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101.
Simultaneous Imaging of Endogenous Survivin mRNA and On‐Demand Drug Release in Live Cells by Using a Mesoporous Silica Nanoquencher 下载免费PDF全文
Peiyan Yuan Xin Mao Kok Chan Chong Jiaqi Fu Sijun Pan Shuizhu Wu Changmin Yu Shao Q. Yao 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(27)
The design of multifunctional drug delivery systems capable of simultaneous target detection, imaging, and therapeutics in live mammalian cells is critical for biomedical research. In this study, by using mesoporous silica nanoparticles (MSNs) chemically modified with a small‐molecule dark quencher, followed by sequential drug encapsulation, MSN capping with a dye‐labeled antisense oligonucleotide, and bioorthogonal surface modification with cell‐penetrating poly(disulfide)s, the authors have successfully developed the first mesoporous silica nanoquencher (qMSN), characterized by high drug‐loading and endocytosis‐independent cell uptake, which is able to quantitatively image endogenous survivin mRNA and release the loaded drug in a manner that depends on the survivin expression level in tumor cells. The authors further show that this novel drug delivery system may be used to minimize potential cytotoxicity encountered by many existing small‐molecule drugs in cancer therapy. 相似文献
102.
Berg Alexander Caroff Philippe Shahid Naeem Lockrey Mark N. Yuan Xiaoming Borgström Magnus T. Tan Hark Hoe Jagadish Chennupati 《Nano Research》2017,10(2):672-682
Ternary Ⅲ-Ⅴ nanowires (NWs) cover a wide range of wavelengths in the solar spectrum and would greatly benefit from being synthesized as position-controlled arrays for improved vertical yield,reprodudbility,and tunable optical absorption.Here,we report on successful selective-area epitaxy of metal-particle-free vertical InxGa1-xP NW arrays using metal-organic vapor phase epitaxy and detail their optical properties.A systematic growth study establishes the range of suitable growth parameters to obtain uniform NW growth over a large array.The optical properties of the NWs were characterized by room-temperature cathodoluminescence spectroscopy.Tunability of the emission wavelength from 870 nm to approximately 800 nm was achieved.Transmission electron microscopy and energy dispersive X-ray measurements performed on crosssection samples revealed a pure wurtzite crystal structure with very few stacking faults and a slight composition gradient along the NW growth axis. 相似文献
103.
Speckle photography technique is a versatile displacement/deformation mapping technique that can be applied to almost any material. It has its genesis in the laser speckle interferometry technique whereby the natural speckles created by illuminating an optically rough surface using a coherent laser beam are used as displacement gauging elements. It evolves into the white light speckle photography technique whereby a random pattern of any type is used as a displacement measurement transducer. With the advent of digital cameras and ubiquitous usage of computers, the digital version of the technique is developed. Up to now, the technique is essentially limited to 2D applications. Recently, we extended the technique into the 3D domain by using the volumetric imaging capabilities of CT or MRI. In this paper, we apply this technique to measuring the internal deformation of rocks. It so happens that certain rocks have natural density variation at various places such that when imaged by CT these impurities can be treated as 3D speckles. The elements of volumetric speckle photography technique are as follows. A reference volumetric image of the rock is recorded by a micro-CT scan and stored as a reference. Under load, the deformed CT image of the rock is also recorded. Both volume images are divided into subsets of certain voxel arrays. Each corresponding pair of the subsets is “compared” via a two-step 3D Fourier transform analysis. The result is a 3D map of displacement vectors representing the collective displacement experienced by all the speckles within the subset of voxels. The strain distribution of the entire rock specimen can then be calculated using appropriate displacement strain relations. The application of this technique to strain mapping of red sandstone and argillite rocks is presented. 相似文献
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硅酸根分析仪是利用朗伯-比尔定律原理,对水中硅酸根含量进行测量的分析仪器。该仪器的主要计量性能有:示值误差、重复性、稳定性等。现对硅酸根分析仪测量结果(即:浓度测量值的示值误差)进行不确定度评定如下,供大家参考和探讨。 相似文献
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结合工作实际,阐述了在长期检测工作中发现一些影响其计量准确性的因素,它对动态公路车辆自动衡器单轴称量示值误差不确定度的影响。 相似文献