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Rakesh V. Vohra 《IIE Transactions》1987,19(3):296-299
The (5, 7) cyclic staffing problem is the problem of finding the least number of workers assigned to a 7 day cyclic schedule, so that sufficient workers are present during day i to meet requirement bi and each person works a shift of 5 consecutive days and is idle for the other 2. In this paper we derive an expression for the minimal workforce size in the problem in terms of the bi's. This result is interesting because it shows the extra number of workers needed by insisting that each person's idle days are consecutive. 相似文献
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RF Epand JC Macosko CJ Russell YK Shin RM Epand 《Canadian Metallurgical Quarterly》1999,286(2):489-503
To better understand the roles of different regions of influenza hemagglutinin in membrane fusion, we have studied the fusion properties of large unilamellar vesicles in the presence of constructs comprising the 127 amino acid ectodomain of the HA2 fragment (FHA2) as well as mutated forms of FHA2 containing single amino acid substitutions, the 95 amino acid truncated form of FHA2 lacking the N-terminal fusion peptide (SHA2), the 20 amino acid N-terminal fusion peptide and the ten amino acid peptide corresponding to the kinked loop region of FHA2. The 100 nm liposomes were made from dioleoylphosphatidylethanolamine, dioleoylphosphatidylcholine and cholesterol in equimolar ratio. At pH 5 a high rate of lipid mixing was observed with FHA2 present, even at very low molar concentrations, whereas much lower rates were observed using the shorter constructs: SHA2, the fusion peptide, and the loop peptide. Concentrations of FHA2 which promoted extensive lipid mixing also induced leakage of aqueous contents. Marked effects of FHA2 were also observed with liposomes of egg phosphatidylcholine. All of the changes observed with the liposomes were highly pH-dependent, with only negligible changes occurring at pH 7. The results demonstrate the potent action of FHA2 in promoting lipid mixing and demonstrate the contribution of other regions of the ectodomain of FHA2, in addition to the fusion peptide, to the mechanism of acceleration of membrane fusion. The results also indicate that the pH dependence of fusion is not due solely to changes in the interactions between the HA1 and HA2 subunits. Thus, the "spring loaded energy" is not required to bring about the apposition of the two membranes, considering that FHA2 is already in its thermostable conformation. The acidic amino acid residues in the kinked loop region appear to play a particularly important role in the pH-dependent fusion process as demonstrated by the marked loss of lipid mixing activity of mutant forms of FHA2. 相似文献
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Cigarette smoking, environmental chemicals and ionizing radiations are the three factors known to cause cancer in human beings. The relative importance of each of these is being constantly evaluated. There is an urgent need to monitor the environmental carcinogens on a large scale to assess the role of environmental chemicals in the incidence of cancer in human populations. Polycyclic aromatic hydrocarbons (PAH) are released into the atmosphere as a result of fossil fuel combustion and some of the PAH (e.g. benzo(a)pyrene) are recognised carcinogens. Measurement of benzo(a)pyrene in urban, suburban and rural regions of Bombay is carried out in order to evaluate the possible correlation with lung cancer incidence among different population groups. The variations in the concentration at the three sampling locations are discussed. The wide differences in the concentration at different locations seem to be very suitable for epidemiological investigations. 相似文献
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Translation initiation of hepatitis C virus (HCV) RNA occurs by internal entry of a ribosome into the 5' nontranslated region in a cap-independent manner. The HCV RNA sequence from about nucleotide 40 up to the N terminus of the coding sequence of the core protein is required for efficient internal initiation of translation, though the precise border of the HCV internal ribosomal entry site (IRES) has yet to be determined. Several cellular proteins have been proposed to direct HCV IRES-dependent translation by binding to the HCV IRES. Here we report on a novel cellular protein that specifically interacts with the 3' border of the HCV IRES in the core-coding sequence. This protein with an apparent molecular mass of 68 kDa turned out to be heterogeneous nuclear ribonucleoprotein L (hnRNP L). The binding of hnRNP L to the HCV IRES correlates with the translational efficiencies of corresponding mRNAs. This finding suggests that hnRNP L may play an important role in the translation of HCV mRNA through the IRES element. 相似文献
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Pablo C. Caracciolo Fabián Buffa Vinoy Thomas Yogesh K. Vohra Gustavo A. Abraham 《应用聚合物科学杂志》2011,121(6):3292-3299
The development of elastomeric, bioresorbable, and biocompatible segmented polyurethanes (SPUs) for use in tissue‐engineering applications has attracted considerable interest in recent years. In this work, nonporous films and microfiber/nanofiber scaffolds, which were prepared from two different poly(?‐caprolactone)‐based SPUs previously synthesized from 1,6‐hexamethylene diisocyanate and novel chain extenders containing urea groups or an aromatic amino acid derivative, were studied. Their thermal properties were influenced by both the different chemical structures of the hard segments and the processing conditions. The mechanical properties of the scaffolds (the elastic modulus, ultimate strain, and tensile stress) were adequate for engineered soft‐tissue constructs (e.g., myocardial tissue). The film samples displayed a low swelling degree (<2 wt %) in a phosphate‐buffered solution at 37°C. The introduction of the amino acid derivative chain extender with hydrolyzable ester bonds contributed to greater degradation. The fibrous scaffolds exhibited higher hydrolytic stability than the films after short assay times because of their more crystalline structures and higher degrees of association by hydrogen bonding, but they also experienced higher mass losses under accelerated conditions (70°C). This suggested that the degradation rate was not constant but depended on the degradation time and the processing technique. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献