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51.
Ordinarily people do not make special purpose trips to acquire goods like gasoline or groceries but instead buy them as the need arises in the course of their daily lives. Such goods are commonly called convenience goods. We modify Hotelling??s model of spatial competition so that we can analyze the price equilibrium of duopolists that retail a convenience good. Certain features of the duopolists?? demand functions suggest that price competition is more severe in the convenience goods model than in the Hotelling model. The same features complicate the analysis because they mean that a pure strategy price equilibrium does not exist for many locational configurations. Although we are not able to find the mixed strategy price equilibrium analytically, we do present some numerical results on equilibrium prices that broadly confirm this suggestion. We also provide a more general product differentiation interpretation of the convenience good model. 相似文献
52.
Leonard K. Eaton 《The Journal of Architecture》2013,18(4):315-346
This paper traces the development of Frank Lloyd Wright's ideas on the place of the concrete column and slab in architecture. It deals with his first employment of the slab at Unity Temple (Oak Park, 1904) and his use of the form in residential projects during the next few years. It surveys American literature on concrete construction and briefly analyses the contribution of Claude A.P. Turner and the employment of the Turner system in two Chicago buildings that Wright would have known. It discusses his project for the San Francisco Call (1912) and the use of the Barton system in the Richland Center warehouse for A.D. German (1915). It further discusses his first probable knowledge of the more sophisticated concrete slabs of Maillart, published in 1926, and of Maillart's invention of a proto lily-pad capital. This capital is also to be seen in Wright's champagne glass designed for Leerdam (1930) and in his project for the Salem Capitol Journal of 1931. Finally it is argued that the much admired lily-pad columns at the Johnson Wax office building (Racine, 1937-39) are really a series of three-hinged bents rather than shell construction. 相似文献
53.
The jaw fat of the Atlantic bottlenose dolphin (Tursiops truncatus) contains unusual wax esters which can be separated into short chain (<C24) and long chain (>C24) fractions by thin layer chromatography. The short chain wax esters (28 wt. %) have been characterized as a 72∶24∶4 mixture
of isovaleroyl, isobutoryl, and 2-methylbutyrol, esters of C14–C18 n- and iso-alcohols. The intact <C24 esters have been resolved into individual molecular species by gas liquid chromatography on open-tubular polyester columns.
The long chain wax esters (12 wt. %) contain C10–C22 n- and iso-acids esterified to the same C14–C18 n- and iso-alcohols. Gas liquid chromatography of the intact, hydrogenated >C24 esters on a short JXR column has characterized them according to carbon number and the number of methyl branches they contain. 相似文献
54.
冷板辐射加置换通风空词系统在保证室内环境的前提下比常规全空气空调系统节能,但在天气湿热的香港地区应用会出现冷板凝露现象,且采用置换通风方式,风量有限从而影响热舒适性.本文将干式风机盘管系统与独立除湿通风系统组合应用于香港地区办公楼,干式风机盘管系统处理室内显热负荷,独立除湿通风系统承担室内湿负荷和室外全热负荷.采用EnergyPlus软件模拟分析了该空调系统在香港地区某办公楼中的使用性能,结果表明该系统能很好地控制室内温、湿度,特别是湿度,与常规全空气空调系统相比,全年节能达10.3%. 相似文献
55.
The unique characteristic of subcritical water is its widely tunable physical properties. For example, the polarity (measured by dielectric constant) of water is significantly decreased by raising water temperature. At temperatures of 200-250 °C (under moderate pressure to keep water in the liquid state), the polarity of pure water is similar to that of pure methanol or acetonitrile at ambient conditions. Therefore, pure subcritical water may be able to serve as the mobile phase for reversed-phase separations. To investigate the retention behavior in subcritical water separation, the retention factors of BTEX (benzene, toluene, ethylbenzene, and m-xylene), phenol, aniline, and their derivatives have been determined using subcritical water, methanol/water, and acetonitrile/water systems. Subcritical water separations were also performed using alumina, silica-bonded C18, and poly(styrene-divinylbenzene) columns to study the influence of the stationary phase on analyte retention under subcritical water conditions. 相似文献
56.
D Robinette S Wada T Arroll MG Levy WL Miller EJ Noga 《Canadian Metallurgical Quarterly》1998,54(5):467-475
BACKGROUND: A major reduction in the energy demand of the myocardium results from the electromechanical arrest, and cooling contributes to a lesser degree to this reduction. It is from this assumption that strategies of myocardial protection, utilizing warm blood cardioplegic induction, followed by cold cardioplegia with terminal warm reperfusion before removal of the aortic cross clamp, became established as optimal myocardial protection. Continuous normothermic perfusion 'closed the loop' by avoiding myocardial ischemia and linking warm induction and terminal reperfusion. A series of laboratory and clinical data confirmed the benefits of warm heart surgery on myocardial function and metabolism. The disadvantages of continuous warm blood cardioplegia including disturbance of the operative field, led surgeons to administer warm hyperkalaemic blood intermittently as a new cardioplegic strategy. METHODS: This review examines the laboratory and clinical data with reference to the intermittent warm blood cardioplegia, to establish its experimental basis and place in clinical practice. CONCLUSIONS: Experimental observation and clinical application have established intermittent warm blood cardioplegia as a practical, effective and cheap myocardial protection technique, particularly with reference to coronary artery surgery. 相似文献
57.
DA Toke WL Bennett DA Dillon WI Wu X Chen DB Ostrander J Oshiro A Cremesti DR Voelker AS Fischl GM Carman 《Canadian Metallurgical Quarterly》1998,273(6):3278-3284
Diacylglycerol pyrophosphate (DGPP) is involved in a putative novel lipid signaling pathway. DGPP phosphatase (DGPP phosphohydrolase) is a membrane-associated 34-kDa enzyme from Saccharomyces cerevisiae which catalyzes the dephosphorylation of DGPP to yield phosphatidate (PA) and then catalyzes the dephosphorylation of PA to yield diacylglycerol. Amino acid sequence information derived from DGPP phosphatase was used to identify and isolate the DPP1 (diacylglycerol pyrophosphate phosphatase) gene encoding the enzyme. Multicopy plasmids containing the DPP1 gene directed a 10-fold overexpression of DGPP phosphatase activity in S. cerevisiae. The heterologous expression of the S. cerevisiae DPP1 gene in Sf-9 insect cells resulted in a 500-fold overexpression of DGPP phosphatase activity over that expressed in wild-type S. cerevisiae. DGPP phosphatase possesses a Mg2+-independent PA phosphatase activity, and its expression correlated with the overexpression of DGPP phosphatase activity in S. cerevisiae and in insect cells. DGPP phosphatase was predicted to be an integral membrane protein with six transmembrane-spanning domains. The enzyme contains a novel phosphatase sequence motif found in a superfamily of phosphatases. A dpp1Delta mutant was constructed by deletion of the chromosomal copy of the DPP1 gene. The dpp1Delta mutant was viable and did not exhibit any obvious growth defects. The mutant was devoid of DGPP phosphatase activity and accumulated (4-fold) DGPP. Analysis of the mutant showed that the DPP1 gene was not responsible for all of the Mg2+-independent PA phosphatase activity in S. cerevisiae. 相似文献
58.
Previous studies of the annexin family of Ca2+ binding proteins identified a soluble monomer in the absence of Ca2+ and a trimer adsorbed on the membrane surface in the presence of Ca2+. On the basis of site-directed spin-labeling studies of annexin XII at low pH, we now report a membrane-inserted form of the protein with a dramatically different structure. The data suggest that upon insertion a continuous transmembrane alpha-helix is reversibly formed from a helix-loop-helix motif in the solution structure. Other regions with similar membrane-insertion potential were identified in the amino acid sequence, and we propose that the corresponding helices come together to form an aqueous pore that mediates the ion channel activity reported for several annexins. 相似文献
59.
BD Strahl HJ Huang J Sebastian BR Ghosh WL Miller 《Canadian Metallurgical Quarterly》1998,139(11):4455-4465
60.
Animal models of fungal and other infectious diseases often require that the number of organisms in tissue be quantified, traditionally by grinding organs, plating them on agar and counting colony forming units (CFU). This method is labor intensive, slow as some fungi require two weeks of culture and limited in reliability by poor plating efficiency. To circumvent these problems, we developed a flow cytometric method to quantify yeast. In vitro cultured Blastomyces dermatitidis, Cryptococcus neoformans, Candida albicans and Histoplasma capsulatum yeast were labelled with specific monoclonal or polyclonal antibodies to stain surface determinants or with Calcofluor to stain cell-wall chitin. A defined number of fluorescently labelled beads were added prior to acquisition by flow cytometry as a reference standard for quantitation. Beads were readily distinguished from yeast by forward scatter, side scatter and intensity of fluorescence. Cultured yeast were enumerated by both standard CFU determination and flow cytometry in a range of 10(2) to 10(7) cells. Only flow cytometry enabled discrimination of live and dead yeast by using appropriate fluorescent dyes. The flow cytometric method was applied to murine models of histoplasmosis and blastomycosis to quantify the burden of fungi in the lungs of infected mice. Labelling yeast with Calcofluor alone resulted in unacceptably high levels of nonspecific binding to mouse cell debris. In contrast, labelling H. capsulatum with a rabbit polyclonal antiserum and B. dermatitidis with a monoclonal antibody to the surface protein WI-1 permitted accurate quantitation. We conclude that this flow cytometry technique is rapid, efficient and reliable for quantifying the burden of infection in animal models of fungal disease. The technique also should lend itself to performing cytotoxicity assays that require discrimination of live and dead fungi, or phagocytosis assays that require discrimination of intracellular and extracellular organisms. 相似文献