The band areas of proteins determined by fluorescent scanning in the commercial automated gel electrophoresis apparatus

An automated gel electrophoresis apparatus, recently available commercially, allows one to follow the band during electrophoresis in real time, and lends itself therefore to an evaluation of bandwidth as a function of migration time (the dispersion coefficient), resolution and band shape. These determinations assume the constancy of band area with migration time and at various gel concentrations. The purpose of the present study was to verify these assumptions. Representative proteins and sodium dodecyl sulfate (SDS)-proteins, either natively fluorescent or fluorescein carboxylate labeled, were found to exhibit band areas which approach constancy as a function of migration time in both agarose and polyacrylamide gel electrophoresis, provided that (i) the protein concentration under the band was low enough to obviate self-quenching of fluorescence; (ii) the separation of the protein of interest from contaminants had progressed sufficiently during the time at which band areas were measured; (iii) the baseline under the peak was sufficiently well defined. However, band areas decrease with increasing gel concentration. Protein peaks exhibited leading and trailing tails. The ratio of the combined tail area to total area appeared to be near-constant at varying migration times. However, that ratio increases with increasing gel concentration. The tail area does not appear to be an artifact of fluorometric detection since it is reproduced upon fluorimetric analysis of the protein eluted from gel slices after electrophoresis. However, it may be due to photochemical destruction under the conditions of repetitive fluorometric peak detection.     

Early patterning of prelimbic cortical axons to the striatal patch compartment in the neonatal mouse

The striatum receives excitatory input from virtually the entire cerebral cortex. In the adult, this input is segregated into two functionally distinct compartments of the striatum, the patch (striosome) and matrix regions. This study determined whether the patterning of corticostriatal afferents from the prelimbic cortex to the striatal patch compartment develops during the early period of collateral formation or instead at the time of peak synaptogenesis. Initial formation of corticostriatal axon collaterals was observed by embryonic day (E) 19. Quantification of corticostriatal collaterals revealed a significant increase in the number and complexity of collateral branches at postnatal day 6 as compared to E19. Concomitant with the increase in collateral branching, a heterogeneous pattern of collateralization consisting of parallel rows of corticostriatal collaterals was observed in the medial striatum. In addition to the rows, clusters of corticostriatal axons occurred more laterally. These clusters colocalized with patches of dense tyrosine hydroxylase-positive fibers, a marker for the striatal patch compartment in the neonatal mouse. Together, these data indicate that corticostriatal patterning occurs during the period of early axon collateralization resulting in a segregation of corticostriatal axon collaterals from the prelimbic cortex to the striatal patch compartment.     

大庆外围盆地侏罗纪-早白垩世早期古气候研究

为扩大勘探领域，运用古生物资料对大庆外围盆地的古气候进行了研究，外围盆地株罗纪－早白垩世早期发育的地层有早侏罗世海拉尔盆地的东宫组，中侏罗世三江盆地的绥滨断陷的绥滨组，晚侏罗世三江盆地的东荣组，虎林盆地的裴德组，七虎林组，早白垩世早期鸡西盆地，三江盆地的滴道组，城子河组、穆棱组，虎林盆地的下云山组，上云山组，在这些组中除晚株罗世东荣组，裴德组，七虎林组外，其它各组都产有比较丰富的孢粉化石，未发现孢粉化石的层位，依据植物化石，得出早株罗世东营组为潮湿的暖温带气候，中侏罗世绥滨组为潮湿的北亚热带气候，晚侏罗世为潮湿的暖温带气候，早白垩世早期滴道组，下云山组为潮湿的北亚热带气候，城子河组、上云山组为潮湿的中亚热带气候，穆棱组为潮湿的南亚热带气候。     

减粘裂化分馏塔顶系统的腐蚀及防护

辽河石化分公司减粘裂化装置改炼辽河超稠油的减压渣油后 ,分馏塔顶系统由HCl -H2 S -HCN -有机酸 -H2 O引起的电化学腐蚀严重 ,其中油水分离器局部腐蚀速率达 3.1mm/a ,12AlMoV管束的空冷器运行不足两个月即发生泄漏。塔顶系统采用BZH - 1中和缓蚀剂、无机氨BZH - 913油溶性缓蚀剂综合注剂方案 ,使腐蚀得到控制     

Trypsin Inhibitor Activity In Vitro Digestibility and Sensory Quality of Meat-Like Yuba Products as Affected by Processing

ABSTRACT: :
Soybeans ( Glycine max ) were soaked and ground to obtain soymilk. The soymilk was cooked in an open tank and held at 85 to 90 deg;C. Yuba films were picked up in 20 min intervals and dried for 20 min. Yuba films were soaked in chicken-flavor solutions (25% and 35%), and baking soda (BS) solutions (0%, 1%, 2%, and 3% BS), and cooked at 100 °C for 30 min, 60 min, and 90 min. TIA decreased (p < 0.05) with the increase of heating time and BS concentration. In vitro protein digestibility (IVPD) decreased with heating time and BS concentration (p < 0.05). Sensory characteristics were affected by flavor concentration. By using 0% BS, 25% of the chicken flavor concentration, and a short heating time method, meat-like products with low TIA, high IVPD, and good sensory characteristics were obtained.     

并行哈夫曼编码器的硬件设计与实现

文章设计了一种并行编码的哈夫曼硬件编码器，它采用了流水线和并行编码方法，使得在一个时钟周期内可以编码一个字节的数据，在编码时显著降低了工作频率。文章给出了关键部分的实现方案并分析了实验结果。     

CAPP中参数化规格表的建立及可视化填写的实现

计算机辅助工艺设计（CAPP）软件能否高效地发挥其功用，其基础数据库建立的好坏起着极其重要的作用。在对众多商品化CAPP软件进行详细调研的基础上，开发出了能由用户自行建立参数化规格表并实现可视化填写的CAPP系统，并在国家863/CF-CIMS应用示范工程中得到应用。     

机载相控阵天线辐射方向图的分析

采用一致性几何绕射理论分析了某型预警机机体对其上安装的大型相控阵天线辐射方向图的影响，计算了天线经机体散射后的场，优化了大型相控阵天线安装的最佳位置，为天线装机后辐射性能预测与布局优化提供了理论依据。     

A critical heat flux model based on mass, energy, and momentum balance for upflow boiling at low qualities

A mechanistic modeling of critical heat flux (CHF) in upflow boiling at low qualities is performed. The developed model is based on a physical criterion of CHF occurrence and a mechanism limiting the thermal transport between a stagnant bubbly layer and bulk stream. The mechanism can be mathematically formulated by coupling the equation of limiting mixing mass flux, which is derived from momentum balance equations in two regions, with local mass and energy balance equations on the bubbly layer. The resulting form of the model is represented by a general and straightforward CHF formula involving two empirical constants related to the void fraction and the thickness of the bubbly layer. The predictions agree well with the extensive CHF data of water in uniformly heated tubes.     

8Li次级束的产生

在北京串列加速器次级束流线上通过2 H(7Li,8Li) 1H逆运动学反应产生了用于核天体物理研究的8Li次级束。准直后的次级束纯度达到 80 %以上 ,对于 44MeV的7Li强度约为 3 0s- 1·pnA- 1,可以用来进行逆几何转移反应的实验测量。