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241.
Verena C. Stimberg Johan G. Bomer Iris van Uitert Albert van den Berg Séverine Le Gac 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(7):1076-1085
A microfluidic platform is reported for various experimentation schemes on cell membrane models and membrane proteins using a combination of electrical and optical measurements, including confocal microscopy. Bilayer lipid membranes (BLMs) are prepared in the device upon spontaneous and instantaneous thinning of the lipid solution in a 100‐μm dry‐etched aperture in a 12.5‐μm thick Teflon foil. Using this quasi‐automated approach, a remarkable 100% membrane formation yield is reached (including reflushing in 4% of the cases), and BLMs are stable for up to 36 h. Furthermore, the potential of this platform is demonstrated for (i) the in‐depth characterization of BLMs comprising both synthetic and natural lipids (1,2‐diphytanoyl‐sn‐glycero‐3‐phosphocholine (DPhPC) and L ‐α‐phosphatidylcholine (L ‐α‐PC)/cholesterol, respectively) in terms of seal resistance, capacitance, surface area, specific capacitance, and membrane hydrophobic thickness; (ii) confocal microscopy imaging of phase separation in sphingomyelin/L‐α‐PC/cholesterol ternary membranes; (iii) electrical measurements of individual nanopores (α‐hemolysin, gramicidin); and (iv) indirect assessment of the alteration of membrane properties upon exposure to chemical stimuli using the natural nanopore gramicidin as a sensor. 相似文献
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Verena Wolf Christel Baier Mila Majster-Cederbaum 《Electronic Notes in Theoretical Computer Science》2006,164(3):187-204
This paper discusses trace semantics of interactive Markov chains (IMCs) that are a generalisation of continuous-time Markov chains. In IMCs besides probabilistic alternatives there may be a nondeterministic choice between several action-labelled transitions in a state. We analyse several variants of trace equivalences that arise from the different ways one has to resolve nondeterministic branching. Button pushing testing scenarios are used to motivate each abstraction level induced by the trace semantics and associated equivalences are sorted according to their distinguishing power. 相似文献
246.
Verena Pfaffinger Ella Staufer Christian Edtmaier Aurel Arnoldt Martin Schmitz-Niederau Jelena Horky Thomas Klein 《Advanced Engineering Materials》2023,25(16):2300177
Titanium alloys gain increasing importance in industry due to the expansion of advanced manufacturing technologies such as additive manufacturing. Conventional titanium alloys processed by such technologies suffer from formation of large primary grains and anisotropy of mechanical properties. Therefore, novel alloys are required. Herein, the effect of ternary alloying elements Fe and Cr on the Ti–6.4(wt%)Ni eutectoid system is investigated. Both elements act as eutectoid formers. Fe and Cr show sluggish transformation behavior, whereas Ni is an active eutectoid-forming element. Thereby, sluggish refers to slow and active to fast transformation kinetics. The focus of this work is on the combined addition of such elements studied under different heat-treatment conditions. It is shown in the results that largely varying microstructures can be generated resulting in hardness values ranging from 239 to 556 HV0.1. Moreover, the formation of a substructure within the α phase of direct aged alloys is observed. The formation mechanism of this substructure is investigated in detail. The mechanical properties are discussed based on the microstructural characteristics. The presence of intermetallic Ti2Ni phase increases the Young's modulus, whereas the presence of ω phase results in embrittlement. The results shed light upon the complex phase formation and decomposition behavior of titanium alloys based on Ti–6.4Ni. 相似文献
247.
Christian Bartelheimer Verena Wolf Daniel Beverungen 《Information Systems Journal》2023,33(5):1085-1150
Workarounds are goal-driven deviations from the standard operating procedures performed to overcome obstacles constraining day-to-day work. Despite starting as temporary fixes, they can become established across an organisation and trigger the innovation of processes and IT artefacts that can resolve misfits permanently. Although prior research has elicited antecedents and types of workarounds, it is not known how workarounds diffuse in an organisation and, thereby, innovating co-workers' activities, IT artefacts, and organisational structures. The results of our multiple two-year case study provide unique empirical insights into the diffusion of workarounds and how they can act as generative mechanisms for bottom-up process innovation. 相似文献
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Jana Kierdorf Laura Verena Junker-Frohn Mike Delaney Mariele Donoso Olave Andreas Burkart Hannah Jaenicke Onno Muller Uwe Rascher Ribana Roscher 《野外机器人技术杂志》2023,40(2):173-192
In this paper, we present GrowliFlower, a georeferenced, image-based unmanned aerial vehicle time-series dataset of two monitored cauliflower fields (0.39 and 0.60 ha) acquired in 2 years, 2020 and 2021. The proposed dataset contains RGB and multispectral orthophotos with coordinates of approximately 14,000 individual cauliflower plants. The coordinates enable the extraction of complete and incomplete time-series of image patches showing individual plants. The dataset contains the collected phenotypic traits of 740 plants, including the developmental stage and plant and cauliflower size. The harvestable product is completely covered by leaves, thus, plant IDs and coordinates are provided to extract image pairs of plants pre- and post-defoliation. In addition, to facilitate classification, detection, segmentation, instance segmentation, and other similar computer vision tasks, the proposed dataset contains pixel-accurate leaf and plant instance segmentations, as well as stem annotations. The proposed dataset was created to facilitate the development and evaluation of various machine-learning approaches. It focuses on the analysis of growth and development of cauliflower and the derivation of phenotypic traits to advance automation in agriculture. Two baseline results of instance segmentation tasks at the plant and leaf level based on labeled instance segmentation data are presented. The complete GrowliFlower dataset is publicly available ( http://rs.ipb.uni-bonn.de/data/growliflower/ ). 相似文献
249.
Demographic change towards an ever aging population entails an increasing demand for specialized transportation systems to complement the traditional public means of transportation. Typically, users place transportation requests, specifying a pickup and a drop off location and a fleet of minibuses or taxis is used to serve these requests. The underlying optimization problem can be modeled as a dial-a-ride problem. In the dial-a-ride problem considered in this paper, total routing costs are minimized while respecting time window, maximum user ride time, maximum route duration, and vehicle capacity restrictions. We propose a hybrid column generation and large neighborhood search algorithm and compare different hybridization strategies on a set of benchmark instances from the literature. 相似文献
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