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21.
Although reticulocyte counts can be reliably performed for up to 48 h after storage in EDTA, it is unclear whether this is applicable to the pediatric age group. In order to evaluate this, manual reticulocyte counts were performed on 20 specimens from pediatric patients stored at 4 degrees C for up to 24 h post collection. Samples were evaluated at 1-3, 6, 12, 18, and 24 h after storage in EDTA vacutainer tubes at 4 degrees C. The age of the subjects ranged from 1 day to 9 years with a median age of 3 years. Patients' reticulocyte counts ranged from 0 to 27% (5.89 +/- 7.21). No clinically significant changes were evident in the reticulocyte count over 24 h after specimen collection. The mean of the 20 specimens at 1-3 h was 5.50 and at 24 h was 5.40 (P > .05). The standard deviation of the mean values ranged from 7.03 to 7.26 (P > .05). The results indicate that reticulocyte counts may be performed on previously drawn blood held at 4 degrees C for up to 24 h post collection in a pediatric population without significant difference from baseline values. 相似文献
22.
Avian cyclin D2 (Cyl D2)-encoding cDNA clones were isolated from a chicken UG9 T-cell lambda gt10 library. Sequence analysis revealed a high degree of sequence conservation with both the mouse and human Cyl D2, and somewhat lower similarity with the mouse and human Cyl D1 and D3. The homology is highest between species in the Cyl-box domain which is well conserved among human, mouse and chicken. A single 6.0-kb CYL2 mRNA is produced in both avian B- and T-cells, as expected. 相似文献
23.
Rachinger's method of separating 1 and 2 doublets in X-ray emission spectra is applied for the first time to separate the superimposed doublets due to spin-orbit coupling. This method has been applied to separate closely lying doublets such as (Ag 4p3/2and Ag 4p1/2, (Ni 3p3/2and Ni 3p1/2) and (Cu 3p3/2and Cu 3p1/2). The intensities ratios of the separated peaks are measured and compared with the ratios obtained from a first-order calculation. An excellent agreement between the measured and calculated ratios are obtained. Residual background intensities compared with the estimated values are found to agree within certain uncertainties. 相似文献
24.
A series of experiments were carried out on three commercial steels to explore the possibility for characterizing tempered martensite embrittlement (TME) by Rockwell, macro-, and micro-Vickers hardness tests. The results indicate distinct hardness peaks in two steels and an inflection in the other around the TME temperature. A new analytical approach for examining the slope of hardness-tempering temperature plots appears to reveal the TME phenomenon more sensitively. Dilatometric examinations substantiate that the temperature of hardness peak/inflection occurs beyond the second stage of tempering. 相似文献
25.
The concept of control signals is fundamental to digital design. It evokes the notion of one or more signals that influence computation on other signals in a system, and regulate the behavior of the system. A formal definition of control signals is presented. It is argued that knowledge of the control signals in a complex design such as a microprocessor or a computer is crucial to the comprehension and specification of the design and that knowledge of the control signals of a design leads to a more efficient hardware implementation. The identification of control signals in two microprocessors are presented as examples 相似文献
26.
Rates of coke formation during steam pyrolysis of naphtha have been investigated in a jet-stirred reactor both for sodium silicate coated and uncoated Inconel 600 surfaces in the temperature range of 1078–1108 K. Coke formation rates were significantly reduced on sodium silicate coated plates due to the passivation of the metal surface. However, the coking rates gradually increased with successive decokings of the coated surface. 相似文献
27.
The superplastic properties of a rapidly solidified, high strength P/M Al alloy and the same alloy reinforced with SiC particulates
(SiC
p
) have been studied. To prepare superplastic test materials, a matrix alloy powder of composition 7.2Zn-2.4Mg-2Cu-0.2Zr-0.12Cr-0.2Co
(Kaiser PM-64) and the powder mixed with 10 to 20 vol pct SiC
p
(~5 μm diameter) were thermomechanically processed to very fine equiaxed grain structures of ~6 μm and ~8 μm, respectively.
Superplasticity in these materials was evaluated by characterizing (1) high temperature stability, (2) dynamic grain growth,
(3) strain rate sensitivity, (4) flow stress behavior, (5) cavitation and cavitation control, and (6) total superplastic strain.
It was observed that the PM-64 alloy could achieve a total elongation of over 800 pct, while the SiCp reinforced alloy could attain an elongation greater than 500 pct before failure. Also, it was shown that with the use of
hydrostatic pressure during superplastic flow, cavitation could be controlled. Observations were made of the effect SiC
p
reinforcement particles had on the superplastic flow stress behavior. Interpretations are proposed to explain the role of
particulates during superplastic straining. 相似文献
28.
G. G. Krishna Murthy A. Ghosh S. P. Mehrotra 《Metallurgical and Materials Transactions B》1989,20(1):53-59
A macroscopic, steady state energy balance model has been formulated to describe mixing phenom-ena in a liquid bath stirred
by injecting gas through a straight nozzle fitted axially at the bottom of the vessel. This, along with experimental data
on a water model previously reported, was employed to make predictions. Input energy terms considered in the model consist
of buoyancy energy and empirically determined fraction of gas kinetic energy. Dissipation of energy was attributed to liquid
circulation and bubble slip. The two-phase plume was assumed to be a truncated cone whose dimen-sions depended upon operating
conditions. Numerical solution of model equations gave liquid velocity and gas hold-up inside the plume as well as liquid
circulation rate and liquid velocity in the region outside the plume. Influence of process variables, e.g., gas flow rate,
bath height, and nozzle diameter, have been predicted. Validity of the model has been established by comparing some pre-dicted
entrainment ratios with those experimentally measured by other investigators. Empirical cor-relations to predict circulation
time and circulation number have been proposed. Circulation number was found to vary between 2 and 12 in contrast to the existing
assumption in the literature of a con-stant value of 3. Usefulness of these correlations in predicting mixing time for industrial
vessels has been demonstrated.
Formerly a Graduate Student in the De-partment of Metallurgical Engineering at the Indian Institute of Technol-ogy, Kanpur 相似文献
29.
30.
DJ Benos BH Hahn JK Bubien SK Ghosh NA Mashburn MA Chaikin GM Shaw EN Benveniste 《Canadian Metallurgical Quarterly》1994,91(2):494-498
Infection by human immunodeficiency virus type 1 (HIV-1) is often complicated by a variety of neurological abnormalities. The most common clinical syndrome, termed acquired immunodeficiency syndrome (AIDS) dementia complex, presents as a subcortical dementia with cognitive, motor, and behavioral disturbances and is unique to HIV-1 infection. The pathogenesis of this syndrome is poorly understood but is believed to involve interactions among virally infected macrophages/microglia, astrocytes, and neurons. In this study, we show that exposure of primary rat and human astrocytes to heat-activated HIV-1 virions, or to eukaryotically expressed HIV-1 and HIV-2 envelope glycoproteins (gp120) stimulates amiloride-sensitive Na+/H+ antiport, potassium conductance, and glutamate efflux. These effects are blocked specifically by amiloride, an inhibitor of Na+/H+ antiport and by the selective removal of gp120 with immobilized monoclonal antibody. As a result of modulation of astrocytic function by gp120, the ensuing neuronal depolarization and glutamate exposure could activate both voltage-gated and N-methyl-D-aspartate-regulated Ca2+ channels, leading to increases in intraneuronal Ca2+ and neuronal death. These findings implicate the astrocyte directly in the pathogenesis of AIDS dementia complex. 相似文献