this being considered by the authors as an adaptive cell and mitochondria response to the new metabolic conditions.     

The effect of catalase inhibitor 3-amino-1,2,4-triazole on coupled oxidative phosphorylation and the state of adenyl system in liver mitochondria of albino rats

Blepharoptosis is a recognized complication of cataract extraction and other ocular procedures. A 6-month waiting period is recommended before repairing postcataract ptosis because most cases resolve during this time. We report the course of a patient who developed spontaneous recovery of eyelid function 11 months after cataract surgery. She had previously undergone successful ptosis repair 8 months after cataract extraction, but developed overcorrection 3 months later as her levator function returned to normal. We recommend at least a 1-year waiting period before repair of postcataract ptosis. If corrective surgery is undertaken earlier than 1 year due to visual obstruction or for aesthetic reasons, we suggest two guidelines. The levator should be plicated rather than resected and patients should be forewarned of the possibility for a revisionary procedure if spontaneous return of levator function occurs.     

The genetic sequelae for plant populations of radioactive environmental pollution in connection with the Chernobyl accident

Populations of Arabidopsis thaliana (L.) Heynh., and Pinus sylvestris L., growing within 30 km of Chernobyl and Bryansk region have been analyzed for the frequency of embryonic lethal mutations on Arabidopsis and frequency of chlorophyll mutations and chromosome aberrations by pine. On pine also have been analyzed rate of mutations at enzyme loci in endosperms of seeds. Dose dependence of the value genetic damage on level of radioactive pollution was observed.