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991.
T Voit CA Sewry K Meyer R Hermann V Straub F Muntoni T Kahn R Uns?ld TR Helliwell R Appleton 《Canadian Metallurgical Quarterly》1995,26(3):148-155
The merosin M-chain (or laminin-alpha 2) is one of three subunits of laminin-2 which is highly expressed in striated muscle and peripheral nerve. Complete lack of laminin-alpha 2 expression in skeletal muscle is the hallmark of one form of congenital muscular dystrophy which is characterized by dysmyelination of the central nervous system (CNS), links to chromosome 6q2 and is common among Caucasians. Laminin-alpha 2 expression was also found to be significantly reduced in Fukuyama congenital muscular dystrophy which links to chromosome 9q3. We report consistently preserved laminin-2 expression, including laminin-alpha 2, as detected by immunofluorescence in skeletal muscle from five patients with Walker-Warburg syndrome which is characterized by congenital muscular dystrophy and, in addition, type II lissencephaly or pachygyria, defective CNS myelination, and ocular dysgenesis. These findings show that in spite of partial phenotypic overlap between Fukuyama CMD and Walker-Warburg syndrome the two disorders are nosologically separate disease entities. They also exclude that Walker-Warburg syndrome is allelic to the common form of congenital muscular dystrophy with laminin-alpha 2 deficiency. 相似文献
992.
Meyer L.H. Jayaram S.H. Cherney E.A. 《Dielectrics and Electrical Insulation, IEEE Transactions on》2005,12(6):1201-1208
The standard methods available to test tracking and erosion resistance of filled silicone rubber do not allow to delineate the fundamentals of the thermal degradation, although the heat from the dry band arcing is the main degradation factor. In this work a thermal imaging camera is used to investigate the performance of filled silicone rubber in an inclined plane test, and a scheme is established to relate the temperature and the electrical discharge energy. Further, an infrared laser technique, based on constant energy approach, is developed to study the material performance under laser heating. The technique consists of applying a certain amount of infrared laser energy during a set time period, in order to produce erosion in the silicone rubber samples. Through correlation studies the applicability of the proposed laser technique to rank silicone rubber samples with various fillers has been validated. The laser tests show identical ranking of samples when compared to samples ranked using inclined plane tests, yet offering significant advantages of being faster, simpler, and reproducible. 相似文献
993.
The tensile mechanical properties of welded titanium joints were studied, and intact titanium was used as controls. Welded joints were fabricated with either a stereographic laser-welding technique or a gas tungsten arc welding technique. The effect of heat treatment following a simulated porcelain application was also investigated. Heat-treated laser welds had significantly lower ultimate tensile strengths. Heat treatment had no effect on the modulus of elasticity or elongation, but generally significantly decreased the yield strength of the titanium specimens. The gas tungsten are welding specimens had significantly higher yield strengths and elastic moduli than the other two groups. The elongation of the control specimens was significantly greater than the elongation of the gas tungsten arc welding specimens, which was in turn significantly higher than that of the laser-welded specimens. 相似文献
994.
WT Millar GJ Van Den Aardweg JW Hopewell PA Canney 《Canadian Metallurgical Quarterly》1996,69(1):123-140
Pig skin was irradiated using 90Sr/90Y plaques and the dose-related incidence of induced moist desquamation was determined. The repair of radiation-induced sublethal damage (SLD) was studied by fitting these response data to the generalized LQ equation for incomplete repair using quasilikelihood methods with binomial statistics, and either a Poisson or logistic link to relate the probability of response to the covariates. A Poisson response analysis based on the assumption that SLD was governed by two repair processes gave estimated repair half-times of 0.20 [(95% confidence limits) 0.12, 0.34] and 6.6 [4.3, 10.0] h. The estimates of the short and long repair half-times were significantly different, although there was no significant difference between the results using the Poisson and logistic modes of analyses. The partition coefficient for the longer repair process was 0.5 [0.34, 0.71] indicating that about 33% of SLD-derived lethal damage is associated with the longer repair process in the case of 'complete repair' protocols. However, this proportionation is, in general, protocol dependent for incomplete repair protocols. A chi2 test on the residual deviance showed that the assumption of two repair processes for SLD gave a superior fit to the data than a single repair process at a significance level >99%. The radiation dose to the assumed target cell population depends upon their depth from the skin surface, due to the relatively short range of the electron emission from the 90Sr/90Y plaques. However, further modelling analyses have shown that the estimated repair half-times were independent of the assumed target cell distribution in the skin. This is in contrast with the alpha/beta ratio, where different (clinically significant) estimates can be obtained depending upon the assumed target cell distribution. If the target cells were at 16 micrometer depth from the surface of the skin, the estimated value for the alpha/beta ratio using the biphasic repair model would be 4.6[3.6, 5.6] Gy(Poisson analysis). However, the estimates decrease with the assumed depth (distribution) of the target cells. 相似文献
995.
M Ibe P Knolle T Beck KH Meyer zum Büschenfelde G Gerken 《Canadian Metallurgical Quarterly》1996,36(4):188-193
In Europe, transmission of HIV-1 during pregnancy occurs in 14% of children born to HIV-infected women. Risk factors for transmission are (1) virus load measured by p-24 antigenemia and HIV RNA level, (2) low CD4+ lymphocyte counts (below 600/microliter, (3) placental membrane inflammation and (4) time interval between membrane rupture and delivery. Breast feeding and vaginal delivery increase the risk of transmission of HIV infection. Antiretroviral therapy with zidovudine (Retrovir) at a dose of 500 mg/day reduces the transmission of HIV infection by two thirds. No malformation of the newborn due to zidovudine has been reported so far, but the possibility of unknown long-term adverse effects on children exposed to zidovudine must be weighed against the benefit of a considerable decrease in HIV transmission. Pregnancy is not associated with a higher rate of progression to AIDS, and HIV infection has no adverse effect on the pregnancy outcome in asymptomatic women. 相似文献
996.
A Rusek B Bassalleck A Berdoz T Bürger M Burger RE Chrien GE Diebold H En'yo H Fischer GB Franklin J Franz T Iijima K Imai J Lowe R Magahiz A Masaike CA Meyer R McCrady F Merrill S Mihara JM Nelson K Okada PH Pile B Quinn E R?ssle N Saito R Sawafta H Schmitt RA Schumacher RL Stearns R Stotzer R Sukaton R Sutter F Takeutchi DM Wolfe K Yamamoto S Yamashita S Yokkaichi V Zeps R Zybert 《Canadian Metallurgical Quarterly》1996,54(1):R15-R19
997.
998.
999.
Undoped and In-doped films of CdS were deposited at different temperatures by the chemical vapour deposition technique on glass and In-coated (30 nm) glass, respectively. Both kinds of film present a columnar microstructure, low porosity and good adherence to the substrate. The doped films have higher electron mobility values compared with the undoped ones. Shifts in the transmission spectra (Moss-Burstein effect) were observed with increased doping. Small crystals grew over the doped films during the deposition stage, mainly at the higher substrate temperatures. 相似文献
1000.
U Junghans A Koops A Westmeyer J Kappler HE Meyer HW Müller 《Canadian Metallurgical Quarterly》1995,7(11):2341-2350
Serum-free cultures of meningeal fibroblasts synthesize and release a chondroitin sulphate proteoglycan (CSPG) that markedly enhances survival but not adhesion of embryonic rat (embryonic day 15) neocortical neurons in vitro. The active molecule was purified from conditioned medium (meningeal cell-conditioned medium, MCM) in three steps by means of fast-performance liquid chromatography fractionation combined with a quantitative microphotometric bioassay: (i) preparative Q-Sepharose anion exchange chromatography under native conditions; (ii) rechromatography of biologically active Q-Sepharose fractions on a MonoQ column in the presence of 8 M urea; and (iii) final gel filtration of active MonoQ fractions on Superose 6 in the presence of 4 M guanidinium hydrochloride. Analytical sodium dodecyl sulphate-polyacrylamide gradient gel electrophoresis of active Superose 6 fractions revealed a single broad glycoprotein band with a molecular mass in the range of 220-340 kDa. Further characterization of the purified molecule with glycosaminoglycan:lyases revealed a core protein of 50 kDa and the nearly complete loss of neurotrophic activity after chondroitinase digestion, whereas heparitinase treatment changed neither electrophoretic mobility nor biological activity. Amino-terminal sequencing of the purified CSPG core protein revealed identity with the amino acid sequence of rat biglycan. Biglycan purified from bovine cartilage supported neuron survival with virtually the same activity as the CSPG purified from MCM (half-maximal activity approximate to 10(-8) M). In conclusion, we isolated a neurotrophic CSPG from meningeal cells with strong survival-enhancing activity for brain neurons that was identified as biglycan, a molecule not previously related to neural functions. 相似文献