A simple and cost-effective method for fabrication of integrated electronic-microfluidic devices using a laser-patterned PDMS layer

We report a simple and cost-effective method for fabricating integrated electronic-microfluidic devices with multilayer configurations. A CO₂ laser plotter was employed to directly write patterns on a transferred polydimethylsiloxane (PDMS) layer, which served as both a bonding and a working layer. The integration of electronics in microfluidic devices was achieved by an alignment bonding of top and bottom electrode-patterned substrates fabricated with conventional lithography, sputtering and lift-off techniques. Processes of the developed fabrication method were illustrated. Major issues associated with this method as PDMS surface treatment and characterization, thickness-control of the transferred PDMS layer, and laser parameters optimization were discussed, along with the examination and testing of bonding with two representative materials (glass and silicon). The capability of this method was further demonstrated by fabricating a microfluidic chip with sputter-coated electrodes on the top and bottom substrates. The device functioning as a microparticle focusing and trapping chip was experimentally verified. It is confirmed that the proposed method has many advantages, including simple and fast fabrication process, low cost, easy integration of electronics, strong bonding strength, chemical and biological compatibility, etc.     

Electric field-induced concentration and capture of DNA onto microtips

Simple, high-yield concentration of DNA is important for high-throughput genetic analysis and disease diagnosis. Glass-based microfilters are popular but the process requires centrifugation steps with cumbersome chemical processes. As an alternative, a concentration method using an electric field has been explored previously, but with limited efficiency. In this paper, electric field-induced concentration and capture of DNA are studied by using high-aspect-ratio microtips coated with a gold layer. The microtips are immersed longitudinally into a solution of 100???L containing ??-phage DNA. After DNA concentration using an electric field, the microtips are withdrawn from the solution. Under AC- and biased AC fields, DNA is concentrated by electrophoresis (EP), dielectrophoresis (DEP), and electroosmotic flow (EOF). To reduce capillary effects in the withdrawal process, the microtips are coated with positively charged poly-l-lysine (PLL). The pattern of captured DNA is analyzed by fluorescence microscopy. DEP attracts DNA molecules at the edges of microtips, where the highest gradient of electric field exists. EP attracts DNA onto the surface of microtips following the vectors of an electric field. EOF generates vortexes that deliver DNA onto microtips. Using this method, 85% of DNA is captured on the PLL-coated microtips after three sequential captures. The concentration mechanism can potentially facilitate rapid and simple preparation of DNA for downstream analysis.     

Fabrication of a biocompatible flexible electroosmosis micropump

In in-vivo microsystems, one of the components is a biocompatible micropump in order to produce the necessary force to deliver the fluid from the inlet to the outlet. In this contribution, a flexible micropump is fabricated which is aimed to be suitable in drug delivery applications. It provides high degree of biocompatibility, since the only employed materials are implantation grade polydimethylsiloxane elastomer and gold for the electrical interconnects. The working principle of the micropump is based on transverse DC electroosmosis which is a new variant of conventionally applied high voltage DC electroosmosis. This new technique is based on topography irregularities introduced in the channel resulting in a non-uniform charge distribution. The advantage is to drive the micropump using a relatively low DC voltage of 10 V while getting an effective flow speed of 60 μm/s. In order to characterize the flow speed, dyed 3 μm beads are dispersed in the working fluid and their speed is measured by the line scanning technique using a confocal microscope. It is also observed that the flow has a helical profile which is an attractive feature for an efficient micro-mixer in active microfluidics and μ-TAS applications.     

Millisecond-order rapid micromixing with non-equilibrium electrokinetic phenomena

We report an active micromixer utilizing vortex generation due to non-equilibrium electrokinetics near micro/nanochannel interfaces. Its design is relatively simple, consisting of a U-shaped microchannel and a set of nanochannels. We fabricated the micromixer just using a two-step reactive ion etching process. We observed strong vortex generation in fluorescent microscopy experiments. The mixing performance was evident in a combined pressure-driven and electroosmotic flows, compared with the case with a pure pressure-driven flow. We characterized the micromixer for several conditions: different applied voltages, ion concentrations, flow rates, and nanochannel widths. The experimental results show that the mixing performance is better with a higher applied voltage, a lower ion concentration, and a wider nanochannel width. We quantified the mixing characteristics in terms of mixing time. The lowest mixing time was 2 milliseconds with the voltage of 230 V and potassium chloride solutions of 0.1 mM. We expect that the micromixer is beneficial in several applications requiring rapid mixing.     

Numerical modeling of multiphase flows in microfluidics and micro process engineering: a review of methods and applications

This article presents a comprehensive review of numerical methods and models for interface resolving simulations of multiphase flows in microfluidics and micro process engineering. The focus of the paper is on continuum methods where it covers the three common approaches in the sharp interface limit, namely the volume-of-fluid method with interface reconstruction, the level set method and the front tracking method, as well as methods with finite interface thickness such as color-function based methods and the phase-field method. Variants of the mesoscopic lattice Boltzmann method for two-fluid flows are also discussed, as well as various hybrid approaches. The mathematical foundation of each method is given and its specific advantages and limitations are highlighted. For continuum methods, the coupling of the interface evolution equation with the single-field Navier–Stokes equations and related issues are discussed. Methods and models for surface tension forces, contact lines, heat and mass transfer and phase change are presented. In the second part of this article applications of the methods in microfluidics and micro process engineering are reviewed, including flow hydrodynamics (separated and segmented flow, bubble and drop formation, breakup and coalescence), heat and mass transfer (with and without chemical reactions), mixing and dispersion, Marangoni flows and surfactants, and boiling.     

2D system approach based output feedback repetitive control for uncertain discrete-time systems

This paper presents a two-dimensional (2D)-based approach to the problem of output feedback repetitive control for uncertain discrete-time systems. It is shown first how the proposed repetitive control scheme can be equivalently formulated in the form of a distinct class of 2D system. Then sufficient conditions for the existence of output feedback control law are derived in terms of linear matrix inequality (LMI), and the control law matrices are characterized by the feasible solutions to this LMI. Moreover, an optimization problem is introduced to efficiently solve the optimal output feedback control law by minimizing the upper bound of a given cost function. Compared with the commonly used 1D-based method, the proposed approach increases the degree of freedom of controller design by not only including in the scheme an output feedback gain but also a feed forward one which can be simultaneously solved using the 2D-based procedures presented in the paper. Finally, a numerical example is provided to demonstrate the effectiveness of the proposed method.     

Parameter identification and design of a robust attitude controller using <Emphasis Type="Italic">H</Emphasis><Subscript>∞</Subscript> methodology for the raptor E620 small-scale helicopter

Rigorous control synthesis for an unmanned aerial vehicle necessitates the availability of a good, reasonable model for such a vehicle. The work reported in this paper focuses on the modeling of a rotary unmanned aerial vehicle (RUAV) and the development of a robust controller that can handle parameter uncertainties and disturbances. The parameters of the plant model are obtained through the use of the prediction error method with real flight data. The response of the identified linear model shows a good match with the measured flight data. The H _∞ control scheme is applied to obtain a robustly stable controller using the identified model. The proposed controller is analyzed using frequency-domain analysis and time-domain simulations. The performance of the proposed H _∞ controller is better than that of the conventional proportional derivative controller in that the proposed controller has a shorter settling time and less overshoot. Furthermore, the degradation of the proposed controller performance is negligible and stability is maintained when the input gains to the plant are doubled, which demonstrates the good performance and robustness of the controller.     

An investigation into vehicle rollover prevention by coordinated control of active anti-roll bar and electronic stability program

This paper presents a method for designing a controller that uses an active anti-roll bar (AARB) and an electronic stability program (ESP) for rollover prevention. ESP with longitudinal speed control (LSC) can carry out active braking to reduce vehicle speed and lateral acceleration to prevent a rollover. To enhance the rollover prevention capability of the ESP, an AARB is adopted. The controller for the AARB was designed based on linear quadratic (LQ) static output feedback (SOF) control methodology, which attenuates the effect of lateral acceleration on the roll angle and roll rate by control of the suspension stroke and the tire deflection of the vehicle. Although this AARB significantly increases ride comfort and rollover prevention, it has a drawback — the vehicle loses its maneuverability. Therefore, the ESP with LSC is used to overcome this drawback. Simulations showed that the proposed method was effective in preventing a rollover.     

协调的分布式决策虚拟企业风险管理模型

虚拟企业要取得预期的利润,就必须成功规避运行中的风险.考虑虚拟企业的分布性以及联盟成员之间关系多样性的特点,根据非合作完全信息静态博弈理论,采用分布式决策的思想对虚拟企业风险进行管理.针对各盟员构成非团队关系,并且不存在机会主义行为的虚拟企业,探讨了其风险管理的特点,以及在风险管理问题上各盟员之间可能达成的协作;建立了协调的分布式虚拟企业风险管理模型,给出了模型的求解方法,同时设计了相应算法对模型进行求解,并对解的不同情况进行了分析;另外还比较了不同协调原则下联盟总利润以及各盟员利润的变化情况.仿真分析表明,所提出的模型和算法,能在虚拟企业各盟员风险管理中起到协调控制的作用,达到“双赢”的目的.     

Synthesis of hydrous SnO2 and SnO2-coated TiO2 powders by the homogeneous precipitation method and their characterization

Hydrous SnO₂ and SnO₂-coated TiO₂ powders were synthesized by the homogeneous precipitation method using urea and the products were characterized by X-ray diffraction, thermogravimetry, differential thermal analysis, scanning electron microscopy and energy- dispersive X-ray microanalysis. Electrical conductivities were measured with an impedance analyser. Hydrous SnO₂ powder prepared under conditions without SO ₄ ^2– ions was a bulky product containing 75 wt% of water. The addition of SO ₄ ^2– ions to the solution changed bulky hydrous SnO₂ to a dense product; approximately spherical particles were obtained with an average particle size of 0.14 ± 0.03 m. with 13.5 wt % of absorbed water. Antimony-doped hydrous SnO₂ prepared under conditions with SO ₄ ^2– ions consisted of approximately spherical particles with an average particle size of 0.17 ± 0.04 m with 15.0 wt % of absorbed water. Hydrous SnO₂-coated TiO₂ powders with a good dispersion state and with various Sn/Ti ratios were prepared under conditions with SO ₄ ^2– ions. All the as-prepared coated powders were white, but the products doped with Sb³⁺ ions were turned to pale blue by heat treatment at 600° C for 1 h and their electrical conductivities increased by orders of about 3.0 in comparison with those of the other two.