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161.
Simultaneous assay of platelet adhesion at multiple shear rates within a single microfluidic channel
Xi Chen Lisha Zeng Zhenyu Wang Xiaoling Zhang Qiong Wang Zhong Yang Ning Hu Hongyan Luo Yanjian Liao Jun Yang 《Microfluidics and nanofluidics》2017,21(3):42
Cardiovascular diseases are currently the major causes of mortality in the world, especially in developed nations. As a predominant one, thrombosis is the platelet aggregation induced by a high shear rate. Platelet aggregation assay can clarify the occurrence mechanism of thrombosis, as well as be used as an important tool in the clinical diagnosis, personalized treatment, and screening of anticoagulants. Thus, relevant studies attracted considerable attention. As an important step in platelet aggregation, platelet adhesion and its detection also attract intensive concern. Thus, some analytical methods have been developed for platelet adhesion assay, and the impact of shear rate is one of the focuses. Compared with other devices, biosensors can give a more accurate result within a shorter time. Furthermore, some biosensors can achieve real-time analysis. However, only one or several shear rates can be tested at the same time, which may decrease the analytical efficiency. Meanwhile, in most cases, only the average platelet adhesion effect within a reactor is detected, and the impact of the distribution of shear rates is improperly neglected. In this study, a microfluidic device with a single channel is designed and fabricated for platelet adhesion assay. When the platelet-rich plasma flows through the collagen-modified sensing surface of the channel bottom, the interaction between platelets and collagen molecules on the entire surface can be simultaneously monitored by using a surface plasmon resonance imaging (SPRi) system. A gradient of the shear rate (0–546 s-1) could be formed within the channel by choosing a suitable depth-to-width ratio (1:5), so platelet adhesion at multiple shear rates could be monitored simultaneously. This method enables the measurement of the adhesion process of unlabeled platelets on the entire sensing surface, in vitro, at multiple shear rates. Such a system can obtain more accurate platelet adhesion result at a given shear rate than traditional methods. Furthermore, in an individual operation, platelet adhesion can be repeatedly tested at multiple points with an equal shear rate, so a much higher analytical efficiency can also be achieved. 相似文献
162.
Universal Access in the Information Society - Helping blind people to build cognitive maps of an environment is one of the aims of several assistive systems. In order to evaluate such assistive... 相似文献
163.
A multi-secret sharing scheme is a protocol to share m arbitrarily related secrets s1, … , sm among a set of n participants. In this paper, we propose an ideal linear multi-secret sharing scheme, based on monotone span programs, where each subset of the set of participants may have the associated secret. Our scheme can be used to meet the security requirement in practical applications, such as secure group communication and privacy preserving data mining etc. We also prove that our proposed scheme satisfies the definition of a perfect multi-secret sharing scheme. 相似文献
164.
Bo Ren Shaofan Li Jing Qian Xiaowei Zeng 《Computer Methods in Applied Mechanics and Engineering》2011,200(5-8):797-811
Shock wave induced spall fracture is a complex multiscale phenomenon, and it is a challenge to build a constitutive and computational model that can capture essential features of the spall fracture. In this work, we present a computational micro-mechanics model to simulate spall fracture by utilizing the multiscale micro-mechanics theory proposed by Wright and Ramesh [36] and a RKPM meshfree method. The focus of this work is to develop and demonstrate a simulation tool that is capable of simulations of spall fracture in engineering application. First, based on a well-known empirical formula, we relate the macroscale spall strength to the kinematics of micro void growth in a Representative Volume Element (RVE). The connection between micro void growth and overall kinematics of the RVE is made through the conservation of mass in the micro to macro transition process. Second, we develop a set of meshfree void growth algorithms that is tailored to represent kinematics of void nucleation, growth and coalescence, and these algorithms retain the conservation of mass, momentum, and energy during simulations of ductile spall fracture. Third, based on the Johnson–Cook model, we developed a meshfree computational formulation, and we have carried out simulations of the spall fracture of a Ti–6Al plate under impact loads to validate the model. From the simulation, we find that the interaction between the first two inelastic wave pulses plays an important role in the mechanism of spall fracture. The numerical results show that the proposed method can capture some features of the spall fracture, and it may be used to simulate the spall fracture in engineering applications. 相似文献
165.
Yi Zeng Ning Zhong Yan Wang Yulin Qin Zhisheng Huang Haiyan Zhou Yiyu Yao Frank van Harmelen 《Knowledge and Information Systems》2011,27(3):419-450
Under the context of large-scale scientific literatures, this paper provides a user-centric approach for refining and processing
incomplete or vague query based on cognitive- and granularity-based strategies. From the viewpoints of user interests retention
and granular information processing, we examine various strategies for user-centric unification of search and reasoning. Inspired
by the basic level for human problem-solving in cognitive science, we refine a query based on retained user interests. We
bring the multi-level, multi-perspective strategies from human problem-solving to large-scale search and reasoning. The power/exponential
law-based interests retention modeling, network statistics–based data selection, and ontology-supervised hierarchical reasoning
are developed to implement these strategies. As an illustration, we investigate some case studies based on a large-scale scientific
literature dataset, DBLP. The experimental results show that the proposed strategies are potentially effective. 相似文献
166.
Haenni的概率推理系统在与D-S理论相互转化的过程中进行了投影,从而不可避免地导致一些有价值信息的丢失。为此提出一种新的概率逻辑结果支持度的合成算法来避免信息的丢失。 相似文献
167.
应用TC35I模块和DS18B30单总线数字温度传感器模块,利用STC89C52RC微控制器和GSM移动通信网络中的短消息业务,设计一种基于TC35I的汽车空调温控系统,详细阐述了系统的基本组成和相关的软硬件方面的设计.系统具有实时性、操作简单、实用等优点. 相似文献
168.
多溴苊烯是潜在的一类有机污染物,本文采用密度泛函理论在B3LYP/6-311G**水平上,优化这类化合物的分子几何结构135个,获得它们在理想气态的一些热力学性质的数值,研究这些性质与取代的溴原子数目和位置的关系,根据各异构体的标准生成Gibbs自由能的相对大小,求出它们热力学稳定性的顺序。结果表明:大部分多溴苊烯分子具有平面的几何构型。最稳定及最不稳定异构体的△_fH~θ及△_fG~θ,都随Br原子数目增加而逐渐增加。溴原子数目相同的△_fH~θ和△_fG~θ与溴原子的取代位置有很大的关系,其相对稳定性由分子内Br…Br核排斥作用的强弱而决定。 相似文献
169.
文章着重讨论用MATLAB对离散事件系统进行仿真时的一些关键技术,如随机数发生器的生成、出入队列处理、换队处理等,并以排队系统为例进行仿真实验,以说明用MATLAB对离散事件系统仿真的方便性和有效性。 相似文献
170.
基于软构件的应用服务器框架及其组件管理器的研究 总被引:1,自引:0,他引:1
应用服务器技术和组件技术是近几年软件开发技术的主要方向之一。它对Web环境下的复杂应用如电子商务应用在可靠性、可伸缩性等方面有着非常重要的影响。论文在探讨应用服务器技术以及组件技术的基础上,通过结合CORBA开放性、跨平台、跨语言的特性以及EJB技术的企业事务逻辑,设计了一个可伸缩、健壮的应用服务器体系结构并实现了事务服务与组件管理器。 相似文献