Intercellular junctions in FANFT-induced carcinomas of rat urinary bladder in tissue culture: in situ thin-section, freeze-fracture, and scanning electron microscopy studies.

This paper describes a set of simple methods for comparative light and electron microscopy studies on tissue cultured tumour cells derived from both noninvasive and invasive carcinogen-induced rat urinary bladder carcinomas. Cells are grown on Thermanox plastic coverslips and fixed in situ. Each plastic coverslip is then divided with scissors into four parts: the first is processed for light microscopy, the second for thin-section electron microscopy, the third for freeze-fracture electron microscopy, and the fourth for scanning electron microscopy. In some experiments, portions of the culture which have first been examined by light microscopy are subsequently prepared for electron microscopy. In this way, the culture conditions are kept constant and comparison of structural features (i.e. intercellular junctions) by several preparative techniques is possible. Noninvasive and invasive rat bladder tumour cells, characterized by numerous pleomorphic microvilli, have normal zonulae occludentes at the apices of lateral surfaces of tumour cells in all cultures. In some areas of invasive tumour cells, occludens junctions are focally attenuated, consisting of only one or two strands, and occasionally the strands are discontinuous. Gap junctions, type PF-1, as well as numerous demosomes are present in all cell lines. Thus, intercellular junctions in noninvasive and invasive rate bladder epithelial cell lines bear a striking resemblance to those previously described in the comparable solid primary tumours. These culture systems may be useful for studying factors which influence the formation of intercellular junctions during malignant transformation.     

Stereochemical Discrimination at Organic Crystal Surfaces 1: The Systems Serine/Threonine and Serine/Allothreonine

The stereoselective interactions in aqueous solution between the crystal faces of centrosymmetric (R,S) serine 1 and additives resolved and racemic threonine 2 and allothreonine 3 were studied by four independent techniques: change in crystal habit on growth, anisotropic distribution of occluded additive during the crystal growth, etch pit formation on partial dissolution and atom-atom potential energy calculations. The effects of the two diastereomers 2 and 3 are radically different due to differences in adsorption: threonine is adsorbed on the {011} crystal faces; the (R) enantiomer is adsorbed at, and eventually occluded through the (011) and (011) crystal faces replacing an (R) serine molecule. By symmetry, (S) threonine is adsorbed and occluded through the enantiotopic (011) and (011) faces replacing (S) serine. In contrast, (R) and (S) allothreonine are both adsorbed primarily at the (100) and (100) faces. The results indicate that the serine moiety of adsorbed threonine and allothreonine assume the same conformation and position as the substrate molecule. The additive is adsorbed only at specific sites such that the C_β-CH₃ groups emerge from the crystal surface. The observed effects lead in the case of threonine, but not allothreonine, to the assignment of its absolute configuration.     

Effects of curtailment on an admissions model for a graduate management program.

Developed 2 admissions models to predict future academic performance of graduate management students. The 1st model was based on 40 students who were admitted and enrolled in the program and was uncorrected for curtailment. The 2nd model was developed from the total applicant population of 222 students after curtailment correction. The corrected model demonstrated higher predictive validity than the uncorrected model for 2 future classes of students. Furthermore, different predictors entered each model, affecting the beta weights, validities of predictors, and the total amount of variance explained by the models. A factor analysis and an analysis of admissions decisions offer additional support for the curtailment-corrected model in selecting students with high academic potential. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A Simple Technique for Enhancing the Hypothesis Testing Capability of Devices Sensitive to Phase in the Presence of Narrow-Band Normal Noise

A new statistic is described for hypothesis testing on phase in the presence of narrow-band normal noise when the true phase, itself, is a nonuniformly distributed random variable. Comparisons are made with the more standard direct techniques.     

On the determination of the post-irradiation time from the glow curve of TLD-100

The ratio of peak 3 to the sum of peaks 4 + 5 in TLD-100 was measured for various pre-irradiation and post-irradiation time periods, under conditions characteristic of routine personal dosimetry. It was confirmed that the value of this ratio depends only on the elapsed time between the prior readout and the present one, independent of the moment when the irradiation took place during the total time interval (storage time). This effect indicates that fading of peak 3 seems to be due mainly to changes in the unoccupied traps, and not to decay of trapped charges, being almost independent of the presence of electrons or holes in the traps. This observation leads to the conclusion that the suggestions in the past to use the decay of peak 3 in TLD-100 for the measurement of the elapsed time between irradiation and readout may have been wrong. On the other hand, the decay of peak 2 can be used to measure the elapsed time from irradiation, since the rate of decay is different when related to pre-irradiation and post-irradiation times, indicating a much higher decay rate of the trapped charges (Randall-Wilkins decay). However, because of the fast decay rate of peak 2, its use for determination of the elapsed time since irradiation is of little practical significance.     

Genes Involved in the Endoplasmic Reticulum N-Glycosylation Pathway of the Red Microalga Porphyridium sp.: A Bioinformatic Study

N-glycosylation is one of the most important post-translational modifications that influence protein polymorphism, including protein structures and their functions. Although this important biological process has been extensively studied in mammals, only limited knowledge exists regarding glycosylation in algae. The current research is focused on the red microalga Porphyridium sp., which is a potentially valuable source for various applications, such as skin therapy, food, and pharmaceuticals. The enzymes involved in the biosynthesis and processing of N-glycans remain undefined in this species, and the mechanism(s) of their genetic regulation is completely unknown. In this study, we describe our pioneering attempt to understand the endoplasmic reticulum N-Glycosylation pathway in Porphyridium sp., using a bioinformatic approach. Homology searches, based on sequence similarities with genes encoding proteins involved in the ER N-glycosylation pathway (including their conserved parts) were conducted using the TBLASTN function on the algae DNA scaffold contigs database. This approach led to the identification of 24 encoded-genes implicated with the ER N-glycosylation pathway in Porphyridium sp. Homologs were found for almost all known N-glycosylation protein sequences in the ER pathway of Porphyridium sp.; thus, suggesting that the ER-pathway is conserved; as it is in other organisms (animals, plants, yeasts, etc.).     

Interlingua-based English–Korean Two-way Speech Translation of Doctor–Patient Dialogues with CCLINC

Development of a robust two-way real-time speech translationsystem exposes researchers and system developers to various challenges of machine translation(MT) and spoken language dialogues. The need for communicating in at least two differentlanguages poses problems not present for a monolingual spoken language dialogue system,where no MT engine is embedded within the process flow. Integration of various componentmodules for real-time operation poses challenges not present for text translation. In this paper,we present the CCLINC (Common Coalition Language System at Lincoln Laboratory) English–Koreantwo-way speech translation system prototype trained on doctor–patient dialogues,which integrates various techniques to tackle the challenges of automatic real-time speechtranslation. Key features of the system include (i) language–independent meaning representation which preserves the hierarchicalpredicate–argument structure of an input utterance, providing a powerful mechanism for discourse understanding of utterances originating from different languages,word-sense disambiguation and generation of various word orders of many languages, (ii) adoptionof the DARPA Communicator architecture, a plug-and-play distributed system architecturewhich facilitates integration of component modules and system operation in real time, and (iii)automatic acquisition of grammar rules and lexicons for easy porting of the system to differentlanguages and domains. We describe these features in detail and present experimental results.     

A Universal Method of Scientific Inquiry

A paradigm of scientific discovery is defined within a first-order logical framework. Within this paradigm, the concept of successful scientific inquiry is formalized and investigated. Among other results, it is shown that a simple method of scientific inquiry is universal in the sense that it leads to success on every problem for which success is in principle possible.     

Syndrome measurement order for the [[7,1,3]] quantum error correction code

In this work, we explore the accuracy of quantum error correction depending of the order of the implemented syndrome measurements. CSS codes require that bit-flip and phase-flip syndromes be measured separately. To comply with fault-tolerant demands and to maximize accuracy, this set of syndrome measurements should be repeated allowing for flexibility in the order of their implementation. We examine different possible orders of Shor-state and Steane-state syndrome measurements for the [[7,1,3]] quantum error correction code. We find that the best choice of syndrome order, determined by the fidelity of the state after noisy error correction, will depend on the error environment. We also compare the fidelity when syndrome measurements are done with Shor states versus Steane states and find that Steane states generally, but not always, lead to final states with higher fidelity. Together, these results allow a quantum computer programmer to choose the optimal syndrome measurement scheme based on the system’s error environment.