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61.
Mutations in the human mismatch repair protein hMSH2 have been found to cosegregate with hereditary nonpolyposis colorectal cancer (HNPCC). Previous biochemical and physical studies have shown that hMSH2 forms specific mispair binding complexes with hMSH3 and hMSH6. We have further characterized these protein interactions by mapping the contact regions within the hMSH2-hMSH3 and the hMSH2-hMSH6 heterodimers. We demonstrate that there are at least two distinct interaction regions of hMSH2 with hMSH3 and hMSH2 with hMSH6. Interestingly, the interaction regions of hMSH2 with either hMSH3 or hMSH6 are identical and there is a coordinated linear orientation of these regions. We examined several missense alterations of hMSH2 found in HNPCC kindreds that are contained within the consensus interaction regions. None of these missense mutations displayed a defect in protein-protein interaction. These data support the notion that these HNPCC-associated mutations may affect some other function of the heterodimeric complexes than simply the static interaction of hMSH2 with hMSH3 or hMSH2 with hMSH6. 相似文献
62.
Growth and voluntary feed intake (VFI) in grazing temperate farmed deer species are influenced by the feeding value of the forage and the stage of the deer's seasonal cycle. Liveweight gain (LWG) of growing red deer was greater when perennial ryegrass (0.80)/white clover (0.20) pasture was grazed at 10 cm than 5 cm surface height, but venison production by one year of age was still low. Chicory and red clover were of superior feeding value for deer than perennial ryegrass-based pastures, increasing LWG of young red and hybrid (0.25 elk; 0.75 red deer) deer during summer and autumn, due to higher VFI and to higher organic matter digestibility. Relative to perennial ryegrass, chicory disintegrated faster in the rumen, with very low rumination time and faster rumen outflow rates of liquid and particulate matter. Inputs of red clover and chicory substantially increased venison carcass weights at one year of age from grazing red and hybrid stags. In indoor pen feeding studies, red deer were shown to have a seasonal cycle of digestive function, with greater rumen mean retention time (MRT) and greater rates of rumen ammonia production in summer than in winter. It was deduced that the purpose of the digestive cycle was to maintain apparent digestibility constant as VFI increased during summer. Tropical sambar deer were also shown to have seasonal cycles in growth and VFI that were of reduced amplitude compared with red deer. Peak VFI and growth occurred in autumn and minimum VFI and growth occurred in spring. Feed conversion (kg DM eaten/kg LWG) was more efficient for sambar than for red deer, due to lower VFI and lower heat production, but there was no difference between species in digestive efficiency. 相似文献
63.
M Visser DP Kiel J Langlois MT Hannan DT Felson PW Wilson TB Harris 《Canadian Metallurgical Quarterly》1998,49(5-6):745-747
Aim of the study was investigate the cross-sectional relationship between body composition and bone mineral density (BMD) in very old men and women. The study sample consisted of 504 women and 285 men, aged 72-93 yr, participating in examination 22 (1992-1993) of the Framingham Heart Study. Total body BMD, regional BMD, and soft-tissue body composition was measured by dual-energy X-ray absorptiometry. Both muscle mass and percentage body fat were positively associated with total body BMD in women. After adjustment for age, physical activity, smoking status, estrogen use, and thiazide use, BMD increased with increasing tertile of muscle mass (p = 0.007) and with increasing tertile of percentage body fat (p = 0.0001) in women. In men muscle mass, not percentage body fat, was positively associated with BMD. After adjustment for potential confounders, BMD remained associated with muscle mass only (p = 0.02). These results were similar for leg BMD and arm BMD. The study suggests that the influence of muscle and fat mass on bone mineral density is different between very old men and women. 相似文献
64.
Wang K. Bryant C. Carlson M. Elmer T. Harris A. Garcia M. Hui C.S. Leong C.K. Reynolds B. Tang R. Weber L. Wenzel J. Wilson G. Becker M. 《Micro, IEEE》1995,15(2):44-49
The MPC105 peripheral component interconnection bridge/memory controller provides a platform-specification-compliant bridge between Power PC microprocessors and the PCI bus. With it, designers can create systems using peripherals already designed for a variety of standard PC interfaces. This bridge chip also integrates a secondary cache controller and high-performance memory controller that supports DRAM or synchronous DRAM and ROM or flash ROM 相似文献
65.
OBJECTIVE: The objective of this study was to assess somatic and inherited androgen receptor gene mutations in families with only one affected individual. METHODS: Molecular genetic analysis of the androgen receptor gene in DNA derived from blood leukocytes from 30 families with single-strand conformation analysis, direct sequencing, and restriction fragment analysis was performed. RESULTS: In 22 families the mothers and all investigated grandmothers were heterozygous carriers. However, within the sisters and aunts, both heterozygous carriers and noncarriers were present. In eight families a de novo mutation was characterized. In three of these patients indication for somatic mosaicism was found. CONCLUSIONS: De novo mutations occur at a high rate within the androgen receptor gene (8 of 30 = 26.7%); a high proportion (3 of 8) arise after the zygote stage. Thus only direct analysis of the underlying mutation of the androgen receptor gene in the proband and his or her family can provide the basis for genetic counseling. 相似文献
66.
67.
Vaslow DF Wilson DG Rowe B Wilson MA Bianco JA 《IEEE transactions on medical imaging》1989,8(4):313-321
Parallel-hole collimators of varying design are discussed, using the display signal-to-noise ratio of R.F. Wagner et al. (Med. Phys., vol.6, p.83-94, 1979) to obtain contrast-detail diagrams and analytical expressions for the signal-to-noise ratio in SPECT (single-photon-emission computed tomography). It is concluded: (1) that the detectability of deeply embedded small objects (more than ~10 cm deep) may be improved only slightly by improvements in the resolution and geometric efficiency of parallel-hole collimators; (2) that a long-bore collimator outperforms a low-energy general-purpose collimator for objects smaller than ~2 cm, but only to a minor degree; and (3) that for improvement of small object detectability by SPECT, the most fruitful technological pursuit would probably involve development of focused collimators. 相似文献
68.
Future directions and research issues for ergonomics and advanced manufacturing technology (AMT) 总被引:1,自引:0,他引:1
In recent years, great progress has been made in the development of computer-based technologies that support the manufacturing industries. Those concerned with advanced manufacturing technology (AMT) are becoming increasingly aware of the fundamental importance of human organisational issues for the successful development, implementation, operation and maintenance of AMT. The discipline of industrial ergonomics and occupational occupational psychology, therefore, have a significant contribution to make in this field. This paper introduces the special issue, Ergonomics Matters in AMT, and presents a discussion of some of the future directions of AMT and the likely implications for ergonomics research. 相似文献
69.
The influence of the mobility reduction factor on the dominant third-harmonic distortion and effective transconductance in CMOS differential pair transconductors is examined. Analytical expressions are developed which are suitable for hand calculation and generate realistic estimates for distortion and transconductance. The results produced have been tested against SPICE simulations over a wide range of parameter values and show excellent agreement. The analysis highlights the importance of mobility degradation and reveals that the linearity of the source-coupled differential pair is actually improved as the mobility reduction factor increases. This surprising finding suggests that where 0.15, for example, acceptably low distortion levels (<60 dB for V
i
=1 V
pp
) should be achievable with the basic long-tailed pair and that complex linearization schemes may be unnecessary.This work is supported by a grant of the Science and Engineering Research Council. 相似文献
70.
We report some preliminary measurement of the erosion rate of plasma dumps when bombarded with 100 keV He+ ions at high power density ( 1 MW/m2). The expected erosion rates, based on measurements of He blistering that were made at lower power density ( 0.3 MW/m2), indicate a potentially serious problem for fusion reactors. Our tests use a reactorlike power density and produce He blisters at a rate that is slower than predicted by 2 to 4 orders of magnitude, depending on the temperature of the molybdenum target. 相似文献