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141.
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SM VanRheenen X Cao VV Lupashin C Barlowe MG Waters 《Canadian Metallurgical Quarterly》1998,141(5):1107-1119
SEC35 was identified in a novel screen for temperature-sensitive mutants in the secretory pathway of the yeast Saccharomyces cerevisiae (. Genetics. 142:393-406). At the restrictive temperature, the sec35-1 strain exhibits a transport block between the ER and the Golgi apparatus and accumulates numerous vesicles. SEC35 encodes a novel cytosolic protein of 32 kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec35-1 is efficiently suppressed by YPT1, which encodes the rab-like GTPase required early in the secretory pathway, or by SLY1-20, which encodes a dominant form of the ER to Golgi target -SNARE-associated protein Sly1p. Weaker suppression is evident upon overexpression of genes encoding the vesicle-SNAREs SEC22, BET1, or YKT6. The cold-sensitive lethality that results from deleting SEC35 is suppressed by YPT1 or SLY1-20. These genetic relationships suggest that Sec35p acts upstream of, or in conjunction with, Ypt1p and Sly1p as was previously found for Uso1p. Using a cell-free assay that measures distinct steps in vesicle transport from the ER to the Golgi, we find Sec35p is required for a vesicle docking stage catalyzed by Uso1p. These genetic and biochemical results suggest Sec35p acts with Uso1p to dock ER-derived vesicles to the Golgi complex. 相似文献
143.
NE Ordian AI Va?do VV Rakitskaia NV Shiriaeva FI Pro?mina NG Lopatina VG Shaliapina 《Canadian Metallurgical Quarterly》1998,125(4):443-445
Although behavioral and neuropsychological data regarding the existence of images for odors are inconclusive, reconsideration of earlier EEG work provides reasonably clear evidence for an inner nose. However, further EEG studies and neuroimaging data seem essential for conclusive demonstration of an inner nose. 相似文献
144.
Workers engaged into subsurface extraction of sand containing gold were proved to work in difficult conditions characterized by stable negative temperatures, high concentrations of dust, elevated levels of noise and vibration. The drill operators demonstrated extremely unfavorable changes of physiologic processes within a shift. Those changes were compromised cardiovascular regulation, early occurrence and intensive progress of fatigue. The authors provide recommendations to normalize the work conditions and to create rational scheme of work and rest for the occupations. 相似文献
145.
While complex examination of patients with megaureter in late stages it was established the afection in the first place of the ureter's muscular layer and the disorder of its contractice capacity. The causes of megaureter's occurrence were: obstruction of the ureter's intramural portion and vesico-ureteric reflux. Longitudinal resection of megaureter was conducted in 25 patients, the longitudinal ureter's fold formation--in 6, intraureteric plasty was done in 28. In 93% of observations the intraureteric plasty efficacy was noted. 相似文献
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In order to validate a rodent biochemical model of delayed neurotoxicity of organophosphates (OP) inhibition of rat and hen brain neurotoxic esterase (NTE) by some dichlorovinyl phosphates and phosphonates was studied in vitro and in vivo. It was shown that compounds investigated exhibited the similar inhibitory potency to NTE from both species in vitro, in addition rat and hen NTE showed the same sensitivity to variation of the structure of OP inhibitors. A good correlation was found between pI50 estimated with enzymes from rat and hen trains: r2 = 0.951, n = 18, p < or = 0.05. NTE activities were also measured in rat and hen brains after acute administration of various dosages of potent axonopathic compound dipropyldichlorovinyl phosphate. The results obtained indicate that difference in species susceptibility to neurotoxic action of OP, in particular the absence of ataxia in rats, is not caused by difference in target enzyme sensitivity to axonopathic organophosphates. 相似文献
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