The spectrum of respiratory disease associated with exposure to metal working fluids

Occupational respiratory diseases have been reported following exposure to metal working fluids. We report a spectrum of respiratory illnesses occurring in an outbreak in 30 workers of an automobile parts engine manufacturing plant. Workers presented with respiratory complaints and, after clinical and laboratory evaluations, were classified as those having hypersensitivity pneumonitis, occupational asthma, or industrial bronchitis, or those without occupational lung disease. Hypersensitivity pneumonitis affected seven workers, with six exhibiting serum precipitins to Acinetobacter Iwoffii. Occupational asthma and industrial bronchitis affected 12 and six workers, respectively. Oil-mist exposures were below current recommendations. Gram-negative bacteria, but no fungi, Thermophiles, or Legionella, were identified. Although specific agents responsible for each individual case could not be identified, probably both specific sensitizing agents and non-specific irritants from metal working fluids, additives, or contaminants contributed to this spectrum of occupational respiratory illness.     

The immune response of cattle, persistently infected with noncytopathic BVDV, after superinfection with antigenically semi-homologous cytopathic BVDV

The present study was undertaken to investigate firing patterns, locations, and projections to the phrenic motor nucleus of respiratory neurons in medullary raphe nuclei of rat. Experiments were performed on spontaneously breathing rats anesthetized with sodium pentobarbital. Extracellular spikes of single respiratory neurons were explored in midline medullary tegmentum. A total of 107 respiratory neurons was recorded in the raphe magnus, obscurus and pallidus. They were classified into the following eight types based on the relation of their firing patterns to the phase of respiration: (1) Inspiratory (I) throughout (n = 42); (2) I-late (n = 9); (3) I-decrementing (n = 1); (4) Pre-I (n = 2); (5) I-frequency modulated (n = 13); (6) Post-I (n = 12); (7) Expiratory (E) (n = 23) and (8) E-frequency modulated neurons (n = 5). Twenty of the 45 respiratory neurons examined were antidromically activated from the phrenic motor nucleus at the C4 spinal level with thresholds of 2-58 microA and latencies of 0.4-2.4 ms. Among the 20 neurons, 11 neurons were I-throughout, five were I-frequency modulated and four were E neurons. These results suggest that there is a population of neurons in the medullary raphe nuclei that projects to the phrenic motor nucleus at the C4 spinal level. It is possible that this projection may, in part, mediate the control of the diaphragmatic muscle motor neurons located in the C4 segments.     

Multivariate analysis of demographic parameters of the population

Total selection indices and their components in the Kursk Oblast, calculated from Crow's equations, are: Itot = = 0.403, I(f) = 0.345, Im = 0.043. The selection intensity was higher in rural than in urban populations. The levels of total selection and differential fertility in rural populations depended on the genetic and demographic structure of these populations. An increase of homozygosity in the rural populations (decreases in the mean square distance between spouses' birthplaces and in the frequency of long-range migrations, increase in ethnic assortativeness, etc.) increased the selection intensity.     

Surgical treatment of lymphadenopathy in children

Clinical observations of 1365 children with the lymph nodes affection were summarized. The diagnosis verification was conducted using the broad arsenal of general clinical and special methods of investigation. In complicated situations the histochemical and immunogenetic methods were applied for the diagnosis. The structure and dynamics of children's morbidity with lymphadenopathies (LAP) was studied up, the age, sexual and seasonal peculiarities were systematized, the analysis of primary localizations and clinico-morphological comparisons was conducted. The basic thesises of the standardized approach were elaborated on the ground of the diagnostical and tactical mistakes results analysis.     

Thermal denaturation of ribonuclease A characterized by water 17O and 2H magnetic relaxation dispersion

Water oxygen-17 and deuteron nuclear magnetic relaxation dispersion (NMRD) measurements were used to characterize ribonuclease A (RNase A) in the course of thermal denaturation at pH 2 and 4. The structure and dynamics of the protein were probed by specific long-lived water molecules, by the short-lived surface hydration, and by labile side-chain hydrogens. The NMRD data show that native RNase A contains at least three water molecules with a mean residence time of 8 ns at 27 degreesC and an activation enthalpy of ca. 40 kJ mol-1. These water molecules are identified with some or all of six ordered water molecules partly buried in surface pockets in the crystal structure of RNase A. The loss of the 17O dispersion at higher temperatures demonstrates that, in the thermally denatured protein, these surface pockets are either not present or undergoing large structural fluctuations on a subnanosecond time scale. The relaxation dispersion step vanishes monotonically and essentially in concert with the CD denaturation curves, thus ruling out the existence of equilibrium intermediates with a substantial amount of non-native and long-lived hydration water. The NMRD data show that thermally denatured RNase A has a relatively compact but highly flexible structure. The global solvent exposure and the hydrodynamic volume of the denatured protein are much less than for maximally unfolded disulfide-intact RNase A. The NMRD data show that thermal denaturation is accompanied by a large reduction of the mean-square orientational order parameter of side-chain O-H bonds, implying that, in the denatured state, these side chains sample a wide distribution of conformational states on a subnanosecond time scale.     

Decompression sickness-one of the vital problems of aerospace medicine

The author reviews the literature on decompression sickness (DCS) constituting one of the major problems of aerospace medicine. He speculates on the terms describing this health condition and offers the retrospective of hypothesised causes for DCS development. The paper outlines main DCS symptoms and reports statistics on the DCS incidence rate in flying personnel when piloting aircraft and training in altitude chambers, and in volunteered test-subjects during physiological experiments with simulated ascents in order to mimic the extravehicular activities of cosmonauts and to test the altitude gear. Underlined is the value of publications by many Russian and foreign investigators who contributed significantly to development of the scientific and applied aspects of this problem. The currently available and theoretically possible countermeasures against DCS in cosmonauts during EVA are considered.     

Neuronal responses in visual areas MT and MST during smooth pursuit target selection

We recorded the activity of single neurons in the middle temporal (MT) and middle superior temporal (MST) visual areas in two macaque monkeys while the animals performed a smooth pursuit target selection task. The monkeys were presented with two moving stimuli of different colors and were trained to initiate smooth pursuit to the stimulus that matched the color of a previously given cue. We designed these experiments so that we could separate the component of the neuronal response that was driven by the visual stimulus from an extraretinal component that predicted the color or direction of the selected target. We found that for all cells in MT and MST the response was primarily determined by the visual stimulus. However, 14% (8 of 58) of MT neurons and 26% (22 of 84) of MST neurons had a small predictive component that was significant at the P < or = 0.05 level. In some cells, the predictive component was clearly related to the color of the intended target, but more often it was correlated with the direction of the target. We have previously documented a systematic shift in the latency of smooth pursuit that depends on the relative direction of motion of the two stimuli. We found that neither the latency nor the amplitude of neuronal responses in MT or MST was correlated with behavioral latency. These results are consistent with a model for target selection in which a weak selection bias for the intended target is amplified by a competitive network that suppresses motion signals related to the nonintended stimulus. It is possible that the predictive component of neuronal responses in MT and MST contributes to the selection bias. However, the strength of the selection bias in MT and MST is not sufficient to account for the high degree of selectivity shown by pursuit behavior.     

Activation of phospholipase C gamma by PI 3-kinase-induced PH domain-mediated membrane targeting

Signaling via growth factor receptors frequently results in the concomitant activation of phospholipase C gamma (PLC gamma) and phosphatidylinositol (PI) 3-kinase. While it is well established that tyrosine phosphorylation of PLC gamma is necessary for its activation, we show here that PLC gamma is regulated additionally by the lipid products of PI 3-kinase. We demonstrate that the pleckstrin homology (PH) domain of PLC gamma binds to phosphatidylinositol 3,4,5-trisphosphate [PdtIns(3,4,5)P3], and is targeted to the membrane in response to growth factor stimulation, while a mutated version of this PH domain that does not bind PdtIns(3,4,5)P3 is not membrane targeted. Consistent with these observations, activation of PI 3-kinase causes PLC gamma PH domain-mediated membrane targeting and PLC gamma activation. By contrast, either the inhibition of PI 3-kinase by overexpression of a dominant-negative mutant or the prevention of PLC gamma membrane targeting by overexpression of the PLC gamma PH domain prevents growth factor-induced PLC gamma activation. These experiments reveal a novel mechanism for cross-talk and mutual regulation of activity between two enzymes that participate in the control of phosphoinositide metabolism.     

Volume of reaction by the Archibald ultracentrifuge method (lobster hemocyanin)

Samples of lobster hemocyanin (Homarus americanus) under conditions of reversible reaction between whole (25 S) and half (17 S) molecules have been subjected to accurately known nitrogen pressures in analytical ultracentrifuge cells. A modified pressurization chamber of the type developed by Schumaker and colleagues has been constructed for this purpose. The molecular weight was then determined at the top (liquid-gas) meniscus, by means of the Archibald method. The logarithmic dependence upon pressure of the derived equilibrium constant then gave directly the volume of reaction. Experiments were performed in veronal-citrate buffers at pH 8, where the molar volume of formation of whole (dodecameric) molecules from half molecules appears to be negative, and at pH 8.46 in veronal-citrate buffer in the presence of 0.003 molar free calcium ion, where the molar volume of formation was estimated to be + 390 cm3/mole. In glycine-sodium hydroxide buffer at pH 9.6 containing 0.0047 molar free calcium, the molar volume of formation of whole molecules was estimated to be +120 +/- 70 cm3, corresponding to an estimated difference in partial specific volume between whole molecules and half molecules of only 1.3 (10)-4cm3/gram. The correctness of the sign of this value in glycine buffer has been verified by pressure-jump light-scattering experiments.