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61.
J. I. Velasco M. Antunes O. Ayyad C. Saiz‐Arroyo M. A. Rodríguez‐Pérez F. Hidalgo J. A. de Saja 《应用聚合物科学杂志》2007,105(3):1658-1667
Novel polymer nanocomposite foams made by a two step compression molding method are analyzed in this article. Nanocomposites of low density polyethylene and an organo‐modified hectorite were first melt compounded and then foamed using a compression molding method. To study the influence of the presence and the amount of hectorite in both mechanical and thermal properties, samples with 3% and 7% content of hectorite were prepared. Polyethylene crystalline characteristics and thermal stability of the samples were studied by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA), respectively. Mechanical properties of foams and solid nanocomposites were analyzed by using dynamical mechanical analysis (DMA). Thermal expansion of the samples was analyzed by thermomechanical analysis. The results indicate that the exfoliation of hectorite platelets was achieved after the foaming process, but not during the melt mixing step. Foams with hectorite nanoparticles exhibit improved thermal stability and mechanical properties when compared with neat polymeric foams. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007 相似文献
62.
Lombó F Velasco A Castro A de la Calle F Braña AF Sánchez-Puelles JM Méndez C Salas JA 《Chembiochem : a European journal of chemical biology》2006,7(2):366-376
Thiocoraline is a thiodepsipeptide antitumor compound produced by two actinomycetes Micromonospora sp. ACM2-092 and Micromonospora sp. ML1, isolated from two marine invertebrates (a soft coral and a mollusc) found of the Indian Ocean coast of Mozambique. By using oligoprimers derived from nonribosomal peptide synthetase (NRPS) consensus sequences, six PCR fragments containing putative NRPS adenylation domains were amplified from the chromosome of Micromonospora sp. ML1. Insertional inactivation of each adenylation domain showed that two of them generated nonproducing mutants, thereby indicating that these domains were involved in thiocoraline biosynthesis. Sequencing of a 64.6 kbp DNA region revealed the presence of 36 complete open reading frames (ORFs) and two incomplete ones. Heterologous expression of a region of about 53 kbp, containing 26 of the ORFs, in Streptomyces albus and S. lividans led to the production of thiocoraline in these streptomycetes. Surprisingly, the identified gene cluster contains more NRPS modules than expected on the basis of the number of amino acids of thiocoraline. TioR and TioS would most probably constitute the NRPS involved in the biosynthesis of the thiocoraline backbone, according to the colinearity of the respective modules. It is proposed that two other NRPSs, TioY and TioZ, could be responsible for the biosynthesis of a small peptide molecule which could be involved in regulation of the biosynthesis of thicoraline in Micromonospora sp. ML1. In addition, a pathway is proposed for the biosynthesis of the unusual starter unit, 3-hydroxy-quinaldic acid. 相似文献
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Olimpio Montero Marta Velasco David Rodríguez-Lázaro Marta Hernández 《Food Analytical Methods》2013,6(6):1497-1507
Lyophylized (L) and nonlyophylized (NL) samples of two cheeses from different batches were extracted with either methanol:water (8:2, v/v) or isopropanol:water (7:3, v/v), and the extracts were analyzed by ultraperformance liquid chromatography coupled with electrospray ionization/quadruple-time-of-flight mass spectrometry (UPLC–ESI–QToF-MS) in both positive and negative modes. Chromatographic and mass spectrometric data were further compared for different samples by paired t test and principal component analysis (PCA). Mass spectra at different elution times were manually checked and major peaks tentatively identified according to their m/z values by search in available databases. A number of gangliosides and polyglycosilated ceramides were found to be the most abundant components. PCA showed samples could be clustered according to extraction procedure and cheese, and paired t test comparison also showed significant (p?<?0.05) differences between L and NL samples extracted with methanol:water. L and NL samples could only be distinguished in PCA when data from the whole chromatographic range were used. Results are discussed based on extraction solvent polarity and feasibility of PCA for comparison of cheese chemical composition after untargeted UPLC–MS analysis. 相似文献
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Tamariz E Wan AC Pek YS Giordano M Hernández-Padrón G Varela-Echavarría A Velasco I Castaño VM 《Journal of materials science. Materials in medicine》2011,22(9):2097-2109
Chemotropic proteins guide neuronal projections to their final target during embryo development and are useful to guide axons
of neurons used in transplantation therapies. Site-specific delivery of the proteins however is needed for their application
in the brain to avoid degradation and pleiotropic affects. In the present study we report the use of Poly (ethylene glycol)-Silica
(PEG-Si) nanocomposite gel with thixotropic properties that make it injectable and suitable for delivery of the chemotropic
protein semaphorin 3A. PEG-Si gel forms a functional gradient of semaphorin that enhances axon outgrowth of dopaminergic neurons
from rat embryos or differentiated from stem cells in culture. It is not cytotoxic and its properties allowed its injection
into the striatum without inflammatory response in the short term. Long term implantation however led to an increase in macrophages
and glial cells. The inflammatory response could have resulted from non-degraded silica particles, as observed in biodegradation
assays. 相似文献
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