Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy

Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation.     

多属性微断裂解释技术

四川盆地九龙山地区侏罗系珍珠冲组砾岩段气藏为构造—岩性气藏,受构造、储层、裂缝等多因素控制,并受地震资料品质和解释思路的影响,应用地震剖面、常规相干体切片不能对微断裂进行精细刻画。文中在构造导向滤波的基础上联合运用谱分解、新一代相干体切片和体曲率技术,精细刻画了微断裂的空间展布规律,说明多属性微断裂识别技术是可靠的断裂识别手段,其中体曲率属性对断层及微断裂的刻画更为清晰。野外地质考察及最新钻、测井资料的验证结果表明,珍珠冲组砾岩段微断裂十分发育,以北东、北西向为主。     

四川盆地剑阁地区多层系储层叠前描述

近期龙岗62井长兴组及剑门102井须家河组须三段获得重大突破,证实长兴组生物礁储层及须家河组碎屑岩储层均具有较大的勘探潜力。通过对研究区资料特征进行分析对比,在攻关中采用了纵横波阻抗叠前同时反演、体曲率属性、瞬时振幅属性等针对性技术,准确地刻画了生物礁台缘发育相带,预测了碎屑岩裂缝发育和油气有利聚集区,为研究区下一步井位部署提供了准确的资料基础,并形成了一套有效的技术流程。     

特殊盐岩地区钻井液技术

泌阳凹陷是南襄盆地内一个次凹陷，同一井筒钻遇了多层碱岩、芒硝和石膏矿藏。由于长期注水开采，钻井过程中井涌、井漏、盐溶解等井下复杂问题频繁发生。采用抗盐PAC聚合物钻井液和饱和碱钻井液，并利用地下高压饱和芒硝水，配成了芒硝无固相钻井液。现场应用表明，芒硝无固相钻井液抑制了盐层的缩径阻卡，再配合密闭取心技术，碱心收获率大于88．9％；机械钻速由原来的3．75～5．10m／h提高到7．62～9．60m／h，钻井周期由原来的58d下降到28d左右，钻井液成本大幅度下降。     

4H-SiC肖特基微型同位素电池(英文)

半导体同位素电池由于其寿命长、集成性优良、环境适应性强等特点成为解决MEMS能源问题的理想手段。利用4H-SiC材料的宽禁带特性,制造了4H-SiC肖特基同位素电池。对电池的耗尽层厚度以及掺杂浓度进行了优化设计,对肖特基金属进行了选择。使用4mCi/cm2的63Ni作为同位素电池的放射源对制造的同位素电池进行了测试。测试结果表明,该同位素电池可以获得31.3nW/cm2的功率密度、0.5V的开路电压、3.13×10-8A/cm2的短路电流密度和1.3%的转换效率。将电池的输出特性和硅基的平板型、3D结构电池输出特性进行了比较,证明4H-SiC肖特基同位素电池能够获得较高的功率密度。电池的性能可通过提升势垒高度、提高工艺质量、更换同位素等方式得到提高。     

利用微锥膜改善反射式液晶显示器件的视角

提出了一种可以改善反射式彩色液晶显示视角的微锥结构膜。模拟计算结果表明,当光从微锥的底部人射时,该膜起到准直作用,当光从微锥的顶部入射时,该膜起到散射作用。利用微锥膜可显著改善反射式干涉滤光膜的视角依赖性。由于微锥膜的作用不依赖了液晶盒结构,所以,可对多种结构的反射式彩色液晶显示有效。     

盘管外融冰问题的数学模型及数值分析

本文分析了盘管外融冰问题并建立其数学模型;利用该模型模拟了盘管外融冰过程,并对模拟结果进行了分析。分析表明,该模型比较合理,更加接近蓄冷槽内融冰过程的实际情况;循环水量对融冰持续时间有较大影响。所得结论可为今后蓄冷系统的优化计算分析提供理论根据。     

昌邑—大店一带活动断裂对地质灾害影响研究

为研究活动断裂对地质灾害的影响,选取华北板块鲁西地块与苏鲁造山带东界昌邑—大店断裂为研究对象,通过野外面积调查、构造剖面测量、工程地质样品分析测试、综合研究等手段,详细调查了区内活动构造的特征、地质灾害的发育特征,在此基础上讨论了活动断裂对地质灾害影响机制,认为受昌邑—大店构造的影响,地裂缝分布主要沿构造带分布,区内地...     

把握旅游发展新机遇,构筑县域旅游新框架

旅游业产业关联度高,就业空间大,资源消耗少,已成为全球经济产业中最具活力的＂朝阳产业＂和新的经济增长点。本文以安徽省十强县芜湖县县域旅游发展规划为例,提出应紧紧把握旅游发展的新机遇,加强自然生态环境保护和利用,加大旅游基础设施建设力度,打造一批旅游新产品,塑造县域旅游新亮点、新名片,积极构筑县域旅游新框架。