全文获取类型
收费全文 | 1157篇 |
免费 | 0篇 |
专业分类
化学工业 | 2篇 |
金属工艺 | 4篇 |
矿业工程 | 4篇 |
轻工业 | 1篇 |
无线电 | 1篇 |
一般工业技术 | 4篇 |
冶金工业 | 1140篇 |
自动化技术 | 1篇 |
出版年
2019年 | 1篇 |
2014年 | 1篇 |
2013年 | 2篇 |
2012年 | 2篇 |
2007年 | 1篇 |
2006年 | 1篇 |
2003年 | 1篇 |
1999年 | 4篇 |
1998年 | 272篇 |
1997年 | 203篇 |
1996年 | 161篇 |
1995年 | 85篇 |
1994年 | 73篇 |
1993年 | 59篇 |
1992年 | 13篇 |
1991年 | 13篇 |
1990年 | 1篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 7篇 |
1981年 | 10篇 |
1980年 | 18篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 70篇 |
1976年 | 122篇 |
1975年 | 3篇 |
1955年 | 2篇 |
排序方式: 共有1157条查询结果,搜索用时 15 毫秒
81.
82.
83.
84.
85.
86.
87.
TV Nasedkina AE Kuz'minova SA Surkov NM Pleskach VV Prokof'eva IM Spivak VM Mikhel'son AI Poletaev 《Canadian Metallurgical Quarterly》1997,39(9):809-821
Lymphoblastoid cell lines from patients with xeroderma pigmentosum (2 forms) and progeria (unusual form) were established using transformation of peripheral blood lymphocytes by Epstein--Barr virus. The influence of different UV doses on cell vitality, proliferation and cell cycle progression was studied by means of flow cytometry. The cell vitality was determined after incubation of cells with etidium bromide and FDA. We used cytograms with two logarithmic signals (log green/log red) to discriminate the cell cycle status. Cell cultures were used with density of 500,000 cells per 1 ml, previously synchronized at G-phase by the incubation in a medium with low serum content. The effect of UV irradiation was followed during 72 h. Among four analysed cell lines only line XP2SP demonstrated enhanced UV sensitivity, expressed by decreasing of the amount of living cells after the UV dose of 2.5 J/m2 and higher. The cell cycle studies showed that cells were blocked in S-phase and simultaneously the amount of apoptotic cells with both reduced DNA content and ability to bind FDA was seen increased. Similar events were observed in the control line only after the dose of 20 J/m2 and higher. 相似文献
88.
DB Veprintsev SE Permyakov EA Permyakov VV Rogov KM Cawthern LJ Berliner 《Canadian Metallurgical Quarterly》1997,412(3):625-628
The thermal denaturation of bovine and human apo-alpha-lactalbumins at neutral pH has been studied by intrinsic protein fluorescence, circular dichroism (CD), and differential scanning microcalorimetry (DSC) methods. Apo-alpha-lactalbumin possesses a thermal transition with a midpoint about 25-30 degrees C under these conditions (pH 8.1, 10 mM borate, 1 mM EGTA), which is reflected in changes in both fluorescence emission maximum and quantum yield. However, the CD showed a decrease in ellipticity at 270 nm with a midpoint at about 10-15 degrees C, while DSC shows the transition within the region of 15-20 degrees C. The non-coincidence of transition monitored by different methods suggests the existence of an intermediate state in the course of the thermal denaturation process. This intermediate state is not the classical molten globule state which occurs at higher temperature (i.e. denatured state at these conditions) [D.A. Dolgikh, R.I. Gilmanshin, E.V. Brazhnikov, V.E. Bychkova, G.V. Semisotnov, S.Y. Venyaminov and O.B. Ptitsyn, FEBS Letters, 136 (1981) 311-315] and has physical properties intermediate between the native and molten globule states. 相似文献
89.
90.