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101.
AA Zargarov II Senin AM Alekseev SV Shul''ga-Morsko? PP Filippov VM Lipkin 《Canadian Metallurgical Quarterly》1996,22(7):483-488
Numerous data strongly suggest the involvement of cytokines and the matrix metalloproteinase collagenase (MMP-1) in the pathogenesis of periodontitis. Recently, we have demonstrated that, upon culturing under the influence of IL-1 alpha + EGF, a large amount of inactive procollagenase (MMP-1) is stored in the extracellular matrix of periosteal tissue. We now show that this endogenous reservoir of proenzyme can be operative after activation with plasmin and is able to induce a rapid and almost complete breakdown of the collagenous extracellular matrix. The level of collagen degradation following activation showed a strong correlation with the amount of proenzyme that was incorporated in the tissue. The highest level of degradation (70% of the total amount of collagenous proteins) was found with the IL-1 alpha + EGF-treated explants, followed by those treated with IL-1 alpha alone (35%). Explants cultured with EGF or in the absence of cytokines, containing only small amounts of procollagenase, showed little collagen breakdown following plasmin activation (7%). Inhibition of metalloproteinases by EDTA, or blockage of plasmin by PMSF, prevented the degradation in all explants irrespective of the amount of proenzyme present in the tissue. Our findings demonstrate that endogenous proenzyme stored in a native connective tissue matrix can be activated at a later time interval which results in a massive breakdown of the tissue. This study shows a possible pathway of collagenase-induced breakdown without recent de novo synthesis of the enzyme. Such a sequence may be operative in chronic inflammatory diseases, such as periodontitis, where production of procollagenase under the influence of cytokines spans a longer time period, whereas breakdown is often characterized by a cyclic behaviour. 相似文献
102.
H Ramirez-Gil E Feldberg VM Almeida-Val AL Val 《Canadian Metallurgical Quarterly》1998,31(11):1449-1458
Karyological characteristics, i.e., diploid number, chromosome morphology and nucleolus organizer regions (NORs), biochemical characteristics, i.e., electrophoretic analysis of blood hemoglobin and the tissue enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), and phosphoglucose isomerase (PGI), and physiological characteristics, i.e., relative concentration of hemoglobin and intraerythrocytic concentrations of organic phosphates were analyzed for the species Callophysus macropterus collected from Marchantaria Island (white water system--Solim?es River) and Anavilhanas Archipelago (black water system--Negro River). Karyological and biochemical data did not reveal significant differences between specimens collected at the two sites. However, the relative distribution of hemoglobin bands I and III (I = 16.33 +/- 1.05 and III = 37.20 +/- 1.32 for Marchantaria specimens and I = 6.33 +/- 1.32 and III = 48.05 +/- 1.55 for Anavilhanas specimens) and levels of intraerythrocytic GTP (1.32 +/- 0.16 and 2.76 +/- 0.18 for Marchantaria and Anavilhanas specimens, respectively), but not ATP or total phosphate, were significantly different, indicating a physiological adaptation to the environmental conditions of these habitats. It is suggested that C. macropterus specimens from the two collecting sites belong to a single population, and that they adjusted some physiological characteristics to adapt to local environmental conditions. 相似文献
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AI Necha? VM Sitenko KV Novikov VV Stukalov VT Drobot 《Canadian Metallurgical Quarterly》1980,125(10):27-35
Concrements were found in the ducts of 172 patients subjected to secondary surgical interventions on bile ducts. The detailed analysis of the data failed to answer the question whether the stones could be formed immediately in the ducts without such conditions as a mechanical obstacle for the outflow of bile, the presence of foreign bodies and a big stump of the cystic duct. Such a formation of stones can take place in very few cases. The authors have shown that control cholangiography and especially choledochoscopy can reduce the danger to leave the stones in the ducts, but does not eliminate the danger completely. Nevertheless they think that now it is possible to lessen the indications for biliodigestive anastomoses in choledocholithiasis since the retained stones can be revealed within the first days after surgery and, as a rule, removed with instruments or by other methods using the drainage left in the ducts. 相似文献
109.
We consider the problem of resequencing a set of prearranged jobs when there is limited resequencing flexibility and sequence-dependent changeover costs. Resequencing flexibility is limited by how far forward or backward a job can shift in the sequence relative to its original position. We show how the problem can be solved using dynamic programming in polynomial time with respect to the number of jobs. We also show how the same solution approach can be extended to problems where sequencing constraints are job specific and to problems where job features, which determine changeover costs, are jointly determined with the job sequence. We provide an integer programming formulation to the resequencing problem whose linear programming relaxation offers a useful lower bound. We also describe a family of decomposition heuristics that are easy to customize to provide desired levels of solution quality and solution time. We document the quality of the lower bound from the linear programming relaxation and the upper bound from the heuristic using numerical results. We also provide numerical results to support managerial insights regarding the value of flexibility. We show that the value of flexibility is of the diminishing kind with most of the benefit realized with relatively limited flexibility. We also show that a balanced allocation of flexibility among forward and backward position shifting is superior to an unbalanced one. More significantly, we show that forward and backward position shifting flexibility are complements with the value of one increasing in the amount of the other. Finally, we apply our solution approach to a real-world case from the automotive industry. 相似文献
110.
This paper emphasizes total self-development of the individual for improved motivation and organization management. It builds on Maslow's hierarchy of needs theory to examine motivational levels for four levels of engineering staff at a public construction agency. The researchers studied these engineering groups qualitatively through interviews and quantitatively using a questionnaire. Using a holistic approach, this study focused on 15 parameters from Maslow's five essential needs—the physical, safety, social, esteem, and self-actualization levels. Considerable emphasis was placed on the development of Maslow's principle of “self-actualization.” This difficult-to-grasp concept, as Maslow reported, is a prerequisite for enlightened management. The researchers analyzed engineers' perceptions regarding the fulfillment of need parameters and measured their perception of the importance of those parameters. Among specific findings were that junior project engineers had higher scores on self-actualization than senior engineers. Findings showed that changes are desirable for satisfying meaningfulness of tasks, increasing self-sufficiency in doing the job, and improving individuality and sense of mission of employees. The application of the findings based on Maslow's model directly benefits the agency studied and other organizations in development programs. It also helps in increasing morale. 相似文献