Delaminations at the free edge of a composite laminate

The concept of a fracture process zone where damage takes place is used to analyse the delaminations at the free edges of angle ply laminates under uniaxial tension. The use of a fracture process zone removes the singularity in the interlaminar stresses and enables the initiation and growth of delaminations to be modelled for a perfect laminate without any assumed prior defects. Two different models for the stress displacement relationship in the fracture process zone are examined: a constant stress up to critical displacement and a linear relationship. Finite element analysis shows that there is little difference in the predictions obtained from the two models. An approximate analysis is presented for the constant stress stress-displacement model which is shown to agree with a finite element solution and experimental data. Hence it is argued that the approximate method using a constant stress model for the fracture process zone is sufficient for accurate prediction of delaminations.     

Evaluating a graphical notation for modelling software development methodologies

This work aims at evaluating a graphical notation for modelling software (and other kinds of) development methodologies, thus demonstrating how useful the graphical aspects can be for sharing knowledge between the people responsible for documenting information and those responsible for understanding and putting it into practice. We acknowledge the importance of having a common set of symbols that can be used to create, use and disseminate information for a larger audience than is possible today with a variety of alternatives and lack of a common ground. Using a cognitive dimensions framework, we make a standard evaluation of the elements and diagrams of the notation proposed to support the ISO/IEC 24744 methodology metamodel standard, considering the trade-offs between different dimensions. We suggest improvements to this existing notation based on this analysis, in the context of improving communication between creators and users of methodologies.     

Performance Evaluation of Range Queries in Key Value Stores

Recently there has been a considerable increase in the number of different Key-Value stores, for supporting data storage and applications on the cloud environment. While all these solutions try to offer highly available and scalable services on the cloud, they are significantly different with each other in terms of the architecture and types of the applications, they try to support. Considering three widely-used such systems: Cassandra, HBase and Voldemort; in this paper we compare them in terms of their support for different types of query workloads. We are mainly focused on the range queries. Unlike HBase and Cassandra that have built-in support for range queries, Voldemort does not support this type of queries via its available API. For this matter, practical techniques are presented on top of Voldemort to support range queries. Our performance evaluation is based on mixed query workloads, in the sense that they contain a combination of short and long range queries, beside other types of typical queries on key-value stores such as lookup and update. We show that there are trade-offs in the performance of the selected system and scheme, and the types of the query workloads that can be processed efficiently.     

Two impossibility theorems on behaviour specification of abstract data types

Two kinds of finite specification of the behaviour of a counter data type are proved impossible.We consider the class of data types (many-sorted algebras) behaving like an encapsulated counter that can be observed only by a test for zero. It is shown that no nonempty subclass of this class can be finitely specified in observational first-order logic, which is a variant of first-order logic in which equality may not be used on encapsulated types. Secondly, it is shown that the class cannot be described exactly by a finite specification in first-order logic.An extended abstract of a part of this paper appeared as: Schoett, O.: An observational subset of first-order logic cannot speacify the behaviour of a counter, in: Choffrut, C., Jantzen, M. (eds) STACS 91. 8th Annual Symposium on Theoretical Aspects of Computer Science (Lect. Notes comput. Sci., vol. 480, pp. 499–510) Berlin Heidelberg New York: Springer 1991     

The dielectric response of doped semiconducting glasses

Measurements are reported of the dielectric properties of two semiconducting glasses of composition SiO₂ · Na₂O with admixtures of Sb_{2O 5} and SnO₂, covering a wide range of temperatures and a range of six decades of frequency 10^–2 to 10⁴ Hz. Both glasses show a gradual evolution of growing low frequency dispersion (LFD) with increasing temperature, with both the real and imaginary components of the complex susceptibility following a power law of the type ^n–1, with n 1 at the lowest frequencies. There are also clear indications of reversible phase transitions taking place which manifest themselves by changes in the spectral shape of the dielectric response.     

Polycyclic Aromatic Hydrocarbons Activate the Aryl Hydrocarbon Receptor and the Constitutive Androstane Receptor to Regulate Xenobiotic Metabolism in Human Liver Cells

Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants produced by incomplete combustion of organic matter. They induce their own metabolism by upregulating xenobiotic-metabolizing enzymes such as cytochrome P450 monooxygenase 1A1 (CYP1A1) by activating the aryl hydrocarbon receptor (AHR). However, previous studies showed that individual PAHs may also interact with the constitutive androstane receptor (CAR). Here, we studied ten PAHs, different in carcinogenicity classification, for their potential to activate AHR- and CAR-dependent luciferase reporter genes in human liver cells. The majority of investigated PAHs activated AHR, while non-carcinogenic PAHs tended to activate CAR. We further characterized gene expression, protein abundancies and activities of the AHR targets CYP1A1 and 1A2, and the CAR target CYP2B6 in human HepaRG hepatoma cells. Enzyme induction patterns strongly resembled the profiles obtained at the receptor level, with AHR-activating PAHs inducing CYP1A1/1A2 and CAR-activating PAHs inducing CYP2B6. In summary, this study provides evidence that beside well-known activation of AHR, some PAHs also activate CAR, followed by subsequent expression of respective target genes. Furthermore, we found that an increased PAH ring number is associated with AHR activation as well as the induction of DNA double-strand breaks, whereas smaller PAHs activated CAR but showed no DNA-damaging potential.     

Characterization and Reconstitution of Human Lipoyl Synthase (LIAS) Supports ISCA2 and ISCU as Primary Cluster Donors and an Ordered Mechanism of Cluster Assembly

Lipoyl synthase (LIAS) is an iron–sulfur cluster protein and a member of the radical S-adenosylmethionine (SAM) superfamily that catalyzes the final step of lipoic acid biosynthesis. The enzyme contains two [4Fe–4S] centers (reducing and auxiliary clusters) that promote radical formation and sulfur transfer, respectively. Most information concerning LIAS and its mechanism has been determined from prokaryotic enzymes. Herein, we detail the expression, isolation, and characterization of human LIAS, its reactivity, and evaluation of natural iron–sulfur (Fe–S) cluster reconstitution mechanisms. Cluster donation by a number of possible cluster donor proteins and heterodimeric complexes has been evaluated. [2Fe–2S]-cluster-bound forms of human ISCU and ISCA2 were found capable of reconstituting human LIAS, such that complete product turnover was enabled for LIAS, as monitored via a liquid chromatography–mass spectrometry (LC–MS) assay. Electron paramagnetic resonance (EPR) studies of native LIAS and substituted derivatives that lacked the ability to bind one or the other of LIAS’s two [4Fe–4S] clusters revealed a likely order of cluster addition, with the auxiliary cluster preceding the reducing [4Fe–4S] center. These results detail the trafficking of Fe–S clusters in human cells and highlight differences with respect to bacterial LIAS analogs. Likely in vivo Fe–S cluster donors to LIAS are identified, with possible connections to human disease states, and a mechanistic ordering of [4Fe–4S] cluster reconstitution is evident.     

X-ray Fluorescence Uptake Measurement of Functionalized Gold Nanoparticles in Tumor Cell Microsamples

Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.