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81.
82.
WB Strong 《Canadian Metallurgical Quarterly》1999,20(1):1-3; discussion 89
83.
Soluble factors from serum such as lysophosphatidic acid (LPA) are thought to activate the small GTP-binding protein Rho based on their ability to induce actin stress fibers and focal adhesions in a Rho-dependent manner. Cell adhesion to extracellular matrices (ECM) has also been proposed to activate Rho, but this point has been controversial due to the difficulty of distinguishing changes in Rho activity from the structural contributions of ECM to the formation of focal adhesions. To address these questions, we established an assay for GTP-bound cellular Rho. Plating Swiss 3T3 cells on fibronectin-coated dishes elicited a transient inhibition of Rho, followed by a phase of Rho activation. The activation phase was greatly enhanced by serum. In serum-starved adherent cells, LPA induced transient Rho activation, whereas in suspended cells Rho activation was sustained. Furthermore, suspended cells showed higher Rho activity than adherent cells in the presence of serum. These data indicate the existence of an adhesion-dependent negative-feedback loop. We also observed that both cytochalasin D and colchicine trigger Rho activation despite their opposite effects on stress fibers and focal adhesions. Our results show that ECM, cytoskeletal structures and soluble factors all contribute to regulation of Rho activity. 相似文献
84.
C. J. Barclay S. W. Campbell A. M. Galloway N. A. McPherson 《The International Journal of Advanced Manufacturing Technology》2013,65(1-4):127-140
Field activated sintering is receiving a growing attention as an alternative to classical sintering for its unique ability to realize high level of densification at low temperatures and using short processing times. This technique, however, is characterized by the presence of complex temperature and stress distributions and, as a result, microstructural non-uniformities may be produced in the samples. These temperature non-homogeneities depend on a large number of experimental parameters, such as die geometry, heating rate, material properties, sintering temperature, etc. In this paper, the various experimental parameters are systematically analyzed in order to evidence their role in determining the temperature distribution. Methods to control or remove temperature inhomogeneities will be discussed, particularly in the case of large samples. 相似文献
85.
David R. Judi Steven J. Burian Timothy N. McPherson 《Canadian Metallurgical Quarterly》2011,25(3):184-191
Emergency flood management is enhanced by using models that can estimate the timing and location of flooding. Typically, flood routing and inundation prediction is accomplished by using one-dimensional (1D) models. These have been the models of choice because they are computationally simple and quick. However, these models do not adequately represent the complex physical processes present for shallow flows located in the floodplain or in urban areas. Two-dimensional (2D) models developed on the basis of the full hydrodynamic equations can be used to represent the complex flow phenomena that exist in the floodplain and are, therefore, recommended by the National Research Council for increased use in flood analysis studies. The major limitation of these models is the increased computational cost. Two-dimensional flood models are prime candidates for parallel computing, but traditional methods/equipment (e.g., message passing paradigm) are more complex in terms of code refactoring and hardware setup. In addition, these hardware systems may not be available or accessible to modelers conducting flood analyses. This paper presents a 2D flood model that implements multithreading for use on now-prevalent multicore computers. This desktop parallel computing architecture has been shown to decrease computation time by 14 times on a 16-processor computer and, when coupled with a wet cell tracking algorithm, has been shown to decrease computation by as much as 310 times. These accomplishments make high-fidelity flood modeling more feasible for flood inundation studies using readily available desktop computers. 相似文献
86.
A molecular physics-based complementary model, which includes both field and current, is introduced to help resolve the E versus 1/E TDDB model controversy that has existed for many years It is shown here that either TDDB model can be valid for certain specified field, temperature, and molecular bonding-energy ranges. For bond strengths < 3eV, the bond breakage rate is generally dominated by field-enhanced thermal processes at lower fields and elevated temperatures and the E-Model is valid. At higher fields, lower temperatures and higher bond strengths the bond breakage mechanism must be hole-catalyzed and the TDDB physics is described well by the 1/E - model.However, neither the simple E-model nor 1/E-model works well for oxide thickness below tox< 4 run where direct tunneling effects dominate. 相似文献
87.
88.
Significant spinal deformity is particularly common in nonambulatory patients with myelodysplasia. Progressive deformity may be caused by congenital anomalies, paralytic collapse, hip contractures, or spinal cord tethering. Existing or projected functional impairment should be the principle indication for treatment. Surgical treatment is complicated by poor soft tissue coverage, associated contractures, lack of sensation, weak bone, and absence of posterior elements. Successful fusion can be achieved by circumferential (anterior and posterior) fusion and current rigid segmental instrumentation. The unique deformities and bony anatomy require individualized techniques to achieve fixation. 相似文献
89.
90.
LA Smith DE Barker CW Chase LB Somberg WB Brock RP Burns 《Canadian Metallurgical Quarterly》1997,63(12):1102-7; discussion 1107-8
The purpose of this review is to present a 4-year experience with the vacuum pack technique of temporary abdominal closure. From April 1992 to December 1996, 171 vacuum packs were performed on 93 patients. Eighty-seven vacuum packs were performed on 38 general surgical patients, and 84 vacuum packs were performed on 55 trauma patients. Overall hospital mortality was 32 per cent. Methods of achieving permanent wound closure varied in 73 patients. Four patients (4.3%) developed enterocutaneous fistulae; four patients developed intra-abdominal abscesses (4.3%). There were no eviscerations. Management of the complicated intra-abdominal process is discussed: 1) the decision to manage the abdomen in an open fashion; 2) which method of temporary closure to use; 3) subsequent explorations; 4) when the abdomen should be closed; 5) which type of closure to use; and 6) when the abdominal wall should be revised (herniorrhaphy). The vacuum pack is the method of choice for open abdomen management and temporary abdominal closure at our institution. With careful subsequent management, good patient outcome can be achieved. 相似文献