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71.
Spontaneous Ni2+ entry (leak), measured as fluorescence quench in fura-2-loaded HL-60 cells at the excitation wavelength of 360 nm, was strongly inhibited by tetrandrine (TET, 100 microM), a Ca2+ antagonist of Chinese herbal origin. Exposure of the cells for 5 min to saponins from Quillaja saponaria (QS, 30 microg/ml), surfactants well known to permeabilize the plasma membrane by complexing with cholesterol, promoted Ni2+ entry without causing fura-2 leak-out. Unexpectedly, TET caused an immediate (within 2.5 min) augmentation of QS-promoted Ni2+ entry; and a 5-min treatment with both TET and QS resulted not only in an enhanced Ni2+ entry, but also a fura-2 leak-out. Ginseng saponins (100 microg/ml) alone or together with TET did not cause such a permeabilization. Permeabilization induced by 1-3 microM digitonin, another cholesterol-complexing glycoside, could not be enhanced by TET. TET did not affect permeabilization induced by Triton X-100 (0.01%), a detergent which non-specifically disrupts the hydrophobic interaction at the plasma membrane. TET also did not enhance Ni2+ entry triggered by ionomycin (0.35 microM) or SK&F 96365 (20 microM). Further, it did not augment Ni2+ entry when the plasma membrane fluidity was modulated by changes of temperature (27-47 degrees C) or treatment with 5% ethanol. This QS-promoted Ni2+ entry could not be amplified by other lipophilic Ca2+ antagonists, such as diltiazem (100 microM) and verapamil (100 microM). The results hence indicate that TET enhanced Ni2+ entry (or permeabilization) elicited by QS treatment, but not other perturbations of the plasma membrane. We suggest that pore formation at the plasma membrane, a consequence of QS-cholesterol interaction, can be specifically enhanced by TET. Also, a comparative study of the effects of TET and its very close analogues, hernandezine and berbamine, reveals that the methoxyl group at the R2 position of TET appears to be crucial in enhancing QS-promoted Ni2+ entry. 相似文献
72.
73.
K. T. Chiang D. H. Loh P. K. Liaw E. S. Diaz 《Metallurgical and Materials Transactions A》1995,26(12):3249-3255
Flexural fatigue behavior was investigated on titanium (Ti-15V-3Cr) metal matrix composites reinforced with cross-ply, continuous
silicon carbide (SiC) fibers. The titanium composites had an eightply (0, 90, +45, -45 deg) symmetric layup. Mechanistic investigation
of the fatigue behavior is presented in Part I of this series. In Part II, theoretical modeling of the fatigue behavior was
performed using finite element techniques to predict the four stages of fatigue deflection behavior. On the basis of the mechanistic
understanding, the fiber and matrix fracture sequence was simulated from ply to ply in finite element modeling. The predicted
fatigue deflection behavior was found to be in good agreement with the experimental results. Furthermore, it has been shown
that the matrix crack initiation starts in the 90 deg ply first, which is in agreement with the experimental observation.
Under the same loading condition, the stress in the 90 deg ply of the transverse specimen is greater than that of the longitudinal
specimen. This trend explains why the longitudinal specimen has a longer fatigue life than the transverse specimen, as observed
in Part I.
This article is based on a presentation made in the symposium entitled “Creep and Fatigue in Metal Matrix Composites” at the
1994 TMS/ASM Spring meeting, held February 28–March 3, 1994, in San Francisco, California, under the auspices of the Joint
TSM/SMD/ASM-MDS Composite Materials Committee. 相似文献
74.
A novel compensator segmentation scheme for rule-based control of a linear system characterized by structured uncertainties is presented. It is assumed that the design of a segmented compensator becomes necessary because the parameter variation region is too wide to be handled by a single robust compensator. The segmentation scheme is based on the simulated annealing technique. Starting with an initial estimate of the number of segments, the offline segmentation scheme attempts to minimize it while assuring coverage of the entire parameter variation region. The information about the segment boundaries and the appropriate compensators is then utilized as the database of a rule-based switching controller. The results of simulation studies that demonstrate the performance of the proposed scheme are included 相似文献
75.
Choon Kiong Quek Ai Poh Loh 《Automatic Control, IEEE Transactions on》1997,42(4):549-553
We address a control design problem related to decoupling, namely robust decoupling. The idea of robust decoupling is defined and shown to be equivalent to robust performance of a Hadamard-weighted ℒ1 performance measure. A necessary and sufficient condition to determine if a compensator satisfies robust performance of a Hadamard-weighted ℒ1 performance measure in the presence of structured uncertainty is given. This condition can be used to determine if a compensator robustly decouples a plant. Finally, an iterative scheme for synthesizing a robust decoupling controller is discussed 相似文献
76.
The device characteristics of Er3+,Yb3+ single frequency fiber lasers are reported. A 5-cm long 1550-nm distributed feedback fiber laser with 4 mW output power is shown to have excellent specifications in terms of optical linewidth, signal-to-noise ratio (SNR), relative intensity noise, side-mode suppression and polarization purity. For higher power applications, a 1.5 cm single frequency Er3+,Yb3+ grating-based fiber laser with 60 mW output power and a net efficiency of 12% is demonstrated 相似文献
77.
Chin‐Hsiung Loh Shiuan Wan Wen‐I Liao 《The Structural Design of Tall and Special Buildings》2002,11(3):155-169
This paper summarizes the results of a study that is to evaluate the structural response attributes of near‐fault ground motion. Ground motion recordings from the Chi‐Chi earthquake are used as inputs to the structural system. An improved nonlinear hysteretic model, based on the experimental study, was used to calculate the response of the single degree‐of‐freedom inelastic system. Comparison of the results of analysis with traditional elastic–perfect plastic mode calculations was made. Discussions on the inelastic design spectrum, particularly the code‐specified base shear coefficients, using the improved nonlinear hysteretic model incorporated with the near‐fault input ground motion are made. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
78.
Ning Y. Wong Y.S. Fuh J.Y.H. Loh H.T. 《Automation Science and Engineering, IEEE Transactions on》2006,3(1):73-80
This paper discusses the effect of geometric shape on the accuracy of direct metal laser sintering (DMLS) prototypes. The percentage shrinkages due to different shapes are investigated and their empirical relationship is determined. A new speed-compensation (SC) method is proposed to reduce uneven shrinkage affected by the two-dimensional geometric shape at each layer. From case studies conducted, the optimized SC method is found to be efficient in improving the accuracy of prototypes fabricated. Note to Practitioners-This paper aims to address the problem of dimensional errors of parts built by the direct metal laser sintering (DMLS) process. Existing compensation approaches are normally based on a general relationship between the nominal dimensions and the errors after sintering. However, the effect arising from different geometric shapes is not considered. A new approach is proposed using different scan speed settings to compensate for the effect of geometric shapes to improve the dimensional accuracy of the entire part. During processing, the laser sinters along the trajectory are guided by the hatch vectors or dexel. An appropriate experimental method is used to establish the relationship for different scan speeds with the dexel length to the final accuracy. When building the part, the laser scan speed is adjusted dynamically according to the dexel length which varies with the geometric shape of the part. The case study demonstrates that the proposed method can generate correct speed settings to effectively increase the dimensional accuracy of the final part. Although this method has been developed based on the DMLS process, it is also applicable to other laser sintering processes. In future research, other process parameters, such as laser power, will be considered independently, or together with the scan speed, for possible further improvement on the dimensional accuracy. 相似文献
79.
Even though oil palm (Elaeis quineensis) stem (OPS) is highly potential as an alternative raw material in wood industry, it possesses some inferior characteristics. One of the critical weaknesses is a high degree of veneer surface roughness that resulted in high resin consumption during plywood manufacture. The objective of this study was to investigate effects of treatment with low molecular weight phenol formaldehyde (LMWPF) resin on the wettability and surface roughness of OPS veneer. OPS veneers were segregated into two categories namely outer and inner layer veneer, prior to soaking in LMWPF resin solution to obtain weight percent gain of 16–20%. The wettability of OPS veneers was assessed with contact angle measurement according to the sessile drop method. The veneer surface roughness was evaluated by determining the average roughness (Ra), mean peak-to-valley height (Rz), and maximum roughness (Rmax) using a stylus profilometer in accordance with DIN standard 4768. The results show that the effect of LMWPF resin treatment on the surface roughness of the veneers is statistically significant. The technique used in the study was able to enhance the surface properties as well as improved the physical and mechanical properties of OPS plywood. 相似文献
80.