Energy transfer in the photochemical apparatus of flashed bean leaves

Fluorescence and energy transfer properties of bean leaves greened by brief, repetitive xenon flashes were studied at -196 degrees C. The bleaching of P-700 has no influence on the yield of fluorescence at any wavelength of emission. The light-induced fluorescence yield changes which are observed in both the 690 and 730 nm emission bands in the low temperature fluorescence spectra are due to changes in the state of the Photosystem II reaction centers. The fluorescence yield changes in the 730 nm band are attributed to energy transfer from Photosystem II to Photosystem I. Such energy transfer was also confirmed by measurements of the rate of photooxidation of P-700 AT -196 degrees C in leaves in which the Photosystem II reaction centers were either all open or all closed. It is concluded that energy transfer from Photosystem II to Photosystem I occurs in the flashed bean leaves which lack the light-harvesting chlorophyll a/b protein.     

Leucocyte-migration inhibition induced by uveoretinal antigen in patients with diabetic retinopathy

Leucocyte-migration inhibition was to determine the state of hypersensitivity in 65 diabetic patients with different stages of retinopathy and 21 nondiabetic controls. About one third of the patients with simple or proliferative retinopathy exhibited significant leucocyte-migration inhibition of 0.2 mg/ml. protein concentration of uvreoretinal antigen. In contrast, only one of 15 patients with minimal retinopathy and none of the controls showed significant leucocyte-migration inhibition. Corneal and lenticular antigens did not evoke a cellular immune response in any of the tested individuals. These findings suggest that cell-mediated hypersensitivity to uveoretinal antigen may develop in diabetic patients with prolonged, progressive, simple or proliferative retinopathy.     

Frequency of the carrier state for X-linked chronic granulomatous disease among females with lupus erythematosus

Carriers for chronic granulomatous disease (CGD) and patients with lupus erythematosus (LE) share several characteristics: They are mostly females, they reduce nitroblue tetrazolium (NBT) poorly in their neutrophils, and, in some cases, they have similar skin lesions. We thus investigated 19 female LE patients for the presence of laboratory findings characteristic of the carrier state for CGD. None of these patients turned out to have the combined abnormality of neutrophil bactericidal activity and neutrophil NBT-reduction that is diagnostic of CGD carrier state in the X-linked form. An increased frequency of CGD carriers among female LE patients thus appear to be unlikely. Why some CGD carriers develop skin lesions typical of LE remains unexplained.     

A rapid and sensitive method for determination of theophylline in plasma and saliva by high pressure liquid chromatography

A fast and sensitive high pressure liquid chromatographic method for the monitoring of theophylline concentrations in plasma or saliva was developed. Each sample requires only about 15 minutes for the completion of the assay after receiving the plasma or saliva sample. Only 0.1 ml of sample is required, and concentrations as low as 1 mcg/ml can be accurately measured.     

Serial measurements of left ventricular ejection fraction by radionuclide angiography early and late after myocardial infarction

1-Acyl-2-succinyl glycero-3-phosphorylcholine (GPC) was synthesized and its properties described. Although 1-acyl-2-succinyl GPC is a good substrate for succinate dehydrogenase, experiments on the incorporation of [2,3-14C] succinate into mitochondrial lipids gave no evidence to indicate that it is an intermediate in the enzymic oxidation of succinate to fumerate, as has been suggested earlier.     

Creation of reduced fat foods: Influence of calcium-induced droplet aggregation on microstructure and rheology of mixed food dispersions

The impact of calcium-induced fat droplet aggregation on the microstructure and physicochemical properties of model mixed colloidal dispersions was investigated. These systems consisted of 2 wt% whey protein-coated fat droplets and 4 wt% modified starch granules heated to induce starch swelling (pH 7). Optical and confocal microscopy showed that the fat droplets were dispersed within the interstitial region between the swollen starch granules. The structural organisation of the fat droplets within these interstitial regions could be modulated by controlling the calcium concentration: (i) at a low calcium concentration the droplets were evenly distributed; (ii) at an intermediate calcium concentration they formed a layer around the starch granules; (iii) at a high calcium concentration they formed a network of aggregated droplets. Paste-like materials were produced when the fat droplets formed a three-dimensional network in the interstitial region. The properties of fat droplet–starch granule suspensions can be modulated by altering the electrostatic interactions to alter microstructure.     

Lemon oil solubilization in mixed surfactant solutions: Rationalizing microemulsion & nanoemulsion formation

Lipophilic functional ingredients are usually incorporated into aqueous-based foods and beverages in the form of colloidal dispersions. In this study, we investigated the rate and extent of solubilization of emulsified lemon oil in mixed non-ionic surfactant solutions (buffer: propylene glycol = 2:1): sucrose monopalmitate (SMP) and/or Tween 80 (T80). The influence of surfactant concentration, type, and mixing ratio on lemon oil solubilization was investigated, with the aim of identifying suitable conditions for preparing stable microemulsions and nanoemulsions. Solubilization was monitored by measuring changes in light scattering by lemon oil droplets after they were dispersed in surfactant solutions (pH 7). The solubilization process was rapid (<few minutes), with the rate increasing with increasing surfactant concentration. For a particular surfactant type and concentration, lemon oil was transferred from nanoemulsion droplets into microemulsion droplets until a critical lemon oil concentration (C_sat) was reached, after which it remained as nanoemulsion droplets. The value of C_sat increased with increasing surfactant concentration and was higher for SMP than Tween 80. The impact of storage at pH 3.5 on the physical stability of microemulsions and nanoemulsions was examined. Acid stable colloidal dispersions could not be formed using SMP alone. However, relatively stable nanoemulsions and microemulsions could be formed when ≥75 or 50 wt% Tween 80 was incorporated into the surfactant phase, respectively. This study provides important information for the rational design of food-grade colloidal delivery systems for encapsulating and delivering functional lipids for food and beverage applications.     

Drivers of change in groundwater resources: a case study of the Indian Punjab

Indian Punjab, a strategically important region from India’s national food security standpoint, is increasingly the focus of attention for academics and policymakers because of serious concerns about over-exploitation of its groundwater resources. Currently, policy makers and agricultural researchers/scientists in India are in a fix to prescribe an alternative, probably more sustainable, crop-mix to farmers that can save water while maintaining farm incomes. Using primary data from 120 farmers, this paper evaluates the current situation of groundwater resources in Punjab, and outlines the major socio-economic factors that have a significant association with the change in the groundwater depth in this region. General ANOVA regression results suggest that groundwater depth varied significantly with respect to agro-climatic regions, crop diversity, and farmer education. Crop diversity had an inverse relationship with groundwater depth whereas the association between farmer education and groundwater depth was non-linear although in the case of Gurdaspur, they showed a direct relationship. In the central zone of Indian Punjab, groundwater level on 92% of the farms had depleted by more than 0.60 m annually between 2000 and 2010, while the current state of groundwater resources in the other two regions was not so serious and are manageable for the time being. However, if the existing policy framework for groundwater resources in the state, which allows the state government to release 0.11 million more connections to farmers, putting much pressure not only on the groundwater resources but also burdening the state exchequer, continues, Punjab may end up losing all its groundwater resources for ever. Considering this alarming situation, one-fifth of the farmers surveyd agreed to delay the sowing of rice by another 2 weeks i.e. up to 30 June which could save the fast depleting groundwater resources in Punjab.