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951.
Antioxidant Activity of Oxygen Evolving Enhancer Protein 1 Purified from Capsosiphon fulvescens 下载免费PDF全文
Eun‐Young Kim Youn Hee Choi Jung Im Lee In‐Hye Kim Taek‐Jeong Nam 《Journal of food science》2015,80(6):H1412-H1417
This study was conducted to determine the antioxidant activity of a protein purified from Capsosiphon fulvescens. The purification steps included sodium acetate (pH 6) extraction and diethylaminoethyl‐cellulose, reversed phase Shodex C4P‐50 column chromatography. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis indicated that the molecular weight of the purified protein was 33 kDa. The N‐terminus and partial peptide amino acid sequence of this protein was identical to the sequence of oxygen evolving enhancer (OEE) 1 protein. The antioxidant activity of the OEE 1 was determined in vitro using a scavenging test with 4 types of reactive oxygen species (ROS), including the 2,2‐diphenyl‐1‐picrylhydrazyl radical, hydroxyl radical, superoxide anion, and hydrogen peroxide (H2O2). OEE 1 had higher H2O2 scavenging activity, which proved to be the result of enzymatic antioxidants rather than nonenzymatic antioxidants. In addition, OEE 1 showed less H2O2‐mediated ROS formation in HepG2 cells. In conclusion, this study demonstrates that OEE 1 purified from C. fulvescens is an excellent antioxidant. 相似文献
952.
This paper proposes a simple and fast method to identify the normal absorptance of various surfaces submitted to a radiation source, using inverse techniques. The method consists of imposing during a lap of a few seconds a radiative flux on the front face of a sample whose absorptance is to be identified. The time-dependent temperature on the rear face is measured, and the procedure of inversion is implemented to give a time function of absorbed flux. Only one time–temperature function is measured using a current type K thermocouple. The normal absorptance of the front face is obtained by comparing the time heat flux function of the source and the identified absorbed heat flux function. This method can be quickly and efficiently adopted for many practical applications without the need to use optical devices, which give accurate measurement but at substantial cost. The inverse technique using a conjugate gradient method of minimization with adjoint problem is implemented to estimate the absorbed heat flux. In order to achieve good values of radiative absorptances, reliable knowledge of thermal diffusivities and adequately manufactured samples are required. 相似文献
953.
An empirical correlation for two-phase frictional performance in small diameter tubes 总被引:1,自引:0,他引:1
Ing Youn ChenKai-Shing Yang Chi-Chung Wang 《International Journal of Heat and Mass Transfer》2002,45(17):3667-3671
Experimental two-phase pressure drop data in small diameter tubes (D<10 mm) have been collected and updated from the literature which contain eight refrigerant and three air-water datasets. Comparisons between the data and the predictions indicate that the Chisholm correlation fails to predict the data. The Friedel correlation and Souza and Pimenta's correlation give fair predictions for the refrigerant data, but fail to predict the air-water data due to the surface tension effect. The homogeneous model shows a better predictive ability (a mean deviation of 34.7%) than the other empirical correlations. In this regard, an empirical correlation based on the homogeneous model was developed. By introducing the Bond number and Weber number to the modified correlation, the new correlation gives a mean deviation of 19.1% based on 1484 data points. 相似文献
954.
Jeong Gil Seo Min Hye Youn Sunyoung Park Jin Suk Chung In Kyu Song 《International Journal of Hydrogen Energy》2009,34(9):3755-3763
Al2O3–ZrO2 (AZ) xerogel supports prepared by a sol-gel method were calcined at various temperatures. Ni/Al2O3–ZrO2 (Ni/AZ) catalysts were then prepared by an impregnation method for use in hydrogen production by steam reforming of liquefied natural gas (LNG). The effect of calcination temperature of AZ supports on the catalytic performance of Ni/AZ catalysts in the steam reforming of LNG was investigated. Crystalline phase of AZ supports was transformed in the sequence of amorphous γ-Al2O3 and amorphous ZrO2 → θ-Al2O3 and tetragonal ZrO2 → (θ + α)-Al2O3 and (tetragonal + monoclinic) ZrO2 → α-Al2O3 and (tetragonal + monoclinic) ZrO2 with increasing calcination temperature from 700 to 1300 °C. Nickel oxide species were strongly bound to γ-Al2O3 and θ-Al2O3 in the Ni/AZ catalysts through the formation of solid solution. In the steam reforming of LNG, both LNG conversion and hydrogen composition in dry gas showed volcano-shaped curves with respect to calcination temperature of AZ supports. Nickel surface area of Ni/AZ catalysts was well correlated with catalytic performance of the catalysts. Among the catalysts tested, Ni/AZ1000 (nickel catalyst supported on AZ support that had been calcined at 1000 °C) with the highest nickel surface area showed the best catalytic performance. Well-developed and pure tetragonal phase of ZrO2 in the AZ1000 support played an important role in the adsorption of steam and the subsequent spillover of steam from the support to the active nickel. 相似文献
955.
Control of aerosol contaminants in indoor air: combining the particle concentration reduction with microbial inactivation 总被引:1,自引:0,他引:1
Grinshpun SA Adhikari A Honda T Kim KY Toivola M Rao KS Reponen T 《Environmental science & technology》2007,41(2):606-612
An indoor air purification technique, which combines unipolar ion emission and photocatalytic oxidation (promoted by a specially designed RCI cell), was investigated in two test chambers, 2.75 m3 and 24.3 m3, using nonbiological and biological challenge aerosols. The reduction in particle concentration was measured size selectively in real-time, and the Air Cleaning Factor and the Clean Air Delivery Rate (CADR) were determined. While testing with virions and bacteria, bioaerosol samples were collected and analyzed, and the microorganism survival rate was determined as a function of exposure time. We observed that the aerosol concentration decreased approximately 10 to approximately 100 times more rapidly when the purifier operated as compared to the natural decay. The data suggest that the tested portable unit operating in approximately 25 m3 non-ventilated room is capable to provide CADR-values more than twice as great than the conventional closed-loop HVAC system with a rating 8 filter. The particle removal occurred due to unipolar ion emission, while the inactivation of viable airborne microorganisms was associated with photocatalytic oxidation. Approximately 90% of initially viable MS2 viruses were inactivated resulting from 10 to 60 min exposure to the photocatalytic oxidation. Approximately 75% of viable B. subtilis spores were inactivated in 10 min, and about 90% or greater after 30 min. The biological and chemical mechanisms that led to the inactivation of stress-resistant airborne viruses and bacterial spores were reviewed. 相似文献
956.
Yoichi Okawa Chongho Youn Kenji Kawashima Toshiharu Kagawa 《International Journal of Hydrogen Energy》2012
The thesis of this study is to investigate that the measurement accuracy of the isothermal discharge method for hydrogen gas with an isothermal tank which is designed for measuring the flow rate characteristics of pneumatic components. Compressed hydrogen in an isothermal tank, which is combined three types of orifice, is discharged from 700 kPa (abs) to atmospheric pressure. The average temperature in the tank during discharge is measured experimentally. In consequence, when the maximum discharge rate is 37 kPa/s during discharge hydrogen, the measurement error is less than 3% in whole discharge time. The temperature response phenomenon in hydrogen is discussed qualitatively in the view point of the internal energy change. The internal energy change immediately after the discharge started was negative because the release enthalpy was larger than the quantity of heat obtained from the stuffing material. After a certain period of time elapsed, the enthalpy change became equal to the heat exchange between the internal hydrogen and the stuffing material. 相似文献
957.
958.
The current study investigates the oxidative stability of soybean oil packaged with an oxygen scavenging film prepared by pyrogallol coating with concentrations of 5, 10, and 20% at 5, 23, and 60 °C and 95 ± 2% RH respectively. The oil stability was evaluated in terms of peroxide, thiobarbituric acid, and p-anisidine then compared with oil packed without the oxygen scavenging film. The results showed that the LDPE/PG 10 and 20% were efficient in the stabilization of soybean oil, even at high temperature. Peroxide, Thiobarbituric acid, and p-anisidine values, the oil samples packed with LDPE/PG films delayed the oil oxidation. The synergetic effect of LDPE/PG films, which can scavenge oxygen from the packaged product thereby slowing the oxidation of fats, was established in the study. The present study confirmed that active packaging could be introduced as a worthy replacement for direct addition of artificial antioxidants to the soybean oil. 相似文献
959.
960.
In‐Hah Kim Hanna Lee Jung Eun Kim Kyung Bin Song Youn Suk Lee Dae Sung Chung Sea C. Min 《Journal of food science》2013,78(10):E1551-E1559
Nanoemulsions containing lemongrass oil (LO) were developed for coating plums and the effects of the nanoemulsion coatings on the microbial safety and physicochemical storage qualities of plums during storage at 4 and 25 °C were investigated. The emulsions used for coating were produced by mixing a carnauba wax‐based solution (18%, w/w) with LO at various concentrations (0.5% to 4.0%, w/w) using dynamic high pressure processing at 172 MPa. The coatings were evaluated for their ability to inhibit the growth of Salmonella Typhimurium and Escherichia coli O157:H7 and their ability to preserve various physicochemical qualities of plums. Uniform and continuous coatings on plums, formed with stable emulsions, initially inhibited S. Typhimurium and E. coli O157:H7 by 0.2 to 2.8 and 0.8 to 2.7 log CFU/g, respectively, depending on the concentration of LO and the sequence of coating. The coatings did not significantly alter the flavor, fracturability, or glossiness of the plums. The antimicrobial effects of the coatings against S. Typhimurium and E. coli O157:H7 were demonstrated during storage at 4 and 25 °C. The coatings reduced weight loss and ethylene production by approximately 2 to 3 and 1.4 to 4.0 fold, respectively, and also retarded the changes in lightness and the concentration of phenolic compounds in plums during storage. The firmness of coated plums was generally higher than uncoated plums when stored at 4 °C and plum respiration rates were reduced during storage. Coatings containing nanoemulsions of LO have the potential to inhibit Salmonella and E. coli O157:H7 contamination of plums and may extend plum shelf life. 相似文献