A PET study of Turner's syndrome: effects of sex steroids and the X chromosome on brain

Women with Turner's syndrome (TS) allow us to study the neurobiological associates of cognitive and behavioral abnormalities because they lack one/part of one X chromosome, and endogenous estrogen. We studied 13 healthy controls (mean age +/- SD, 28 +/- 6 years) and 16 TS subjects (mean age +/- SD, 26 +/- 6 years). We measured cognitive abilities using neuropsychological tests, and cerebral metabolic rates for glucose with positron emission tomography. Compared to controls, TS subjects had significant absolute hypermetabolism in most brain areas; however, normalized metabolism was significantly lower in TS subjects than controls in the insula and association neocortices bilaterally, and there were significant differences in functional metabolic associations of brain region pairs originating in occipital cortex bilaterally, and within the right hemisphere. There were significant correlations between right-left cognitive and metabolic asymmetries in the TS group. Also, within TS a preliminary analysis demonstrated "X chromosome dosage" effects in language ability and left temporal metabolism, asymmetry of right-left test scores, and parietal metabolism. We hypothesize that within TS: i) generalized brain hypermetabolism reflects global abnormalities in neuron packing; ii) neuronal abnormalities occur in association neocortex that differ in nature or extent from whole brain and are associated with significant differences in normalized metabolism; iii) cognitive deficits are related to brain metabolic abnormalities; and iv) social-behavioral problems may be related to abnormalities of brain metabolism. Moreover, in human brain the X chromosome involved in development of the association neocortices.     

Going with the Flow: Tunable Flow‐Induced Polymer Mechanochemistry

Mechanical forces can drive chemical transformations in polymers, directing reactions along otherwise inaccessible pathways, providing exciting possibilities for developing smart, responsive materials. The state‐of‐the‐art test for solution‐based polymer mechanochemistry development is ultrasonication. However, this does not accurately model the forces that will be applied during device fabrication using processes such as 3D printing or spray coating. Here, a step is taken toward predictably translating mechanochemistry from molecular design to manufacturing by demonstrating a highly controlled nozzle flow setup in which the shear forces being delivered are precisely tuned. The results show that solvent viscosity, fluid strain rate, and the nature of the breaking bond can be individually studied. Importantly, it is shown that the influence of each is different to that suggested by ultrasonication (altered quantity of chain breakage and critical polymer chain length). Significant development is presented in the understanding of polymer bond breakage during manufacturing flows to help guide design of active components that trigger on demand. Using an anthracene‐based mechanophore, the triggering of a fluorescence turn‐on is demonstrated through careful selection of the flow parameters. This work opens the avenue for programmed chemical transformations during inline manufacturing processes leading to tunable, heterogeneous final products from a single source material.     

High-performance microwave circuits on LaAlO3

Microwave properties of high-temperature films deposited on LaAlO₃ substrate are presented. The films are grownin situ using a high-pressure single-source sputtering technique. Microwave resonators and filters are fabricated and tested. The maximum measuredQ values are 1400; the surface resistances were less than 360 between 4.2 and 50 K. The filter performance was 18 dB better than a similar filter fabricated in Ag.     

A Siphonotid Millipede (Rhinotus) as the Source of Spiropyrrolizidine Oximes of Dendrobatid Frogs

Poison frogs of the neotropical family Dendrobatidae contain a wide variety of lipophilic alkaloids, which are accumulated from alkaloid-containing arthropods. A small millipede, Rhinotus purpureus (Siphonotidae), occurs microsympatrically with the dendrobatid frog Dendrobates pumilio on Isla Bastimentos, Bocas del Toro Province, Panamá. Methanol extracts of this millipede contain the spiropyrrolizidine O-methyloxime 236, an alkaloid previously known only from skin extracts of poison frogs, including populations of D. pumilio. Thus, R. purpureus represents a likely dietary source of such alkaloids in dendrobatid frogs.     

Alkaline hydrolysis of homopolymers and copolymers of 2-hydroxypropyl methacrylate

Homopolymers of 2-hydroxypropyl methacrylate (HPMA) and copolymers with acrylic acid (AA) were prepared in 1,4-dioxane. The reactivity ratios were determined to be r_AA = 0.27 ± 0.04 and r_HPMA = 2.2 ± 0.2. The alkaline hydrolysis by sodium hydroxide of the HPMA monomer and polymers showed that while the HPMA monomer hydrolyzed readily as expected for a low-molecular-weight carboxylic ester the HPMA homopolymer and water-soluble sodium acrylate (NaA) copolymers were extremely resistant to alkaline hydrolysis. The saponification reaction followed a second-order rate equation, being first order with respect to both HPMA and hydroxide ion concentration. The Arrhenius parameters, activation energy E and frequency factor A, for the alkaline hydrolysis of HPMA monomer in water were found to be E = 10.3 Kcal/mol and A = 1.5 × 10⁸ L/mol min, and those for the NaA–HPMA copolymers in water were found to be E = 24 kcal/mol and A = 4 × 10¹² L/mol min. The NaA–HPMA copolymers had a limiting extent of hydrolysis, ranging from 9–90% ester conversion. A sharp rate decrease at low conversion was noted during the HPMA homopolymer hydrolysis in 58/42 dimethyl sulfoxide/water, allowing the calculation of two distinct reaction rates. © 1992 John Wiley & Sons, Inc.     

Bit‐depth extension: Overcoming LCD‐driver limitations by using models of the equivalent input noise of the visual system

Abstract— Continuous tone, or “contone,” imagery usually has 24 bits/pixel as a minimum, with 8 bits each for the three primaries in typical displays. However, lower‐cost displays constrain this number because of various system limitations. Conversely, higher‐quality displays seek to achieve 9–10 bits/pixel/color, though there may be system bottlenecks limited to 8. The two main artifacts from reduced bit‐depth are contouring and loss of amplitude detail; these can be prevented by dithering the image prior to these bit‐depth losses. Our technique builds on Roberts's noise‐modulation idea and the subsequently influenced work in halftoning for hardcopy and dithering for displays. However, most halftoning/dithering work was primarily directed to displays at the lower end of bits/pixel (e.g., 1 bit as in halftoning) and higher ppi. We approach the problem from the higher end of bits/pixel/color, for example, 6–8, and lower spatial resolution (<100 ppi), which changes the game substantially from halftoning experience. Instead of spatial dither, it is better to use an amplitude dither. In addition, dynamic displays allow for the use of a temporal dithering component. This paper will report on techniques and observations made in achieving contone quality on ～100‐or‐less‐ppi LCDs starting from 4‐ to 8‐bit driver limits, and resulting with no visible dither patterns, noise, contours, or loss of amplitude detail at viewing distances as close as the near focus limit (～120 mm).     

Analysis and design of a practical discontinuous-conduction-modeBIFRED converter

Impending international standards on harmonic current levels drawn by single-phase mains-operated equipment have created a need for low-cost off-line power-factor-corrected switched-mode power supply topologies in the power range up to a few hundred watts. The boost integrated/flyback rectifier/energy storage/DC-DC converter (BIFRED) is one such topology which shows promise in this regard. In particular, the discontinuous-conduction-mode (DCM) BIFRED avoids the light-load high-voltage stress problem associated with the continuous-conduction-mode design, while still achieving the combined advantages of a low-cost single-stage topology with high displacement factor and low total harmonic distortion. In this paper, a practical DCM BIFRED converter with integrated low-loss snubber is investigated from both power and small-signal control perspectives. Design equations are given to ensure DCM operation under closed-loop output voltage control, in which switch duty cycle is varying. Experimental results on a prototype converter are also presented     

Evaluating inheritance depth on the maintainability of object-oriented software

This empirical research was undertaken as part of a multi-method programme of research to investigate unsupported claims made of object-oriented technology. A series of subject-based laboratory experiments, including an internal replication, tested the effect of inheritance depth on the maintainability of object-oriented software. Subjects were timed performing identical maintenance tasks on object-oriented software with a hierarchy of three levels of inheritance depth and equivalent object-based software with no inheritance. This was then replicated with more experienced subjects. In a second experiment of similar design, subjects were timed performing identical maintenance tasks on object-oriented software with a hierarchy of five levels of inheritance depth and the equivalent object-based software.The collected data showed that subjects maintaining object-oriented software with three levels of inheritance depth performed the maintenance tasks significantly quicker than those maintaining equivalent object-based software with no inheritance. In contrast, subjects maintaining the object-oriented software with five levels of inheritance depth took longer, on average, than the subjects maintaining the equivalent object-based software (although statistical significance was not obtained). Subjects' source code solutions and debriefing questionnaires provided some evidence suggesting subjects began to experience difficulties with the deeper inheritance hierarchy.It is not at all obvious that object-oriented software is going to be more maintainable in the long run. These findings are sufficiently important that attempts to verify the results should be made by independent researchers.     

Genetic analysis of ozone-induced acute lung injury in sensitive and resistant strains of mice

Sprague-Dawley rats sedated with intraperitoneal injection of diazepam (7.5 mg/kg) were placed in a plethysmograph to measure the changes in spontaneous respiration. Inhalation of methacholine (MCh) or acetylcholine (ACh) aerosol did not alter the volume of breathing, but increased respiratory frequency (RF) to the same extent in a concentration-dependent manner. On the other hand, the tachypnea effect of MCh lasted 11 min, and that of ACh only 3 min. Urethane anesthesia inhibited spontaneous respiration and the response to MCh. Atropine, salbutamol and aminophylline inhibited MCh-induced tachypnea. In sensitized rats, the response to MCh was potentiated 6 h after inhalation of ovalbumin aerosol. The results indicate that sedation with diazepam and inhalation of MCh aerosol used in this report are suitable for measuring airway responsiveness in terms of degree of increase of respiratory frequency.     

Process Modelling and Empirical Studies of Software Evolution (PMESSE'97) Workshop Report

Much progress is being made in both the areas of process modelling and software metrics. However, neither of these concepts is complete without the other: processes cannot be improved if no assessment of quality is available, and metrics are useless if they cannot be applied in order to assess the evolution of systems. The PMESSE (Process Modelling and Empirical Studies of Software Evolution) Workshop, held in Boston MA, on May 18, 1997, brought together researchers and practitioners from both of these fields, and stimulated some very lively debate on these issues. This collection of reports reflects the work done by the Workshops five Working Groups.Dialogue between the software metrics and process modelling communities is essential. This workshop succeeded in bringing together researchers with a wide range of research interests, and the resulting discussions were very animated. Perhaps the most contentious point of discussion lay in the 'level of granularity' question; some suggested that only high-level measurements were sensible, whereas others preferred to 'divide and conquer' the system, providing detailed process measurement schemas. No doubt this debate will continue.