Fabrication of microfluidic networks with integrated electrodes

In this paper a method is presented for the fabrication of micro-channel networks in glass with integrated and insulated gate electrodes to control the zeta-potential at the insulator surface and therewith the electro-osmotic flow (EOF). The fabrication of the electrodes is a sequence of photolithography, etching and thin film deposition steps on a glass substrate, followed by chemical mechanical polishing (CMP) and subsequently direct thermal bonding to a second glass plate to form closed micro-channels. Plasma enhanced chemical vapor deposition (PECVD) SiO₂-layers as insulating material between the electrodes and micro-channels and different electrode materials are examined with respect to a high bonding temperature to obtain an optimal insulating result. A CMP process for the reduction of the SiO₂ topography and roughness is studied and optimized in order to obtain a surface that is smooth enough to be directly bondable to a second glass plate.     

A dynamic programming approach for economic optimisation of lifetime-extending maintenance,renovation, and replacement of public infrastructure assets under differential inflation

In the next decades, many public infrastructure assets will reach the end of their life that they were originally designed for. Replacement costs are high, and therefore increasing effort is put into lifetime-extending maintenance, including major overhauls and renovations. A key question is whether the investments in lifetime-extending maintenance justify the postponement of a full replacement. This question becomes more complicated when future life cycle cash flows are non-repeatable. Differential inflation and technological change, including multiple intervention strategies to maintain a desired functionality, cause such non-repeatability. In this case, classic replacement analysis techniques will not suffice in answering this question. Literature demonstrates that case-specific modelling with dynamic or linear programming techniques is required to find economic optimisation. However, such literature primarily addresses replacement interval optimisation of new investments within relative short time horizons, whereas the current research develops a nested dynamic programming (DP) approach for typical ageing infrastructure assets over long service life periods. The model can deal with multiple and various successive intervention strategies and addresses differential inflation and age-related cost increases. Finally, it is shown in an infrastructure case study that this DP approach leads to a better decision in comparison to the application of classical replacement techniques.     

Untangling decision tree and real options analyses: a public infrastructure case study dealing with political decisions,structural integrity and price uncertainty

Managerial flexibility in infrastructure investment and replacement decisions adds value. Real options analysis (ROA) captures this value under uncertain market prices. The concept of ROA is that future unfavourable payoffs can be deferred as soon as more information about market prices becomes available. The popularity of ROA is seen in a growing number of case studies on real assets. Despite its increasing popularity, ROA has not gained a foothold in public infrastructure decision making. One of the difficulties in the application of ROA is the required estimation of market variables. To avoid this, a simplified but not correct version of ROA is easily applied, referred to as a Decision Tree Approach (DTA) to ROA. Another difficulty is that infrastructure assets are subject to other types of uncertainties, defined here as asset uncertainties. This study investigates the value of managerial flexibility in a public infrastructure replacement decision. The uncertainty drivers are the strength of a bridge, political decisions regarding traffic flow and the price development of construction costs. Three valuation approaches are compared: DTA, ROA and the DT approach to ROA. Although it is complex, ROA certainly adds value in public infrastructure decision making when market price uncertainty is prevalent. However, in the absence of reasonable estimates of market variables, the DT approach to ROA is the best alternative. In the absence of market price uncertainties, ROA should be avoided DTA is to be preferred.     

Using the past to indicate the possible presence of relics in the present-day landscape: the Western Front of the Great War in Belgium

The First World War (WWI) had a notable influence on the landscape at the former Western Front in Belgium. Research on a landscape scale is necessary to understand the destructive and constructive impact of the war and its consequences for the post-war landscape in a holistic manner. This paper focuses on the trajectory and impact analysis of three study areas with contrasting landscape types and aims to indicate possible preserved military relics today. Therefore, landscape changes are studied using historical aerial photographs (WWI and WWII) and contemporary orthophotos. A military landscape characterisation is made based on land use/land cover and linear structures that were mapped throughout time. Specific landscape trajectories could be designated as areas with a possible preservation of WWI heritage, with a related impact degree. The results are useful for sustainable heritage management and for further interdisciplinary research on WWI-heritage, by providing a broad knowledge of the area.     

Native Liquid Chromatography and Mass Spectrometry to Structurally and Functionally Characterize Endo-Xylanase Proteoforms

Xylanases are of great value in various industries, including paper, food, and biorefinery. Due to their biotechnological production, these enzymes can contain a variety of post-translational modifications, which may have a profound effect on protein function. Understanding the structure–function relationship can guide the development of products with optimal performance. We have developed a workflow for the structural and functional characterization of an endo-1,4-β-xylanase (ENDO-I) produced by Aspergillus niger with and without applying thermal stress. This workflow relies on orthogonal native separation techniques to resolve proteoforms. Mass spectrometry and activity assays of separated proteoforms permitted the establishment of structure–function relationships. The separation conditions were focus on balancing efficient separation and protein functionality. We employed size exclusion chromatography (SEC) to separate ENDO-I from other co-expressed proteins. Charge variants were investigated with ion exchange chromatography (IEX) and revealed the presence of low abundant glycated variants in the temperature-stressed material. To obtain better insights into the effect on glycation on function, we enriched for these species using boronate affinity chromatography (BAC). The activity measurements showed lower activity of glycated species compared to the non-modified enzyme. Altogether, this workflow allowed in-depth structural and functional characterization of ENDO-I proteoforms.     

从青浦到嘉定 大都市无序蔓延中的先进规划

随着空前规模与速度的城市化进程,在上个十年中,上海周边的乡村地区发生了惊人的变化。在离散化的政策下,许多新城与郊区依次落成。每一个地区独特的物质条件与规章制度形成新区难以预计的多样性,同时也造成空间与功能性品质的多样化。大多数新区都依据标准步骤进行开发。一些试点新城的开发通过文本形式在西方设计师的帮助下适应中国的规范;另一些新城与街区由来自中国的新锐建筑师与规划师设计。本文主要介绍嘉定与青浦区的发展成果。     

Reinvestigation of the antioxidant properties of conjugated linoleic acid

Despite repeated suggestions that antioxidant activity of conjugated linoleic acid (CLA), a collective of conjugated dienoic isomers of linoleic acid, underlies its reported anticarcinogenic and antiatherosclerotic effects, the antioxidant properties of CLA remain ill-defined. Therefore, this study was undertaken to gain more insight into the mechanism of potential CLA antioxidant activity. It was tested whether CLA could protect membranes composed of 1-palmitoyl-2-linoleoyl phosphatidylcholine (PLPC) from oxidative modification under conditions of metal ion-dependent or-independent oxidative stress. Progress of oxidation was determined by direct spectrophotometric measurement of conjugated diene formation and by gas chromatographic/mass spectrometric analysis of fatty acids. The oxidative susceptibility of CLA was higher than that of linoleic acid, and comparable to arachidonic acid. When oxidation of PLPC (1.0 mM) was initiated using the lipid-soluble 2,2′-azobis(2,4-dimethylvaleronitrile) or the water-soluble 2,2′-azobis(2-amidinopropane) hydrochloride, the radical scavengers vitamin E and butylated hydroxytoluene (BHT) at 0.75 μM efficiently inhibited PLPC oxidation, as evident from a clear lagphase. In contrast, 0.75 μM CLA did not have any significant effect on PLPC oxidation. Inhibition of PLPC oxidation by higher concentrations of CLA appeared due to competition, not to an antioxidant effect. When oxidation of PLPC was initiated by hydrogen peroxide/Fe²⁺ (500 μM/0.05–20 μM), both vitamin E (1 μM) and ethylene glycol-bis(aminoethyl ether) tetraacetic acid (50 μM) efficiently inhibited PLPC oxidation. However, CLA (1–50 μM) did not show a clear protective effect under any of the conditions tested. We conclude that CLA, under these test conditions, does not act as an efficient radical scavenger in any way comparable to vitamin E or BHT. CLA also does not appear to be converted into a metal chelator under metal-ion dependent oxidative stress, as had previously been suggested. On the basis of our observations, a role for CLA as an antioxidant does not seem plausible.     

Microstructural analysis of MTR fuel plates damaged by a coolant flow blockage

In 1975, as a result of a blockage of the coolant inlet flow, two plates of a fuel element of the BR2 reactor of the Belgian Nuclear Research Centre (SCK•CEN) were partially melted. The fuel element consisted of Al-clad plates with 90% ²³⁵U enriched UAl_x fuel dispersed in an Al matrix. The element had accumulated a burn up of 21% ²³⁵U before it was removed from the reactor. Recently, the damaged fuel plates were sent to the hot laboratory for detailed PIE.Microstructural changes and associated temperature markers were used to identify several stages in the progression to fuel melting. It was found that the temperature in the center of the fuel plate had increased above 900-950 °C before the reactor was scrammed. In view of the limited availability of such datasets, the results of this microstructural analysis provide valuable input in the analysis of accident scenarios for research reactors.