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51.
Liposome-cell interaction: transfer and intracellular release of a trapped fluorescent marker 总被引:4,自引:0,他引:4
JN Weinstein S Yoshikami P Henkart R Blumenthal WA Hagins 《Canadian Metallurgical Quarterly》1977,195(4277):489-492
When small, unilamellar lipid vesicles containing a high concentration of the fluorescent dye 6-carboxyfluorescein are incubated with either frog retinas or human lymphocytes, fluroescence distributes widely throughout each cell. Since "self-quenching" largely prevents the dye from fluorescing as long as it remains sequestered in vesicles, it is clear that a considerable amount of dye is released from the vesicles and diluted into the much larger volume of the cell. 相似文献
52.
In this work, a first approach to a robust phoneme recognition task by means of a biologically inspired feature extraction method is presented. The proposed technique provides an approximation to the speech signal representation at the auditory cortical level. It is based on an optimal dictionary of atoms, estimated from auditory spectrograms, and the Matching Pursuit algorithm to approximate the cortical activations. This provides a sparse coding with intrinsic noise robustness, which can be therefore exploited when using the system in adverse environments. The recognition task consisted in the classification of a set of 5 easily confused English phonemes, in both clean and noisy conditions. Multilayer perceptrons were trained as classifiers and the performance was compared to other classic and robust parameterizations: the auditory spectrogram, a probabilistic optimum filtering on Mel frequency cepstral coefficients and the perceptual linear prediction coefficients. Results showed a significant improvement in the recognition rate of clean and noisy phonemes by the cortical representation over these other parameterizations. 相似文献
53.
A progressive fibrous myopathy may result from chronic intramuscular drug abuse. This complication may mimic other rheumatic disorders and early recognition may prevent disability. The patient described here presented with fixed flexion and extension contracture of hips and knees, respectively, after abusing meperidine and other agents for 3 years. Soft tissues of thighs and buttocks were "wood hard," EMG showed absence of action potentials in affected muscles, and biopsy revealed extensive replacement of muscle with dense, acellular fibrous tissue. Possible mechanisms are discussed. 相似文献
54.
首先通过体外模拟唾液-胃液-肠液应激实验研究乳杆菌的耐消化应激能力,然后研究其对肠道黏蛋白和Caco-2细胞的黏附及抑制肠道病原菌黏附的能力,最后探讨消化应激对乳杆菌黏附能力的影响。结果表明,副干酪乳杆菌W125、m111和发酵乳杆菌146在依次经过模拟唾液-胃液-肠液应激后存活率分别为2.70%、3.53%及11.15%,活菌数分别为7.46、7.24(lg(CFU/mL))及8.35(lg(CFU/mL)),且对黏蛋白和Caco-2细胞的黏附率显著高于其他菌株(P<0.05),分别为15.67%、8.75%、8.38%和11.47%、21.34%、10.44%;3株菌株均可通过排除、竞争和替代的方式抑制大肠杆菌CICC10899和沙门菌WX29对肠道的黏附,黏附抑制率均大于13.51%;消化应激显著降低了副干酪乳杆菌W125和发酵乳杆菌146对肠道的黏附能力(P<0.05),但显著增加了副干酪乳杆菌m111的黏附能力(P<0.05),黏附率由17.60%增加到30.45%,且主要黏附素由消化应激前的表层蛋白变为应激后的蛋白和多糖;消化应激前后副干酪乳杆菌m111均... 相似文献
55.
S. A. Khaddaj H. Al-Bahadili A. J. H. Goddard C. R. E. de Oliveira J. Wood 《Concurrency and Computation》1991,3(4):423-434
Monte Carlo and finite element methods are numerical techniques used for solving complex problems in reactor physics and radiation shielding. In this paper we are concerned with the implementation of existing research-level codes based on these two methods, written originally for serial computers, on an MIMD transputer-based system. Results and performance of the parallelized codes are presented. 相似文献
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59.
为了阐明磨石研磨加工层对高碳铬轴承钢JIS SUJ2超长寿命疲劳行为的影响,分别使用经砂纸研磨和电解研磨的砂漏形试样,在室温空气环境下进行旋转弯曲疲劳试验.砂纸研磨试样被除去部分磨石研磨层,电解研磨试样被除去了全部的磨石研磨层.结果表明,两种试样的S-N曲线由位于短寿命区的表面破坏模式和位于长寿命区的内部破坏模式的两条组成,表面破坏模式的S-N曲线受表面粗糙度和表面压缩残余应力的影响.内部破坏模式的S-N曲线不受表面条件的影响,是材料固有的特性.砂纸研磨试样表面破坏模式的疲劳极限最高,是电解研磨试样1.11倍和磨石研磨的1.20倍.表面压缩残余应力对表面破坏模式疲劳极限的影响可以用修正Goodman图表示.还讨论裂纹的萌生和扩展条件,推定超长寿命的疲劳极限. 相似文献
60.
An empirically valid outcome in psychology may differ in the degree to which the outcome does or does not conform to human intuition. The author provides a brief history of three psychological outcomes violating human intuition, notes the resemblance to the common sense revolution, and then discusses how human intuition may be detrimental to behaviorism and evolutionary perspectives of human nature. The role of human intuition in supernatural beliefs is considered and, finally, possible methods to improve the plausibility of counterintuitive outcomes are suggested. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献