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51.
Lipoprotein lipase regulates the hydrolysis of circulating triglyceride and the uptake of fatty acids by most tissues, including the mammary gland and adipose tissue. Thus, lipoprotein lipase is critical for the uptake and secretion of the long-chain fatty acids in milk and for the assimilation of a high-fat milk diet by suckling young. In the lactating female, lipoprotein lipase appears to be regulated such that levels in adipose tissue are almost completely depressed while those in the mammary gland are high. Thus, circulating fatty acids are directed to the mammary gland for milk fat production. Phocid seals serve as excellent models in the study of lipoprotein lipase and fat transfer during lactation because mothers may fast completely while secreting large quantities of high fat milks and pups deposit large amounts of fat as blubber. We measured pup body composition and milk fat intake by isotope (deuterium oxide) dilution and plasma post-heparin lipoprotein lipase activity in six grey seal (Halichoerus grypus) mother-pup pairs at birth and again late in the 16-day lactation period. Maternal post-heparin lipoprotein lipase activity increased by an average of four-fold by late lactation (P = 0.027), which paralleled an increase in milk fat concentration (from 38 to 56%; P = 0.043). Increasing lipoprotein lipase activity was correlated with increasing milk fat output (1.3-2.1 kg fat per day) over lactation (P = 0.019). Maternal plasma triglyceride (during fasting) was inversely correlated to lipoprotein lipase activity (P = 0.027) and may be associated with the direct incorporation of long-chain fatty acids from blubber into milk. In pups, post-heparin lipoprotein lipase activity was already high at birth and increased as total body fat content (P = 0.028) and the ratio of body fat: protein increased (P = 0.036) during lactation. Although pup plasma triglyceride increased with increasing daily milk fat intake (P = 0.023), pups effectively cleared lipid from the circulation and deposited 70% of milk fat consumed throughout lactation. Lipoprotein lipase may play an important role in the mechanisms involved with the extraordinary rates of fat transfer in phocid seals. 相似文献
52.
Preliminary investigations have been made in normally hearing alert adults to establish whether the 40 Hz modulation-following response (MFR) can be used to predict 400 Hz uncomfortable loudness levels (ULLs). The MFR stimulus was a 400 Hz carrier, amplitude- and frequency-modulated by a 40 Hz sine function. Subjective ULLs were obtained using standard procedures. Objective ULLs were obtained from MFR parameter intensity functions using rms amplitude, phase angle and magnitude-squared coherence (40 Hz components). The best predictions of the subjective ULL were made using objective ULLs calculated from the gradients of linear best-fit lines for individual phase-intensity functions (80 per cent predicted within 10 dB of the subjective ULL; maximum deviation=16 dB). Poorest predictions were based on inter-subject average rms amplitude-intensity functions, where as few as 14 per cent were within 10 dB of the subjective value. The best predictions were considered sufficiently accurate to warrant further investigation using a variety of modulation and carrier frequencies in different age groups and with varying degrees of hearing loss. 相似文献
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Strohbehn John W. Yates Carter H. Curran Bruce H. Sternick Edward S. 《IEEE transactions on bio-medical engineering》1979,(5):253-262
An image processing system for image enhancement of conventional transverse axial tomography (TAT) is described. Conventional TAT means a tomography system in which the X-ray source and X-ray film move in a circular arc about the long axis of the patient, which is a different technique from computerized tomography. In the image processing system described, the original X-ray tomograms are digitized, high-pass filtered, and displayed on a TV monitor. The results of processing several clinical tomograms are shown, and definite improvements in the observed information are noted. The basic technique should be equally applicable to other conventional tomography systems, e.g., linear tomography or hypocycloidal tomography. 相似文献
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Multiple steady states are known to be possible in many types of chemical reactor due to the non-linear dependence of reaction rate on either temperature or reactant concentration. It is reasoned in this study that multiple steady states can also exist in a gas-solid fluidised bed reactor. The calculations are based on the two-phase theory of fluidisation and on the important assumption that the interstitial phase gas in completely mixed. Both thermal and concentration multiplicites are shown to be possible. 相似文献
58.
Efficiently identifying and quantifying disease- or treatment-related changes in the abundance of proteins is an important area of research for the pharmaceutical industry. Here we describe an automated, label-free method for finding differences in complex mixtures using complete LC-MS data sets, rather than subsets of extracted peaks or features. The method selectively finds statistically significant differences in the intensity of both high-abundance and low-abundance ions, accounting for the variability of measured intensities and the fact that true differences will persist in time. The method was used to compare two complex peptide mixtures with known peptide differences. This controlled experiment allowed us to assess the validity of each difference found and so to analyze the method's sensitivity and specificity. The method detects both presence versus absence and a 2-fold change in peptide concentration near the limit of detection of the instrument used, where chromatographic peaks may not be sufficiently well defined to be detected in individual samples. The method is more sensitive and gives fewer false positives than subtractive methods that ignore signal variability. Differential mass spectrometry combined with targeted MS/MS analysis of only identified differences may save both computation time and human effort compared to shotgun proteomics approaches. 相似文献
59.
Proteomic analysis of complex protein mixtures using proteolytic digestion and liquid chromatography in combination with tandem mass spectrometry is a standard approach in biological studies. Data-dependent acquisition is used to automatically acquire tandem mass spectra of peptides eluting into the mass spectrometer. In more complicated mixtures, for example, whole cell lysates, data-dependent acquisition incompletely samples among the peptide ions present rather than acquiring tandem mass spectra for all ions available. We analyzed the sampling process and developed a statistical model to accurately predict the level of sampling expected for mixtures of a specific complexity. The model also predicts how many analyses are required for saturated sampling of a complex protein mixture. For a yeast-soluble cell lysate 10 analyses are required to reach a 95% saturation level on protein identifications based on our model. The statistical model also suggests a relationship between the level of sampling observed for a protein and the relative abundance of the protein in the mixture. We demonstrate a linear dynamic range over 2 orders of magnitude by using the number of spectra (spectral sampling) acquired for each protein. 相似文献
60.
McGuire R. L. Yates E. C. Crandall D. G. Hatcher C. R. 《IEEE transactions on nuclear science》1965,12(1):24-27
A digital system has been built for making precise measurements of scintillator decay times. The approach used is similar to the one developed by Bollinger and Thomas. Time intervals between the start of a gamma-induced scintillation and the detection of a single light photon are measured with a time-to-height converter and stored in a pulse height analyzer. The apparatus has a time resolution of less than 2 nanoseconds, a dynamic range of greater than four decades, and is able to detect both prompt and slow decay components in plastic and liquid scintillators. For all fast scintillators studied, the decay of light intensity over the first decade is approximately exponential; however, measured decay times are shorter than those generally quoted. Results of measurements made on several commercially available scintillators will be presented, including NE 102, Pilot B, Pilot A, and MEL-150C. 相似文献