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81.
82.
Günther Raspotnig Verena Leutgeb Miriam Schaider Christian Komposch 《Journal of chemical ecology》2010,36(2):158-162
Extracts of Paranemastoma quadripunctatum (Opiliones, Dyspnoi, Nemastomatidae) contained seven components, all of which likely originated from the secretion of well-developed
prosomal scent glands. The two main components (together accounting for more than 90% of the secretion) were identified as
1,4-naphthoquinone and 6-methyl-1,4-naphthoquinone. The minor components were 1,4-naphthalenediol, two methoxy-naphthoquinones
(2-methoxy-1,4-naphthoquinone, and 2-methoxy-6-methyl-1,4-naphthoquinone) and two anthraquinones (2-methyl-9,10-anthraquinone
and a dimethyl-9,10-anthraquinone). While some chemical data on scent gland secretions of the other suborders of Opiliones
(Cyphophthalmi, palpatorean Eupnoi, and Laniatores) already exist, this is the first report on the scent gland chemistry in
the Dyspnoi. Naphthoquinones are known scent gland exudates of Cyphophthalmi and certain Eupnoi, methoxy-naphthoquinones and
anthraquinones are new for opilionid scent gland secretions. 相似文献
83.
Polymeric and porous ceramic micro- and nanospheres were prepared by a simple solidification process with a liquid preceramic polymer in an oil–water-emulsification process. The system was composed of a water-crosslinkable poly(methoxymethylsiloxane) as the preceramic polymer and a linear hydroxyl-terminated poly(dimethylsiloxane) as a source for in situ water generation. Emulsification followed by subsequent curing was carried out via an ultrasonic treatment in the presence of surfactants and a crosslinking active organometallic catalyst. The rheological behavior of the polymeric base system was characterized and the crosslinking behavior was investigated as a function of catalyst concentration and temperature. Effects of composition and processing parameters such as energy input and type and concentration of the surfactant were determined with respect to the microstructure and particle size distribution of the obtained polymeric spheres. The particle diameters of the as-synthesized spheres were mainly in the sub-micron range. Pyrolysis was performed in argon atmosphere at 1073.15 K resulting in the formation of amorphous ceramics under shape retention. 相似文献
84.
Ann-Katrin Holik Kerstin Schweiger Verena Stoeger Barbara Lieder Angelika Reiner Muhammet Zopun Julia K. Hoi Nicole Kretschy Mark M. Somoza Stephan Kriwanek Marc Pignitter Veronika Somoza 《International journal of molecular sciences》2021,22(11)
Among mammals, serotonin is predominantly found in the gastrointestinal tract, where it has been shown to participate in pathway-regulating satiation. For the stomach, vascular serotonin release induced by gastric distension is thought to chiefly contribute to satiation after food intake. However, little information is available on the capability of gastric cells to synthesize, release and respond to serotonin by functional changes of mechanisms regulating gastric acid secretion. We investigated whether human gastric cells are capable of serotonin synthesis and release. First, HGT-1 cells, derived from a human adenocarcinoma of the stomach, and human stomach specimens were immunostained positive for serotonin. In HGT-1 cells, incubation with the tryptophan hydroxylase inhibitor p-chlorophenylalanine reduced the mean serotonin-induced fluorescence signal intensity by 27%. Serotonin release of 147 ± 18%, compared to control HGT-1 cells (set to 100%) was demonstrated after treatment with 30 mM of the satiating amino acid L-Arg. Granisetron, a 5-HT3 receptor antagonist, reduced this L-Arg-induced serotonin release, as well as L-Arg-induced proton secretion. Similarly to the in vitro experiment, human antrum samples released serotonin upon incubation with 10 mM L-Arg. Overall, our data suggest that human parietal cells in culture, as well as from the gastric antrum, synthesize serotonin and release it after treatment with L-Arg via an HTR3-related mechanism. Moreover, we suggest not only gastric distension but also gastric acid secretion to result in peripheral serotonin release. 相似文献
85.
86.
Siavash Partow Verena Siewers Sara Bjørn Jens Nielsen Jérôme Maury 《Yeast (Chichester, England)》2010,27(11):955-964
The widely used pESC vector series (Stratagene, La Jolla, CA, USA) with the bidirectional GAL1/GAL10 promoter provides the possibility of simultaneously expressing two different genes from a single vector in Saccharomyces cerevisiae. This system can be induced by galactose and is repressed by glucose. Since S. cerevisiae prefers glucose as a carbon source, and since its growth rate is higher in glucose than in galactose‐containing media, we compared and evaluated seven different promoters expressed during growth on glucose (pTEF1, pADH1, pTPI1, pHXT7, pTDH3, pPGK1 and pPYK1) with two strong galactose‐induced promoters (pGAL1 and pGAL10), using lacZ as a reporter gene and measuring LacZ activity in batch and continuous cultivation. TEF1 and PGK1 promoters showed the most constant activity pattern at different glucose concentrations. Based on these results, we designed and constructed two new expression vectors which contain the two constitutive promoters, TEF1 and PGK1, in opposite orientation to each other. These new vectors retain all the features from the pESC–URA plasmid except that gene expression is mediated by constitutive promoters. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
87.
88.
Verena Schmid Karl F. Doerner Richard F. Hartl Juan-José Salazar-González 《Computers & Operations Research》2010,37(3):559-574
Companies in the concrete industry are facing the following scheduling problem on a daily basis: concrete produced at several plants has to be delivered at customers’ construction sites using a heterogeneous fleet of vehicles in a timely, but cost-effective manner. The distribution of ready-mixed concrete (RMC) is a highly complex problem in logistics and combinatorial optimization.This paper proposes two hybrid solution procedures for dealing with this problem. They are based on a combination of an exact algorithm and a variable neighborhood search (VNS) approach. The VNS is used at first to generate feasible solutions and is trying to further improve them. The exact method is based on a mixed integer linear programming (MILP) formulation, which is solved (after an appropriated variable fixing phase) by using a general-purpose MILP solver. An approach based on very large neighborhood search (VLNS) determines which variables are supposed to be fixed. In a sense, the approaches follows a local branching scheme. The hybrid metaheuristics are compared with the pure VNS approach and the conclusion is that the new metaheuristics outperform the VNS if applied solely. 相似文献
89.
90.
Boris Otto Erwin Folmer Verena Ebner 《Information Systems and E-Business Management》2012,10(4):571-602
Semantic Information Systems (IS) Standards play a critical role in the development of the networked economy. While their importance is undoubted by all stakeholders—such as businesses, policy makers, researchers, developers—the current state of research leaves a number of questions unaddressed. Terminological confusion exists around the notions of “business semantics”, “business-to-business interoperability”, and “interoperability standards” amongst others. And, moreover, a comprehensive understanding about the characteristics of Semantic IS Standards is missing. The paper addresses this gap in literature by developing a characteristics framework for Semantic IS Standards. Two case studies are used to check the applicability of the framework in a “real-life” context. The framework lays the foundation for future research in an important field of the IS discipline and supports practitioners in their efforts to analyze, compare, and evaluate Semantic IS Standards. 相似文献