Inversin, a novel gene in the vertebrate left-right axis pathway, is partially deleted in the inv mouse

A DNA fragment containing the recA gene of Gluconobacter oxydans was isolated and further characterized for its nucleotide sequence and ability to functionally complement various recA mutations. When expressed in an Escherichia coli recA host, the G. oxydans recA protein could efficiently function in homologous recombination and DNA damage repair. The recA gene's nucleotide sequence analysis revealed a protein of 344 amino acids with a molecular mass of 38 kDa. We observed an E. coli-like LexA repressor-binding site in the G. oxydans recA gene promoter region, suggesting that a LexA-like mediated response system may exist in G. oxydans. The expression of G. oxydans recA in E. coli RR1, a recA+ strain, surprisingly caused a remarkable reduction of the host wild-type recA gene function, whereas the expression of both Serratia marcescens recA and Pseudomonas aeruginosa recA gene caused only a slight inhibitory effect on function of the host wild-type recA gene product. Compared with the E. coli RecA protein, the identity of the amino acid sequence of G. oxydans RecA protein is much lower than those RecA proteins of both S. marcescens and Pseudomonas aeruginosa. This result suggests that the expression of another wild-type RecA could interfere with host wild-type recA gene's function, and the extent of such an interference is possibly correlated to the identity of the amino acid sequence between the two classes of RecA protein.     

层次式级联多网络模型及其性能

本文提出了一种层次式级联多网络结构，论述了其训练算法，并验证了该结构的收敛性。这种结构在处理高阶非线性分布数据的分类问题方面具有优良的性能，用北京谱仪事例过滤进行性能测试结果表明，其训练和泛化性能都优于单个ＢＰ网络。     

三峡工程左厂房坝导墙抗震计算分析

 用反应谱理论对三峡左厂房坝导墙3种典型设计断面型式进行了抗震计算分析比较，得出了其自振特性及地震动位移、动应力响应。对选定的设计断面型式Ⅲ进行了抗拉应力强度安全性评价。     

单色光石英退偏器的退偏机理及性能研究

研究了单色光石英退偏器的退偏机理；定义了退偏度作为衡量退偏器性能的物理量。从实验上在４４０～９２０ｎｍ的光谱范围测试了器件的退偏度，结果表明：只要器件的结构角适当，或者适当调节入射光束的截面大小，便可对某一单色线偏振光实现很好的退偏。     

地方时与区时在电离层研究中的差异

本文指出混淆地时与区时两个时间概念在电离层研究中带来的差异的严重性，建议使用地方与区时这两个名词，而抛弃“本地时”和“当地时”等容易引起混淆的名词。     

利用调和映射的复杂网格曲面螺旋刀轨生成算法

针对截平面法规划的复杂网格曲面刀轨的加工效率不高问题,提出一种复杂网格曲面螺旋刀轨生成算法.首先采用调和映射的方法对网格曲面进行参数化,然后根据残留高度确定参数网格中的参数环,在相邻参数环的参数点之间进行“分组匹配”,并依次计算初始和精确的对角参数螺旋线;在此基础上采用“区域划分”的方法快速生成了无干涉的网格曲面螺旋刀轨.对于复杂网格曲面的实验结果表明,文中的螺旋刀轨能够有效地提高截平面法刀轨的加工效率,且能够保证较好的加工质量.     

基于视频的现实人物运动创作

提出一种根据用户指定的人体运动和观察视角生成真实感视频的方法.首先采集演员进行少数基本运动时的多视角视频数据库,并使用无标记运动捕捉的方法获得任意时刻人体对应的骨骼和3D模型.其次,用户对人体骨架指定运动并设定视角,以此定义目标视频.实验结果验证了文中方法能够利用有限的数据库合成演员在用户指定运动和视角下的真实感视频.     

基于GIS的黑龙江省多年平均降水量空间插值分析比较

以黑龙江省分辨率为90m的数字高程模型(DEM)的数据为基础,结合ArcGIS软件,分析黑龙江省83个气象站点1971—2018年的降水资料,采用克里金法(Kriging)、反距离权重法(IDW)、径向基函数法(RBF)、全局多项式插值法(GPI)和趋势面法(TS)五种方法进行空间插值模拟.结果表明,黑龙江省年平均降水量东多西少,具有自边缘到中心逐渐增加的特征.西南部和西北部(漠河和北极村)的降水量大多在500mm以下,部分中部地区的降水量超过700mm.总体年平均降水量在400mm—700mm之间,大部分地区属于半湿润区.在该研究区域中,通过克里金指数模型插值方法获得结果的准确性优于其他插值方法.     

U形预应力高强度混凝土板桩在大沙河综合整治工程中的应用

U形板桩是一种新型的预应力钢筋混凝土预制构件,主要应用于河道、湖泊护岸治理、港口码头、城市基坑支护等工程.通过大沙河治理工程实例,分别对U形板桩护岸、灌注桩护岸、悬臂式挡墙护岸和生态砖护岸进行了分析对比,优选出技术可靠、安全稳定、经济合理的U形板桩护岸结构作为工程的处理方案.此类应用可以增强河岸美观效果并节约投资,在加快施工速度的同时减少对周边环境影响,可为类似工程提供参考.     

Maximizing learning without sacrificing the fun: Stealth assessment,adaptivity and learning supports in educational games

In this study, we investigated the validity of a stealth assessment of physics understanding in an educational game, as well as the effectiveness of different game-level delivery methods and various in-game supports on learning. Using a game called Physics Playground, we randomly assigned 263 ninth- to eleventh-grade students into four groups: adaptive, linear, free choice and no-treatment control. Each condition had access to the same in-game learning supports during gameplay. Results showed that: (a) the stealth assessment estimates of physics understanding were valid—significantly correlating with the external physics test scores; (b) there was no significant effect of game-level delivery method on students' learning; and (c) physics animations were the most effective (among eight supports tested) in predicting both learning outcome and in-game performance (e.g. number of game levels solved). We included student enjoyment, gender and ethnicity in our analyses as moderators to further investigate the research questions.