Features of noradrenaline stimulation of rat liver mitochondria respiration by ADP and calcium ions

A single administration of a physiological dose of noradrenaline to animals. in contrast to adrenaline, stimulates the respiration of mitochondria not only under oxidation of FAD-dependent Krebbs cycle substrate of the succinase but also HAD-dependent substrate of alpha-ketoglutarate. In the both cases the phosphorylation rate increases, since the action of noradrenaline, separating the respiration and oxidative phosphorylation, was not found. Noradrenaline increases the capacity of mitochondria to more actively absorb calcium ions under oxidation of succinate than under that of alpha-ketoglutarate.     

白浪河大桥钢桁架结构可行性分析研究

采用Midas2013软件对潍坊白浪河大桥主桥钢桁架结构进行总体分析研究,假定钢桁架全部单元采用梁单元结构形式,所有杆件之间用固结形式模拟,材料在线弹性范围内工作,利用小变形、叠加原理分析研究,研究结果表明:该桁架桥结构稳定系数为30,具有较高的安全可靠性。     

广东电网调度操作实时信息展示系统研究∗

设计了调度实时操作信息的航班式全景展示和调度实时操作进度的物流式展示的方法并开发了系统软件.通过对调度实时运行信息的智能、动态更新,调度对象对其各工作流程节点的处理进程实现"京东购物"式的体验.     

Pharmacologically induced elements of the hunting and feeding behavior in the pteropod mollusk Clione limacina. II. Effects of physostigmine

1. A contact of the pteropod mollusk Clione limacina with its prey (small pteropod mollusk Limacina helicina) evokes a complex pattern of hunting and feeding behavior: protraction of tentacles to seize the prey, activation of buccal apparatus to swallow the prey, activation of locomotor system (speeding up of wing beating), reversal of reaction to tactile stimulation of the head, loss of normal (vertical) orientation in space, and swimming in circles. After injection of physostigmine (PhS), the acetylcholinesterase inhibitor, into the hemocoel of intact Clione, all these manifestations of the hunting and feeding behavior could be evoked by tactile stimulation of the head, or they arose spontaneously. 2. In the preparation of the isolated CNS, the effect of PhS on the neural networks controlling different aspects of the hunting and feeding behavior was studied by recording from neurons monitoring activity of different networks (< or = 4 neurons simultaneously). Tactile stimulation of the head was mimicked by a short-term electrical stimulation of the corresponding nerve. Before PhS application, the nerve stimulation evoked elements of the defense reaction, i.e., long-lasting inhibition of all main motor control systems: the locomotor network in the pedal ganglia, the tentacle control network in the cerebral ganglia, and the network controlling radula and hook movements in the buccal ganglia. However, after PhS application, the same stimulus evoked a long-lasting bout of excitation in all the three networks accompanied by activation of the heart-exciting neuron as well as by a modification of the activity of statocyst receptor cells controlling Clione's spatial orientation (the "fictive hunting bout"). Similar hunting bouts could arise spontaneously. 3. Injection of acetylcholine (ACh) into the hemocoel of intact Clione was less effective than injection of PhS. After ACh injection, reversal of reaction to head stimulation was observed in < or = 20% of the experiments (the percentage of positive results was higher if ACh was injected into the head just over the CNS). Bath application of ACh to the isolated CNS did not produce the hunting bouts. However, a short-term local application of ACh to the cerebral ganglia in the isolated CNS resulted in activation of the main motor systems controlling locomotion, protraction of tentacles, and movements of buccal mass. 4. During spontaneous PhS-induced bouts, excitation of different networks involved in hunting behavior was sometimes not quite synchronous. Different networks could be excited in variable order over a period of up to several seconds.(ABSTRACT TRUNCATED AT 400 WORDS)     

复合混凝剂处理垃圾填埋场渗滤液的研究

针对垃圾渗滤液的高浓度、难降解、具有生物毒性的特点,采用混凝沉降法对其进行处理,以处理出水的CODcr为处理程度的表征指标。通过正交实验,确定最佳复合混凝剂的试剂用量,比较不同反应条件对CODcr去除率的影响,优化操作条件。实验结果表明,最佳复合混凝剂:1 250 mg/LPAC+1 500 mg/LPFS+50 mg/L PAM;最佳条件:pH=7.0,搅拌时间为15 min,沉降时间为20 min;CODcr去除率达到66.9%。     

混合脂肪醇磷酸酯渗透剂的合成与性能研究

以脂肪醇、环氧乙烷、五氧化二磷为原料,合成了一系列脂肪醇聚氧乙烯醚磷酸酯,筛选出了理想的渗透产品,并通过多组实验数据考查不同磷酸酯的耐碱性和渗透性。检测结果表明：在强烈搅拌下,分批投入五氧化二磷,原料配比为n（脂肪醇）：n（P2O5）=2：1,正辛醇与异辛醇的混合比为1：5时,所得产物有最佳耐碱性和渗透性。     

通风条件对建筑竖井烟气运动的影响模拟

选择典型的竖井建筑结构形式,重点研究不同风速下烟气从燃烧室向竖井中迁移的规律。结果表明,在燃烧室内,风速大于0.5 m/s时CO体积分数和温度变化均非常小;风速小于0.5 m/s时,CO体积分数和温度骤降,形成明显的梯度差;在竖井中,CO浓度先上升后下降,温度逐渐下降,风速对CO的影响和燃烧室内的相同。建议该模型的最佳机械排烟风速为0.5 m/s,可以保证安全,同时实现最佳的经济效益。     

北京谱仪Ⅲ电磁量能器晶体尺寸的测量

对北京谱仪电磁量能器中晶体的分布、CaI晶体尺寸的测量所用的仪器和方法进行了分析,最后对所有晶体的尺寸进行了统计.     

Distinctive structure of the human GSTM3 gene-inverted orientation relative to the mu class glutathione transferase gene cluster

The goals of this study were 1) to compare the effects of transforming growth factor-beta (TGF-beta) and parathyroid hormone-related protein (PTHrP) on mouse blastocyst attachment and outgrowth in vitro, 2) to determine whether TGF-beta acts through a mechanism involving PTHrP, 3) to examine effects of PTHrP on preimplantation mouse embryo development, and 4) to determine the pattern of expression of PTHrP protein in the uterus of the mouse during early gestation. In the first set of experiments, hatched blastocysts were placed in fibronectin-coated wells. Cultures were treated with PTHrP or TGF-beta1 and assessed at 24, 48, and 72 h for attachment and surface area of blastocyst outgrowth. Results showed that both PTHrP and TGF-beta1 increased blastocyst outgrowth significantly. A PTHrP-neutralizing antibody blocked the stimulatory effect of both PTHrP and TGF-beta1, suggesting that TGF-beta1 acts to increase endogenous production of PTHrP by the blastocyst. Immunoassay of conditioned medium from blastocysts treated with either TGF-beta1 or PTHrP 1-34 confirmed a 3- to 4-fold increase in levels of PTHrP 1-141. In the second series of experiments, pronuclear zygotes were cultured in various concentrations of PTHrP for 96 h. Blastocysts then were subjected to differential fluorescent staining of inner cell mass and trophectoderm cells. Treatment of mouse embryos with the various concentrations of PTHrP altered neither the number developing to the blastocyst stage nor the number of inner cell mass or trophectoderm cells in the resulting blastocysts. In the third experiment, pregnant mice were killed at Days 3, 4, 5, 6, and 7 of gestation, and uterine horns were processed for immunohistochemistry. Uterine sections were stained with antibodies to PTHrP, desmin, and laminin. On Days 3, 4, and 5, uterine luminal and glandular epithelial cells stained intensely for PTHrP, while stromal cells were negative. By Days 6 and 7, decidualized stromal cells stained positively for PTHrP, desmin, and laminin. These results support the hypothesis that TGF-beta and PTHrP play an important role in the process of implantation.     

Separation from related compounds and assay of calcipotriol by high-performance liquid chromatography

In this paper, two methods are presented. One involves the separation of calcipotriol, a new synthetic analogue, from two related compounds, specifically cholecalciferol (Vitamin D3) and calcitriol (1,25-dihydroxyvitamin D3). The other involves the isolation and assay of calcipotriol from a topical ointment. The study was performed with reversed-phase high-performance liquid chromatography using an RP18 column and ultraviolet detection. Applying the method of Snyder, a mobile phase mixture containing methanol-acetonitrile-water (67:23:10, v/v) was found which achieved a total separation within 18 min. A mobile phase of methanol-water (80:20, v/v) attained a slower elution of calcipotriol. For isolation and assay of calcipotriol from an ointment (Daivonex), dissolution in chloroform gave the highest recovery (> 98%). The isolation and assay process can be performed within 2 h.