Diagnosis of African horsesickness

African horsesickness (AHS) is a very serious, non-contagious disease of horses and other solipeds caused by an arthropod-borne orbivirus of the family Reoviridae. The epizootic nature of the disease makes rapid, accurate diagnosis of AHS absolutely essential. Currently, diagnosis of AHS is based on typical clinical signs and lesions, a history consistent with vector transmission and confirmation by laboratory detection of virus and/or anti-AHS virus antibodies. The clinicopathologic presentation of AHS, current and next generation laboratory diagnostic methods are discussed.     

Role of paired basic residues in the expression of active recombinant galactosyltransferases from the bacterial pathogen Neisseria meningitidis

The lgtB gene encoding a beta-1,4-galactosyltransferase gene and the lgtC gene encoding an alpha-1,4-galactosyltransferase from the bacterial pathogen Neisseria meningitidis were cloned into an expression vector and overexpressed in Escherichia coli. Both genes expressed very well, but problems with C-terminal proteolysis were encountered with both proteins. The lgtC protein was initially isolated from extracts of recombinant E.coli as a truncated species that retained enzymatic activity, and was subsequently shown by mass spectrometry to be 19 residues shorter than the expected protein. A specific set of engineered C-terminal deletions was constructed to investigate their effect on the expression of lgtC. As many as 28 residues could be deleted with little effect on activity, and with the concomitant improvement of the overall expression up to fivefold over the full length protein. The lgtB protein was also proteolysed in extracts of normal E.coli strains into enzymatically inactive fragments lacking 28 or 41 C-terminal residues. This degradation could be prevented by expression in an ompT protease deficient strain of E.coli. The full length lgtB protein was not stable in soluble protein extracts from all recombinant strains, however a stable enzyme preparation could be achieved with the membrane fraction from cells of the ompT deficient strain expressing lgtB. Specific deletions of lgtB were also constructed, and 15 residues could be removed without loss of enzyme activity and also with the concomitant improvement of the overall expression up to twofold over the full length protein. Longer deletions produced protein but activity could not be detected in these recombinant strains. Examination of the glycosyltransferase sequences from a wide range of bacteria showed their C-terminal segments of approximately 50 amino acids frequently contained paired basic residues. Engineering of these segments may therefore be required as a general practice to produce these enzymes for use in the large scale chemi-enzymatic synthesis of carbohydrate-based therapeutics.      

Finite element investigation of the structural behaviour of deck slabs in composite bridges

This research studies the structural behaviour of bridge deck slabs under static patch loads in steel–concrete composite bridges and investigates compressive membrane action (CMA) in concrete bridge decks slabs, which governs the structural behaviour. A non-linear 3D finite element analysis models was developed using ABAQUS 6.5 software packages. Experimental data from one-span composite bridge structures are used to validate and calibrate the proposed FEM models. A series of parametric studies is conducted. The analysis results are discussed and conclusions on the behaviour of the bridge decks are presented.     

Use of hydrocarbons as drop-in replacements for HCFC-22 in on-farm milk cooling equipment

Many refrigeration systems on New Zealand dairy farms use HCFC-22 which is being phased out by 2015. Both laboratory and on-farm trials were undertaken to investigate hydrocarbons as drop-in replacements to HCFC-22 in milk silo refrigeration systems. A mixture of propane and ethane (Care-50) reduced energy use by 6–8%, and had similar system cooling capacity relative to HCFC-22. With propane (Care-40), energy use decreased by 5% but cooling capacity was 9% lower. The retrofits were simple and low cost because no alterations to the systems other than change in refrigerant and appropriate safety labelling and documentation were made. For most farms, the outside refrigeration system location and small charge mean that hydrocarbons could meet NZ standards for safe use of refrigerants. The low retrofit cost, improved energy efficiency, low environmental impact, mineral oil compatibility, similar cooling capacity and controllable flammability risks mean that the propane–ethane mixture is an attractive replacement for HCFC-22 on NZ dairy farms.     

Extension of soil thermal conductivity models to frozen meats with low and high fat content

Thermal conductivity models of frozen soils were analyzed and compared with similar models developed for frozen foods. In total, eight thermal conductivity models and 54 model versions were tested against experimental data of 13 meat products in the temperature range from 0 to −40 °C. The model by deVries, with water+ice (wi) as the continuous phase, showed overall the best predictions. The use of wi leads generally to improved predictions in comparison to ice; water as the continuous phase is beneficial only to deVries model, mostly from −1 to −20 °C; fat is advantageous only to meats with high fat content. The results of this work suggest that the more sophisticated way of estimating the thermal conductivity for a disperse phase in the deVries model might be more appropriate than the use of basic multi-phase models (geometric mean, parallel, and series). Overall, relatively small differences in predictions were observed between the best model versions by deVries, Levy, Mascheroni, Maxwell or Gori as applied to frozen meats with low content of fat. These differences could also be generated by uncertainty in meat composition, temperature dependence of thermal conductivity of ice, measurement errors, and limitation of predictive models.     

Simulation of transient behaviour in refrigeration plant pressure vessels: mathematical models and experimental validation

Four dynamic models of different degrees of complexity were derived to represent a typical industrial refrigeration intercooler (pressure vessel). The models were validated against temperature and pressure data from two pilot plant calorimeters containing R-134a under a variety of transient operating conditions. The measured response rate was strongly influenced by sensible heat storage in the calorimeter shell and liquid refrigerant. Little difference in predictions by the four models was obtained in spite of major simplifying assumptions made to develop the less complex models. A model considering only the thermal capacity in the shell and liquid refrigerant predicted rates of temperature change within 10% of predictions by the other models, and also close to experimental data.     

A generalised mathematical modelling methodology for the design of horticultural food packages exposed to refrigerated conditions Part 2. Heat transfer modelling and testing

Sub-models required to implement the generalised simulation system, described in Part 1 for predicting in-package cooling rates of horticultural produce, are presented. These are based on heat transfer principles, but their derivation also involved application of heuristics derived by engineering judgement. Testing used two different products, eight package designs and data from four independent sources. Lack of fit between experiment and prediction was no larger than could be explained by likely data uncertainties so there is confidence that the simulation system will be accurate for a wide range of products and package designs. The object-oriented design of the simulation package allows individual sub-models to be updated without affecting any other sub-model, thereby ensuring the simulation package can remain up-to-date. In Part 3 the mass transfer sub-models are presented and test results reported.     

Simple mathematical model for predicting the transient behaviour of an ice-bank system

The time-variable performance of a Refrigerant 22 ice-bank system was simulated by a dynamic model which was derived by assuming that heat transfer was always the limiting process, and which thus ignored hydrodynamic processes. The model comprised four ordinary differential equations describing the position of the ice front, the water temperature, and the refrigerant evaporation and condensation temperatures, each of which was derived by energy balance, plus a number of algebraic equations. Measured plant performance was accurately predicted except immediately after start-up, and in circumstances in which the assumption that the dynamics of refrigerant flow did not exert any controlling influence on the overall process dynamics was inadequate (for example, when the thermostatic expansion valve operation becomes unstable). The model requires only data that should be readily available or can be easily estimated, and thus it is suitable for analyses in the design of ice bank systems to handle time-varying conditions. Simple dynamic models ignoring hydrodynamics can be adequate in circumstances where the main source of variation arises beyond the refrigeration circuit itself.