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IM Rabinovich IV Bezrukova VV Khazanova EA Zemskaia NA Dmitrieva 《Canadian Metallurgical Quarterly》1997,76(4):12-17
Fifty-two patients with lichen planus were examined over the course of disease using clinical, microbiological, and histological methods. Treatment protocols aimed at normalization of microbiocenosis have been developed, based on the detected microbiological and histological changes. 相似文献
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TV Nasedkina AE Kuz'minova SA Surkov NM Pleskach VV Prokof'eva IM Spivak VM Mikhel'son AI Poletaev 《Canadian Metallurgical Quarterly》1997,39(9):809-821
Lymphoblastoid cell lines from patients with xeroderma pigmentosum (2 forms) and progeria (unusual form) were established using transformation of peripheral blood lymphocytes by Epstein--Barr virus. The influence of different UV doses on cell vitality, proliferation and cell cycle progression was studied by means of flow cytometry. The cell vitality was determined after incubation of cells with etidium bromide and FDA. We used cytograms with two logarithmic signals (log green/log red) to discriminate the cell cycle status. Cell cultures were used with density of 500,000 cells per 1 ml, previously synchronized at G-phase by the incubation in a medium with low serum content. The effect of UV irradiation was followed during 72 h. Among four analysed cell lines only line XP2SP demonstrated enhanced UV sensitivity, expressed by decreasing of the amount of living cells after the UV dose of 2.5 J/m2 and higher. The cell cycle studies showed that cells were blocked in S-phase and simultaneously the amount of apoptotic cells with both reduced DNA content and ability to bind FDA was seen increased. Similar events were observed in the control line only after the dose of 20 J/m2 and higher. 相似文献