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81.
Park N. -J. Field D. P. Nowell M. M. Besser P. R. 《Journal of Electronic Materials》2005,34(12):1500-1508
Microstructural evolution during elevated temperature annealing of sputter deposited copper (Cu) films was investigated by
electron backscatter diffraction (EBSD). Analysis of films was performed both in situ using a heating stage, and by ex-situ
observation of microstructural evolution. It was noted that not only is the Cu film texture and grain size a function of film
thickness, but also that the fraction of twin boundaries present in the material is strongly dependent upon film thickness.
This is explained by means of a simple model that considers the energy of the system. Surface and interface energies, as well
as grain boundary energies for random high angle boundaries and for twin boundaries (both coherent and incoherent planes)
are used in the determination. The model was shown to accurately predict the twin boundary size in self-annealed films. This
type of analysis also results in a texture map similar to that presented by Thompson,12 but incorporates the development and effect of twin boundaries, so that additional texture components (in addition to 111
and 100 fibers) are included. 相似文献
82.
雷诺应力(RSM)模型对旋流器分离性能预测研究 总被引:6,自引:0,他引:6
为了优化液.液旋流管的结构设计,进行了旋流管结构筛选试验研究和旋流管内单相和油水两相湍流流动的数值模拟技术研究。应用CFD计算软件,采用雷诺应力模型对液.液旋流分离管内的单相强旋流场进行数值模拟。计算出了旋流管内的压力场分布、各旋流管压力降以及压力降在旋流管各部件的分配比例。各旋流管的压力降计算值和试验吻合较好,相对误差基本不超过10%,计算出了各种旋流管的粒级效率曲线,计算结果与试验结果一致。 相似文献
83.
M Bandell ME Lhotte C Marty-Teysset A Veyrat H Prévost V Dartois C Diviès WN Konings JS Lolkema 《Canadian Metallurgical Quarterly》1998,64(5):1594-1600
Citrate metabolism in the lactic acid bacterium Leuconostoc mesenteroides generates an electrochemical proton gradient across the membrane by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). Reports on the energetics of citrate metabolism in the related organism Lactococcus lactis are contradictory, and this study was performed to clarify this issue. Cloning of the membrane potential-generating citrate transporter (CitP) of Leuconostoc mesenteroides revealed an amino acid sequence that is almost identical to the known sequence of the CitP of Lactococcus lactis. The cloned gene was expressed in a Lactococcus lactis Cit- strain, and the gene product was functionally characterized in membrane vesicles. Uptake of citrate was counteracted by the membrane potential, and the transporter efficiently catalyzed heterologous citrate-lactate exchange. These properties are essential for generation of a membrane potential under physiological conditions and show that the Leuconostoc CitP retains its properties when it is embedded in the cytoplasmic membrane of Lactococcus lactis. Furthermore, using the same criteria and experimental approach, we demonstrated that the endogenous CitP of Lactococcus lactis has the same properties, showing that the few differences in the amino acid sequences of the CitPs of members of the two genera do not result in different catalytic mechanisms. The results strongly suggest that the energetics of citrate degradation in Lactococcus lactis and Leuconostoc mesenteroides are the same; i.e., citrate metabolism in Lactococcus lactis is a proton motive force-generating process. 相似文献
84.
Non-accidental head injury, be it shaking, impact(s) or a combination of the two, is characterised by subdural and/or subarachnoid haemorrhages with retinal haemorrhages, but minimal or absent external cranio-facial trauma. The classical assault scenario depicts the infant being gripped around the head, face, chest and abdomen and shaken or being gripped by a limb and swung. This gripping might be expected to leave physical evidence in the form of bruising. A study was undertaken to establish the prevalence, distribution and pathological association of external bruising in 24 cases of fatal non-accidental head injury in children. At autopsy, 17 cases had new external bruises, 15 old external bruises and 13, a combination of both. However, seven (29%) cases showed no fresh external bruising and five (21%) showed no external bruising at all. Thus, external bruising may be absent in children with fatal intracranial injury. The face was shown to be the commonest site of bruising followed by the forehead and buttocks. Limb, chest and abdominal bruising were found to be uncommon. Retinal haemorrhages were confirmed in 23 (96%) cases. It is hypothesised that bruising, when present, may be a result of abuse in the form of punches and slaps rather than due to gripping during the assault. We discuss why gripping does not necessarily result in external bruising. 相似文献
85.
86.
Fluorescent in situ hybridisation (FISH) is a powerful tool for the evaluation of chromosomal alterations in formalin fixed paraffin wax embedded sections of colorectal cancer. However, initial experiments using a two-step detection system for digoxigenin labelled chromosome specific centromeric probes resulted in a complete lack of hybridisation signal from a number of colorectal tumour sections. This was due to high levels of background autofluorescence observed in this tissue, which masked any relatively weak hybridisations present. To overcome this problem, a biotinylated tyramide mediated amplification system was incorporated into the FISH detection protocol. This involves the use of horseradish peroxidase to activate the biotinylated tyramide, resulting in the deposition of a large number of biotin molecules at the site of bound peroxidase, which corresponds directly to the location of hybridised probe. Final detection was by means of a streptavidin-FITC conjugate. Using this technique, a panel of 11 colorectal tumour samples studied to date have shown strong, specific hybridisation signals to the nucleus of tumour cells. Amplification of FISH signals by biotinylated tyramide has the potential to improve weak hybridisation signals in cells from numerous sources, using a variety of probe types, including single copy gene probes as well as centromere specific probes. 相似文献
87.
Elías Razo-Flores Margarita Iniestra-González Jim A. Field Patricia Olguín-Lora Laura Puig-Grajales 《Canadian Metallurgical Quarterly》2003,129(11):999-1006
The anaerobic biodegradability of mixtures of phenolic compounds was studied under continuous and batch systems. Continuous experiments were carried out in up-flow anaerobic sludge bed (UASB) reactors degrading a mixture of phenol and p-cresol as the main carbon and energy sources. The total chemical oxygen demand (COD) removal above 90% was achieved even at organic loading rates as high as 7 kg COD/m3/day. Batch experiments were conducted with mixtures of phenolic compounds (phenol, p-cresol, and o-cresol) to determine the specific biodegradation rates using unadapted and adapted anaerobic granular sludge. Phenol and p-cresol were mineralized by adapted sludge with rates several orders of magnitude higher than unadapted sludge. Additionally, an UASB reactor was operated with the mixture phenol, p-cresol, and o-cresol. After 54 days of operation, 80% of o-cresol (supplied at 132 mg/L) was eliminated. The phenol biodegradation was not affected by the presence of o-cresol. These results demonstrate that major phenolic components in petrochemical effluents can be biodegraded simultaneously during anaerobic treatment. 相似文献
88.
89.
An effective topical and regional anesthetic technique using 25% cocaine paste combined with intravenous midazolam hydrochloride was used in 554 patients undergoing functional endoscopic sinus surgery. The operative fields were excellent and all procedures were completed with little patient disturbance. Patients recovered rapidly from sedation and were usually fit for discharge on the same day. There were no anesthetic complications. The major surgical complication rate was 0.5%. 相似文献
90.
本文研究水平裂纹表面的扩展条纹,讨论其特点,分析其形成。指出固体粒子冲击并脱离靶面后由于水平裂纹扩展所形成的薄碎片尚在自由振动,使水平裂纹在其扩展过程中CTOD(裂纹顶端张开位移)发生周期性改变,从而形成扩展条纹。用薄膜自由振动模型估算条纹间距,并与多种材料的实测结果进行对比,两者近似。 相似文献