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41.
Analysis of simian hemorrhagic fever virus (SHFV) subgenomic RNAs, junction sequences, and 5' leader
Full-length simian hemorrhagic fever virus (SHFV) genome RNA (about 15 kb in length) and six subgenomic RNAs, ranging in size from 0.65 to 4.7 kb, were detected by Northern blot hybridization in MA104 cytoplasmic extracts with a 3' genomic antisense probe. The 5' regions of the two smallest subgenomic RNAs (RNAs 6 and 7) were cloned and sequenced. Sequence analysis indicated that these two RNAs contained a common 5' leader sequence joined to the subgenomic RNA bodies via a highly conserved junction sequence; the junction sequence of RNA 7 was 5'-TTAACC-3', while that of RNA 6 was 5'-TCAACC-3'. The complete 5' leader sequence (208 nt) was obtained from genomic RNA. The genomic 5' junction sequence is identical to that of RNA 7. Northern blot hybridization with an antisense 5' leader probe confirmed the presence of the complete leader sequence in all six species of subgenomic RNA. In its virion morphology, genome size, gene order, and replication strategy, SHFV is most similar to viruses such as equine arteritis virus, lactate dehydrogenase-elevating virus, and Lelystad virus/porcine respiratory and reproductive syndrome virus. 相似文献
42.
本文介绍了系统信息提示的一种方法,井重点讨论了用8088汇编语言实现的方法。它采用基本信息串表和索引表,对于使用频度较高的系统,可以大大节约系统内存,具存较好的实性用。 相似文献
43.
在对石油化工改扩建工程施工现场火灾危险因素识别评估的基础上,判定现场火灾危险等级、火灾类别,对灭火级别和保护距离进行验算,提出施工现场灭火器选型和配置方案。 相似文献
44.
Guo Dong Zhu Jing Xu Xue Jian Yan Jie Li Zhi Gang Zeng Miao Shen Li Zhang 《Thin solid films》2006,510(1-2):181-183
This paper introduces the Force Modulation technique to the study of crystallization process in ferroelectric vinylidene fluoride and trifluoroethylene copolymer films. Using this technique we have successfully visualized ferroelectric crystalline domains and observed that these ferroelectric domains grow out from amorphous phase, unite into strip-like structures, and finally congregate into a union. Force Modulation can weaken the influence of topography on imaging of ferroelectric domains, and reveal more details, which are difficult to be observed in topographical image. 相似文献
45.
46.
预测控制算法的统一格式及其在电加热炉的应用 总被引:4,自引:0,他引:4
本文根据常用的两类预测控制算法推出一种新型预测控制算法。并将其应用于一双输入/双输出的力学持久机电加热炉的温度控制系统上,获得了满意的结果:控温精度为±1.5℃/300℃,且适用于非最小相位系统。 相似文献
47.
The failure of acetabular cups via loosening, migration or fracture is partially related to the stress distribution existing in the acetabular region. The complexity of the investigation of these stresses demands the application of a variety of model testing techniques involving combined embedded strain gauging, finite element analysis, transmission and reflective photoelasticity and crack propagation studies. The application of these procedures are described and the difficulties of obtaining representative loadings and restraints are commented upon. 相似文献
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49.
Z. P. Zeng M. Buggy J. Griffin E. G. Little 《Journal of materials science. Materials in medicine》1992,3(4):255-261
To provide data for prosthesis design, the fatigue crack growth resistance of irradiated ultrahigh molecular mass polyethylene (UHMMPE) in saline solution at 37°C was determined from tests performed on compact tension specimens, comparable in size to the components in knee prostheses. The specimens were cyclically loaded by using a sinusoidal wave form at 1 Hz with a minimum-to-maximum load ratio of 0.1. Scanning electron microscopic fractography was used to examine the fracture surfaces. At higher stress levels, the Paris's Law was used to analyse the data, and a striation pattern with each striation corresponding to multi-cycles was observed. At lower stress levels, discontinuous fatigue crack growth was found, a phenomenon which dominated the fatigue life of the material and had not been reported previously in this material. A craze zone ahead of the crack tip was observed, which formed the discontinuous crack growth band with a length relevant to the Dugdale plastic zone length. 相似文献
50.
本文提出了一种广义最小均方(GLMS)算法,分析了GLMS算法的性能,并根据GLMS算法,导出一种自适应递归滤波(ARF)算法。在推导中,ARF算法避开了超稳定定理,从而考虑自适应系统的严格正实条件和Popov不等式是不必要的。计算机模拟表明ARF算法具有很高的收敛速度。 相似文献