网络制造的安全防范措施

文章根据制造网络的特点,将制造网络划分为企业内联网、企业外联网和合作伙伴网三种层次,针对不同的制造网络采取不同的安全防范措施以保证制造网络的安全.     

Type II regulatory subunits are not required for the anchoring-dependent modulation of Ca2+ channel activity by cAMP-dependent protein kinase

Preferential phosphorylation of specific proteins by cAMP-dependent protein kinase (PKA) may be mediated in part by the anchoring of PKA to a family of A-kinase anchor proteins (AKAPs) positioned in close proximity to target proteins. This interaction is thought to depend on binding of the type II regulatory (RII) subunits to AKAPs and is essential for PKA-dependent modulation of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate receptor, the L-type Ca2+ channel, and the KCa channel. We hypothesized that the targeted disruption of the gene for the ubiquitously expressed RIIalpha subunit would reveal those tissues and signaling events that require anchored PKA. RIIalpha knockout mice appear normal and healthy. In adult skeletal muscle, RIalpha protein levels increased to partially compensate for the loss of RIIalpha. Nonetheless, a reduction in both catalytic (C) subunit protein levels and total kinase activity was observed. Surprisingly, the anchored PKA-dependent potentiation of the L-type Ca2+ channel in RIIalpha knockout skeletal muscle was unchanged compared with wild type although it was more sensitive to inhibitors of PKA-AKAP interactions. The C subunit colocalized with the L-type Ca2+ channel in transverse tubules in wild-type skeletal muscle and retained this localization in knockout muscle. The RIalpha subunit was shown to bind AKAPs, although with a 500-fold lower affinity than the RIIalpha subunit. The potentiation of the L-type Ca2+ channel in RIIalpha knockout mouse skeletal muscle suggests that, despite a lower affinity for AKAP binding, RIalpha is capable of physiologically relevant anchoring interactions.     

5%"神农丹"等3种农药防治烟草害虫的药效试验

大田对比试验表明 ,5 %“神农丹”和 15 %“铁灭克”在移栽时穴施和旺长期追施 ,对烟蚜及烟草根结线虫有很强的杀伤作用 ,无翅蚜虫口减退率可达 10 0 % ,移栽时穴施 15 %“铁灭克”1.0kg/6 6 6 .7m2 、5 %“神农丹”3.0kg/6 6 6 .7m2 对烟草根结线虫相对防效达 80 %以上 ,两种药剂防治效果差异不大     

发明问题解决理论与机械类本科生创新能力的培养

大学生创新能力的培养是高等教育阶段必须完成的重要目标。科学创新方法的选择与应用是落实这一培养目标的关键。笔者将发明问题解决理论引入到机械设计课程体系中,面向机械产品设计的全过程构建学生创新能力的培养模式。本文总结了济南大学开展发明问题解决理论教育的教学实践过程,探讨了机械类本科学生创新能力培养的基本思路和有效途径。     

一种14位、1.4 MS/s、多位量化的级联型ΣΔ调制器

在0.6 μm CMOS工艺条件下设计了一种适合DECT(Digital Enhanced Cordless Telephone)标准的1.4 MS/s Nyquist转换速率、14位分辨率模数转换器的ΣΔ调制器.该调制器采用了多位量化的级联型(2-1-14b)结构,通过Cadence SpectreS仿真验证,在采样时钟为25 MHz和过采样率为16的条件下,该调制器可以达到86.7 dB的动态范围,在3.3 V电源电压下其总功耗为76 mW.     

Distribution of protein kinase C isoforms after infection of macrophages with Leishmania major

We characterized the effects of Leishmania infection on activation-induced translocation of protein kinase C (PKC) isoforms in murine bone marrow-derived macrophages. Although PKC-dependent gene expression was attenuated by infection, the distribution and translocation of PKC isoforms were unaffected. However, subsequent dissociation from membranes was substantially delayed for some isoforms, particularly PKCbeta.     

The pulmonary uptake of intravenously administered liposomal alpha-tocopherol is augmented in acute lung injury

The present study was carried out to investigate whether the intravenous administration of liposomal alpha-tocopherol can result in a significant localization of the antioxidant in the injured lung. Male Sprague-Dawley rats were injected with paraquat dichloride (20 mg/kg, ip.) and 4, 24 or 48 h later, they were given an intravenous injection of a liposomal alpha-tocopherol preparation (20 mg alpha-tocopherol in 128 mumoles liposomal lipid/kg) labelled with [14C]dipalmitoylphosphatidylcholine (DPPC) and [3H]alpha-tocopherol. Animals were killed and their lungs removed for analysis 24 h after liposomal treatment. To demonstrate whether the extent of uptake of radioactive alpha-tocopherol liposomes was directly related to the extent of residual lung injury, additional groups of animals were also injected with higher doses (30 and 40 mg/kg body weight) of paraquat dichloride and 48 h later, were treated with liposomal alpha-tocopherol; animals were then killed 24 h after liposomal alpha-tocopherol treatment. The intraperitoneal injection of paraquat dichloride resulted in time- and dose-dependent decreases in angiotensin converting enzyme and alkaline phosphatase activities suggesting that the toxicant injures both the capillary endothelial cells and alveolar type II epithelial cells, respectively. The recovery of intravenously administered radioactive alpha-tocopherol in the lungs of saline-treated animals was found to be about 2% of the initial dose 24 h post-liposomal treatment. However, in paraquat-treated animals, there was an increased localization of the labelled alpha-tocopherol to the lung, resulting in a difference of pulmonary delivery by as much as 2-3 fold compared to that in a normal lung. The 3H/14C ratio, representing the recovery of [3H]alpha-tocopherol and [14C]liposomes, was practically constant and there was a linear relationship between the measurable lung injury index and the corresponding recovery of radiolabelled alpha-tocopherol in the lung. Our results appear to suggest that the residual pulmonary injury augments the delivery of liposomal alpha-tocopherol to the lung.     

The effect of sulfhydryl reagents on the tityustoxin-induced acetylcholine release in rat brain slices

The effect of breast feeding on serum FSH and LH concentrations was studied in 13 puerperal women. Despite the collateral increase in serum prolactin levels, nursing was not followed by significant changes in serum FSH or LH levels during a 30-min observation period. Our data indicate that nursing has no significant effect on pituitary FSH and LH release.