Microscale epitope mapping by affinity capillary electrophoresis-mass spectrometry

Using beta-endorphin as a model system, a new microscale solution-based approach for linear epitope mapping based on affinity capillary electrophoresis-mass spectrometry (ACE-MS) is demonstrated. Tryptic peptides are separated in a neutral coated capillary and monitored by ultraviolet absorbance and electrospray mass spectrometry. Then, following injection of the peptide digest mixture, anti-beta-endorphin antibody is injected. The peptide, which binds to the antibody, is captured and disappears from its migration time. Following this subtraction-screening procedure, the binding of the individually synthesized or isolated immunoreactive peptide is examined by the ACE-MS procedure to confirm that the epitope resides on the peptide. A series of truncated peptides can then be made and the precise epitope determined by ACE-MS. The method utilizes low femtomole amounts of antibody and peptide digest per run and is rapid and easily automatable.     

Simultaneous phenytoin and warfarin toxicity on chronic concomitant therapy

Pneumoperitoneum in diagnostic or therapeutic laparoscopy can be achieved by an open or a closed technique. The authors, on the basis of their experience, compare both techniques, concluding that open method is safer, because of the lack of major complications observed in the closed method and with only few minor complications.     

樱桃红色素光和热稳定性的研究

实验主要研究了樱桃(毛樱桃、草原樱桃)果实的光和热的稳定性,结果表明:两种樱桃红色素热稳定性较好,60℃以下加热对色素无影响,超过60℃色素容易被氧化,光照对樱桃色素稳定性有严重不良影响。