Calciphylaxis in man. A syndrome of tissue necrosis and vascular calcification in 11 patients with chronic renal failure

Eleven patients with chronic renal failure and presumed secondary hyperparathyroidism developed a syndrome of medial calcinosis of the arteries and painful ischemic ulcers of the fingers, legs, or thighs, or any combination of the three. Five patients required maintenance hemodialysis; six had functioning renal homografts. Severe hyperphosphatemia had existed in each; seven showed roentgenographic evidence of subperiosteal resorption. Similarities are evident between the lesions and experimentally produced calciphylaxix. The lesions demonstrated a relentless, progressive course, with serious morbidity and mortality. Hyperplastic or adenomatours parathyroid tissue was removed from ten of 11 patients unergoing surgical procedures; healing followed in seven patients. Treatment with phosphate-binding antacids to lower serum phosphorus levels may prevent this syndrome. Total or subtotal parathyroidectomy should be considered when ischemic skin lesions appear in uremic patients or in renal transplant recipients.     

An approximate compositional approach to the analysis of fluid queue networks

Fluid models have for some time been used to approximate stochastic networks with discrete state. These range from traditional ‘heavy traffic’ approximations to the recent advances in bio-chemical system models. Here we present a simple approximate compositional method for analysing a network of fluid queues with Markov-modulated input processes at equilibrium. The idea is to approximate the on/off process at the output of a queue by an n-state Markov chain that modulates its rate. This chain is parameterised by matching the moments of the resulting process with those of the busy period distribution of the queue. This process is then used, in turn, as a separate Markov-modulated on/off process that feeds downstream queue(s). The moments of the busy period are derived from an exact analytical model. Approximation using two- and three-state intermediate Markov processes are validated with respect to an exact model of a tandem pair of fluid queues — a generalisation of the single queue model. The analytical models used are rather simpler and more accessible, albeit less general, than previously published models, and are also included. The approximation method is applied to various fluid queue networks and the results are validated with respect to simulation. The results show the three-state model to yield excellent approximations for mean fluid levels, even under high load.     

用微滤处理乳化含油废水

考察了ＰＶＤＦ膜和复合陶瓷膜的水通量，油截留率，以及膜堵塞等。研究结果表明：微波的水通量和油截留率均很高，透过液中油含量＜４０－２０ｍｇ／Ｌ。微波是一种很有应用前景的废水处理方法。另外，还讨论了在膜堵塞状态下，微波膜对溶于水小分子有机物的截留率。     

Precoupling of Gi/G(o)-linked receptors and its allosteric regulation by monovalent cations

The ability of receptors (R) to activate G-proteins (G) is promoted by the binding of agonists, reflecting their induction of a receptor conformation which facilitates both the formation of a RG complex and guanine nucleotide exchange. Recent evidence from isolated membrane studies has indicated, however, that some receptors have the inherent ability to form RG complexes and promote GDP/GTP exchange in their unoccupied state. These receptors preferentially activate pertussis toxin-sensitive G-proteins (i.e. Gi/G(o)) and the interactions of R and G are modulated by monovalent cations (most notably Na+) both in the unoccupied and agonist-occupied states. Basal G-protein activation by such receptors is reduced both by increasing levels of cation and by antagonists which may act by inducing receptor conformations which are less favorable for RG complexation. This behaviour conforms to the predictions of a ternary complex model of receptor function and can be considered in structural terms for those receptors such as the alpha-2 adrenergic receptor where ligand binding and G-protein activation regions have been proposed.     

Tetracaine reports a conformational change in the pore of cyclic nucleotide-gated channels

Local anesthetics are a diverse group of clinically useful compounds that act as pore blockers of both voltage- and cyclic nucleotide-gated (CNG) ion channels. We used the local anesthetic tetracaine to probe the nature of the conformational change that occurs in the pore of CNG channels during the opening allosteric transition. When applied to the intracellular side of wild-type rod CNG channels expressed in Xenopus oocytes from the alpha subunit, the local anesthetic tetracaine exhibits state-dependent block, binding with much higher affinity to closed states than to open states. Here we show that neutralization of a glutamic acid in the conserved P region (E363G) eliminated this state dependence of tetracaine block. Tetracaine blocked E363G channels with the same effectiveness at high concentrations of cGMP, when the channel spent more time open, and at low concentrations of cGMP, when the channel spent more time closed. In addition, Ni2+, which promotes the opening allosteric transition, decreased the effectiveness of tetracaine block of wild-type but not E363G channels. Similar results were obtained in a chimeric CNG channel that exhibits a more favorable opening allosteric transition. These results suggest that E363 is accessible to internal tetracaine in the closed but not the open configuration of the pore and that the conformational change that accompanies channel opening includes a change in the conformation or accessibility of E363.     

Model Selection in Applied Science and Engineering: A Decision-Theoretic Approach

Mathematical models are developed and used to study the properties of complex systems in just about every area of applied science and engineering. Information on the system being modeled is, in general, incomplete, so that there may be two or more models consistent with the available information. The collection of these models is called the class of candidate models. A decision-theoretic method is developed for selecting the optimal member from the collection. The optimal model depends on the available information, the class of candidate models, and the model use. The candidate models may be deterministic or random. Classical methods for model selection, including the method of maximum likelihood and Bayesian methods, are briefly reviewed. These methods ignore model use and require data to be available. In addition, examples are used to show that classical methods for model selection can be unreliable in the sense that they can deliver unsatisfactory models when data is limited. The proposed decision-theoretic method for model selection does not have these limitations. The method accounts for model use via a utility function. This feature is especially important when modeling high-risk systems where the consequences of using an inappropriate model for the system can be disastrous.     

Protein analysis of human von Ebner saliva and a method for its collection from the foliate papillae

The lingual serous glands of von Ebner are located close to the foliate and circumvallate papillae. Saliva secreted by these glands provides the immediate environment of the taste buds, and it has been hypothesized that it modulates taste perception. The purpose of this study was to develop a technique for collection of unstimulated and stimulated saliva from human von Ebner glands. Saliva was collected under resting conditions and after application of various gustatory stimuli (sweet, sour, salt, and bitter) by insertion of periostrips into the folds of the foliate papillae of healthy human volunteers. Stimulated saliva was also collected in glass microcapillaries or micropipettes. The flow-rates of unstimulated von Ebner saliva were 2.3 +/- 0.6 (S.E.) microL/min and 4.5 +/- 1.2 (S.E.) microL/min with 1% citric acid stimulation. The protein content was 2.5 +/- 0.5 (S.E.) mg/mL. The SDS gel electrophoretic profile of von Ebner saliva revealed two protein bands of Mr 18,000 that were identified on Western blots as von Ebner gland (VEG) proteins. Although lingual lipase activity was detected at very low levels by enzyme assay, this protein was not detected on Western blots. This collection technique should prove useful for analysis of specific functions associated with secretion from von Ebner glands.     

论水平裂纹表面的扩展条纹

本文研究水平裂纹表面的扩展条纹,讨论其特点,分析其形成。指出固体粒子冲击并脱离靶面后由于水平裂纹扩展所形成的薄碎片尚在自由振动,使水平裂纹在其扩展过程中CTOD(裂纹顶端张开位移)发生周期性改变,从而形成扩展条纹。用薄膜自由振动模型估算条纹间距,并与多种材料的实测结果进行对比,两者近似。